PMID- 32755808
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 166
DP  - 2020 Oct 15
TI  - Highly sensitive optoelectrical biosensor for multiplex allergy diagnosis.
PG  - 112438
LID - S0956-5663(20)30432-2 [pii]
LID - 10.1016/j.bios.2020.112438 [doi]
AB  - Compact multiplexed biosensors systems hold great potential for diagnosis of 
      diseases where the detection of multiple biomarkers is required. Hypersensitivity 
      Immunoglobulin E mediated syndromes are primary immunodeficiency disorders 
      associated with sensitization to allergens. Assessing immunoglobulin E (IgE) 
      sensitization to allergens is an important strategy for allergy diagnosis. Here, 
      we report for the first time a reliable, flexible and cost-effective 
      optoelectrical biosensor system for the simultaneous determination of total and 
      allergen-specific IgE and IgG, antibodies using an immunogold-silver signal 
      amplification method. The biosensor was constructed on a regular digital 
      versatile disc (DVD) to immobilize a panel of 12 allergen extracts or pure 
      proteins in microarray format, as a proof of concept. The multiplexed biosensor 
      showed a limit of detection of 0.26 IU/mL (624 pg/mL) and 14 ng/mL for IgE and 
      IgG antibodies, respectively. The system was successfully applied in a cohort of 
      127 human serum samples, showing good sensitivity (97.6%) as well as specificity 
      (85.7%), and an excellent area under the curve (AUC) value was found at 0.977 
      (confidence interval, CI 0.957 to 0.990) as compared and validated with a 
      reference clinical immunofluorescence assay, confirming an excellent correlation 
      between both techniques. The multiplex biosensor system with on-demand panel 
      composition can be used fully autonomously in clinical or mobile laboratory 
      settings without the need for any additional medical equipment, with which could 
      make it suitable for massive allergy screening campaigns to better define 
      sensitization profiles.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Mas, Salvador
AU  - Mas S
AD  - Instituto Interuniversitario de Investigacion de Reconocimiento Molecular y 
      Desarrollo Tecnologico (IDM), Universitat Politecnica de Valencia-Universitat de 
      Valencia, Spain.
FAU - Badran, Ahmed A
AU  - Badran AA
AD  - Instituto Interuniversitario de Investigacion de Reconocimiento Molecular y 
      Desarrollo Tecnologico (IDM), Universitat Politecnica de Valencia-Universitat de 
      Valencia, Spain.
FAU - Juarez, Maria-Jose
AU  - Juarez MJ
AD  - Instituto Interuniversitario de Investigacion de Reconocimiento Molecular y 
      Desarrollo Tecnologico (IDM), Universitat Politecnica de Valencia-Universitat de 
      Valencia, Spain.
FAU - Fernandez de Rojas, Dolores Hernandez
AU  - Fernandez de Rojas DH
AD  - Hospital Universitari i Politenic La Fe, Servicio de Alergia, Avinguda de 
      Fernando Abril Martorell, 106, 46026, Valencia, Spain.
FAU - Morais, Sergi
AU  - Morais S
AD  - Instituto Interuniversitario de Investigacion de Reconocimiento Molecular y 
      Desarrollo Tecnologico (IDM), Universitat Politecnica de Valencia-Universitat de 
      Valencia, Spain; Departamento de Quimica, Universitat Politecnica de Valencia, 
      Camino de Vera s/n, 46022, Valencia, Spain; Unidad Mixta UPV-La Fe, Nanomedicine 
      and Sensors, IIS La Fe, Valencia, Spain. Electronic address: smorais@qim.upv.es.
FAU - Maquieira, Angel
AU  - Maquieira A
AD  - Instituto Interuniversitario de Investigacion de Reconocimiento Molecular y 
      Desarrollo Tecnologico (IDM), Universitat Politecnica de Valencia-Universitat de 
      Valencia, Spain; Departamento de Quimica, Universitat Politecnica de Valencia, 
      Camino de Vera s/n, 46022, Valencia, Spain; Unidad Mixta UPV-La Fe, Nanomedicine 
      and Sensors, IIS La Fe, Valencia, Spain.
LA  - eng
PT  - Journal Article
DEP - 20200715
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Allergens)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - *Biosensing Techniques
MH  - Humans
MH  - *Hypersensitivity/diagnosis
MH  - Immunoglobulin E
MH  - Immunoglobulin G
OTO - NOTNLM
OT  - Allergens
OT  - Component-based diagnostics
OT  - IgE
OT  - IgG
OT  - In vitro biosensing
OT  - Multiplex
EDAT- 2020/08/07 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/08/07 06:00
PHST- 2020/05/14 00:00 [received]
PHST- 2020/07/03 00:00 [revised]
PHST- 2020/07/07 00:00 [accepted]
PHST- 2020/08/07 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/08/07 06:00 [entrez]
AID - S0956-5663(20)30432-2 [pii]
AID - 10.1016/j.bios.2020.112438 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2020 Oct 15;166:112438. doi: 10.1016/j.bios.2020.112438. 
      Epub 2020 Jul 15.