PMID- 3275593
OWN - NLM
STAT- MEDLINE
DCOM- 19880223
LR  - 20071114
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 29
IP  - 1
DP  - 1988 Jan
TI  - Cell culture of the human lamina cribrosa.
PG  - 78-89
AB  - The extracellular matrix of the lamina cribrosa may be important in the changes 
      in the optic nerve head associated with glaucoma. To investigate the cell biology 
      of this tissue, human lamina cribrosa was explanted in tissue culture and two 
      cell types grown from this tissue were characterized. The most common cell type 
      obtained was a large, flat, polygonal cell which was negative for glial fibrillar 
      acidic protein (GFAP) and could be serially subcultured. This cell type 
      synthesized collagens type III and type IV, fibronectin and elastin. Much less 
      commonly grown was a cell type with conspicuous long processes and which was 
      positive for GFAP. This presumed astrocyte synthesized collagen type IV and 
      fibronectin. Fibroblastic cells were not obtained from this tissue but were 
      easily grown from sclera. The cells that we have cultured from the human lamina 
      cribrosa may produce the extracellular matrix present in the cribriform plates of 
      this tissue and be important in the glaucomatous process.
FAU - Hernandez, M R
AU  - Hernandez MR
AD  - Ophthalmic Pharmacology Unit, Eye Research Institute, Boston, MA 02114.
FAU - Igoe, F
AU  - Igoe F
FAU - Neufeld, A H
AU  - Neufeld AH
LA  - eng
GR  - EY-06416/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Fibronectins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 9007-58-3 (Elastin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cells/classification
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - Elastin/metabolism
MH  - Fibronectins/metabolism
MH  - Fluorescent Antibody Technique
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Sclera/*cytology/metabolism/ultrastructure
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1988 Jan;29(1):78-89.