PMID- 32757397
OWN - NLM
STAT- MEDLINE
DCOM- 20210813
LR  - 20210813
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 53
IP  - 12
DP  - 2021 Jun
TI  - Poly I:C-induced maternal immune challenge reduces perineuronal net area and 
      raises spontaneous network activity of hippocampal neurons in vitro.
PG  - 3920-3941
LID - 10.1111/ejn.14934 [doi]
AB  - Activation of the maternal immune system (MIA) during gestation is linked to 
      neuropsychiatric diseases like schizophrenia. While many studies address 
      behavioural aspects, less is known about underlying cellular mechanisms. In the 
      following study, BALB/c mice received intraperitoneal injections of 
      polyinosinic-polycytidylic acid (Poly I:C) (20 microg/ml) or saline (0.9%) at 
      gestation day (GD) 9.5 before hippocampal neurons were isolated and cultured from 
      embryonic mice for further analysis. Interestingly, strongest effects were 
      observed when the perineuronal net (PNN) wearing subpopulation of neurons was 
      analysed. Here, a significant reduction of aggrecan staining intensity, area and 
      soma size could be detected. Alterations of PNNs are often linked to 
      neuropsychiatric diseases, changes in synaptic plasticity and in 
      electrophysiology. Utilizing multielectrode array analysis (MEA), we observed a 
      remarkable increase of the spontaneous network activity in neuronal networks 
      after 21 days in vitro (DIV) when mother mice suffered a prenatal immune 
      challenge. As PNNs are associated with GABAergic interneurons, our data indicate 
      that this neuronal subtype might be stronger affected by a prenatal MIA. 
      Degradation or damage of this subtype might cause the hyperexcitability observed 
      in the whole network. In addition, embryonic neurons of the Poly I:C condition 
      developed significantly shorter axons after five days in culture, while dendritic 
      parameters and apoptosis rate remained unchanged. Structural analysis of synapse 
      numbers revealed an increase of postsynaptic density 95 (PSD-95) puncta after 14 
      DIV and an increase of presynaptic vesicular glutamate transporter (vGlut) puncta 
      after 21 DIV, while inhibitory synaptic proteins were not altered.
CI  - (c) 2020 The Authors. European Journal of Neuroscience published by Federation of 
      European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Wegrzyn, David
AU  - Wegrzyn D
AD  - Department of Cell Morphology and Molecular Neurobiology, Ruhr-University Bochum, 
      Bochum, Germany.
FAU - Manitz, Marie-Pierre
AU  - Manitz MP
AD  - Division of Experimental and Molecular Psychiatry, Department of Psychiatry, 
      Psychotherapy and Preventive Medicine, LWL University Hospital, Ruhr-University 
      Bochum, Bochum, Germany.
FAU - Kostka, Michael
AU  - Kostka M
AD  - Department of Cell Morphology and Molecular Neurobiology, Ruhr-University Bochum, 
      Bochum, Germany.
FAU - Freund, Nadja
AU  - Freund N
AD  - Division of Experimental and Molecular Psychiatry, Department of Psychiatry, 
      Psychotherapy and Preventive Medicine, LWL University Hospital, Ruhr-University 
      Bochum, Bochum, Germany.
FAU - Juckel, Georg
AU  - Juckel G
AD  - Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, 
      Bochum, Germany.
FAU - Faissner, Andreas
AU  - Faissner A
AUID- ORCID: 0000-0002-2211-8259
AD  - Department of Cell Morphology and Molecular Neurobiology, Ruhr-University Bochum, 
      Bochum, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200828
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - Extracellular Matrix
MH  - Female
MH  - Hippocampus
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Neurons
MH  - *Poly I-C
MH  - Pregnancy
OTO - NOTNLM
OT  - extracellular matrix
OT  - multielectrode array
OT  - neuronal networks
OT  - perineuronal nets
OT  - schizophrenia
EDAT- 2020/08/07 06:00
MHDA- 2021/08/14 06:00
CRDT- 2020/08/07 06:00
PHST- 2020/07/08 00:00 [revised]
PHST- 2020/01/13 00:00 [received]
PHST- 2020/07/23 00:00 [accepted]
PHST- 2020/08/07 06:00 [pubmed]
PHST- 2021/08/14 06:00 [medline]
PHST- 2020/08/07 06:00 [entrez]
AID - 10.1111/ejn.14934 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2021 Jun;53(12):3920-3941. doi: 10.1111/ejn.14934. Epub 2020 Aug 
      28.