PMID- 32758104
OWN - NLM
STAT- MEDLINE
DCOM- 20210614
LR  - 20210614
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 63
IP  - 9
DP  - 2020 Sep
TI  - Integration of mandarin (Citrus reticulata) cytogenetic map with its genome 
      sequence.
PG  - 437-444
LID - 10.1139/gen-2020-0046 [doi]
AB  - Citrus is an extremely important genus in terms of world fruit production. 
      Despite its economic importance and the small genome sizes of its species (2n = 
      18, 1C = 430 +/- 68 Mbp), entire genomic assemblies have only recently become 
      available for some of its representatives. Together with the previous CMA/DAPI 
      banding and fluorescence in situ hybridization (FISH) in the group, these data 
      are important for understanding the complex relationships between its species and 
      for assisting breeding programs. To anchor genomic data with the cytogenetic map 
      of mandarin (Citrus reticulata), the parental species of several economically 
      important hybrids such as sweet orange and clementine, 18 BAC (bacterial 
      artificial chromosome) clones were used. Eleven clementine BACs were positioned 
      by BAC-FISH, doubling the number of chromosome markers so far available for 
      BAC-FISH in citrus. Additionally, six previously mapped BACs were end-sequenced, 
      allowing, together with one BAC previously sequenced, their assignment to 
      scaffolds and the subsequent integration of chromosomes and the genome assembly. 
      This study therefore established correlations between mandarin scaffolds and 
      chromosomes, allowing further structural genomic and comparative study with the 
      sweet orange genome, as well as insights into the chromosomal evolution of the 
      group.
FAU - Mendes, Sandra
AU  - Mendes S
AD  - Laboratorio de Citogenetica e Evolucao Vegetal, Departamento de Botanica, 
      Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
FAU - Regis, Thallita
AU  - Regis T
AD  - Laboratorio de Citogenetica e Evolucao Vegetal, Departamento de Botanica, 
      Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
FAU - Terol, Javier
AU  - Terol J
AD  - Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 
      Moncada, Valencia, Spain.
FAU - Soares Filho, Walter Dos Santos
AU  - Soares Filho WDS
AD  - Embrapa Mandioca e Fruticultura, Cruz das Almas, BA, Brazil.
FAU - Talon, Manuel
AU  - Talon M
AD  - Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 
      Moncada, Valencia, Spain.
FAU - Pedrosa-Harand, Andrea
AU  - Pedrosa-Harand A
AD  - Laboratorio de Citogenetica e Evolucao Vegetal, Departamento de Botanica, 
      Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20200806
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Base Sequence
MH  - Breeding
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant
MH  - Citrus/*genetics
MH  - Genetic Markers
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Sequence Analysis
MH  - *Whole Genome Sequencing
OTO - NOTNLM
OT  - bacterial artificial chromosome
OT  - cartographie comparee
OT  - chromosome bacterien artificiel
OT  - comparative mapping
OT  - fluorescent in situ hybridization
OT  - heterochromatin
OT  - hybridation in situ en fluorescence
OT  - heterochromatine
OT  - karyotype evolution
OT  - evolution du caryotype
EDAT- 2020/08/08 06:00
MHDA- 2021/06/16 06:00
CRDT- 2020/08/08 06:00
PHST- 2020/08/08 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2020/08/08 06:00 [entrez]
AID - 10.1139/gen-2020-0046 [doi]
PST - ppublish
SO  - Genome. 2020 Sep;63(9):437-444. doi: 10.1139/gen-2020-0046. Epub 2020 Aug 6.