PMID- 32762098 OWN - NLM STAT- MEDLINE DCOM- 20210621 LR - 20210621 IS - 1439-0531 (Electronic) IS - 0936-6768 (Linking) VI - 55 IP - 11 DP - 2020 Nov TI - Spatiotemporal heterogeneity of PPARgamma expression in porcine uteroplacenta for regulating of placental angiogenesis through VEGF-mediated signalling. PG - 1479-1489 LID - 10.1111/rda.13797 [doi] AB - Non-infectious prenatal mortality severely affects the porcine industry, with pathological placentation as a likely key reason. Previous studies have demonstrated that peroxisome proliferator-activated receptor gamma (PPARgamma) deficiency causes defects in the uteroplacental vasculature and induces embryonic losses in mice. However, its role in porcine placental angiogenesis remains unclear. In the present study, PPARgamma expression was investigated in porcine uteroplacental tissues at gestational day (GD) 25, GD40 and GD70 via quantitative polymerase chain reaction (qPCR), Western blot and immunohistochemistry (IHC). Moreover, the roles of PPARgamma in porcine placental angiogenesis were investigated using a cell model of porcine umbilical vein endothelial cells (PUVECs) to conduct proliferation, migration and tube formation assays in vitro and a mouse xenograft model to assess capillary formation in vivo. The results showed that PPARgamma was mainly located in the glandular epithelium, trophoblast, amniotic chorion epithelium and vascular endothelium, as indicated by the higher expression levels at GD25 and GD40 than at GD70 in endometrium and by higher expression levels at GD40 and GD70 than at GD25 in placenta. Moreover, PPARgamma expression was significantly downregulated in placenta with dead foetus. In PUVECs, knocking out PPARgamma significantly inhibited proliferation, migration and tube formation in vitro and inhibited capillary formation in mouse xenografts in vivo by blocking S-phase, promoting apoptosis and downregulating the angiogenic factors of VEGF and its receptors. Overall, the spatiotemporal heterogeneity of PPARgamma expression in porcine uteroplacental tissue suggests its vital role in endometrial remodelling and placental angiogenesis, and PPARgamma regulates placental angiogenesis through VEGF-mediated signalling. CI - (c) 2020 Wiley-VCH GmbH. FAU - Zhang, Juzuo AU - Zhang J AUID- ORCID: 0000-0002-0213-0365 AD - College of Biological and Food Engineering, 'Double First-Class' Applied Characteristic Discipline of Bioengineering in Hunan High Educational Institution, Huaihua University, Huaihua, China. AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Xue, Liqun AU - Xue L AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Nie, Ang AU - Nie A AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. AD - Huaihua Municipal Center of Animal Husbandry and Aquatic Products Bureau, Huaihua, China. FAU - Yang, Qing AU - Yang Q AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Peng, Xuan AU - Peng X AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Chen, Zhilong AU - Chen Z AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Yang, Lisha AU - Yang L AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Xie, Yang AU - Xie Y AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Yuan, Anwen AU - Yuan A AD - Department of Clinic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China. FAU - Xu, Junfei AU - Xu J AD - College of Biological and Food Engineering, 'Double First-Class' Applied Characteristic Discipline of Bioengineering in Hunan High Educational Institution, Huaihua University, Huaihua, China. LA - eng GR - 2018YFC1004103/The National Key Research and Development Program of China/ GR - The Foundation of Hunan Double First-rate Discipline Construction Projects/ GR - 81830041/The Natural Science Foundation of China/ GR - 31572591/The Natural Science Foundation of China/ GR - 31172377/The Natural Science Foundation of China/ GR - 2017M622806/The China Postdoctoral Foundation/ GR - JCYJ20180305164706355/The Shenzhen Basic Research Subject Project/ GR - JCYJ20170817143406822/The Shenzhen Basic Research Subject Project/ PT - Journal Article DEP - 20200901 PL - Germany TA - Reprod Domest Anim JT - Reproduction in domestic animals = Zuchthygiene JID - 9015668 RN - 0 (PPAR gamma) RN - 0 (Vascular Endothelial Growth Factor A) SB - IM MH - Animals MH - Capillaries/growth & development MH - Cell Line MH - Female MH - Heterografts MH - Mice, Inbred C57BL MH - Neovascularization, Physiologic MH - PPAR gamma/genetics/*metabolism MH - Placenta/*blood supply/metabolism MH - Pregnancy MH - Signal Transduction MH - Sus scrofa/*physiology MH - Uterus/metabolism MH - Vascular Endothelial Growth Factor A/metabolism OTO - NOTNLM OT - PPARgamma OT - VEGF signalling OT - pig OT - placental angiogenesis EDAT- 2020/08/08 06:00 MHDA- 2021/06/22 06:00 CRDT- 2020/08/08 06:00 PHST- 2020/05/16 00:00 [received] PHST- 2020/06/24 00:00 [revised] PHST- 2020/08/01 00:00 [accepted] PHST- 2020/08/08 06:00 [pubmed] PHST- 2021/06/22 06:00 [medline] PHST- 2020/08/08 06:00 [entrez] AID - 10.1111/rda.13797 [doi] PST - ppublish SO - Reprod Domest Anim. 2020 Nov;55(11):1479-1489. doi: 10.1111/rda.13797. Epub 2020 Sep 1.