PMID- 3276211
OWN - NLM
STAT- MEDLINE
DCOM- 19880225
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 130
IP  - 1
DP  - 1988 Jan
TI  - Glomerular immune deposits in experimental IgA nephropathy. A continuum of 
      circulating and in situ formed immune complexes.
PG  - 216-22
AB  - Studies were undertaken to elucidate the primary pathogenetic mechanisms 
      responsible for immunoglobulin (Ig) A immune complexes formation and glomerular 
      deposition in vivo. Monomeric (mIgA) and polymeric IgA (pIgA) anti-dinitrophenyl 
      (DNP) were purified from MOPC 315 myeloma. A DNP-conjugated Ficoll was used as an 
      antigen. For simulation of natural conditions of in vivo immune complex 
      formation, 131I-DNP-Ficoll and 125I-IgA were administered through the intravenous 
      and intraperitoneal routes, respectively. The kinetics half-life (t1/2) of the 
      antigen (2.9 hours) and either the pIgA (7.2 hours) or mIgA (6.3 hours) in the 
      experimental groups was not significantly different from the control. Glomerular 
      IgA immune deposits were detectable only in mice that received pIgA and 
      DNP-Ficoll. Plasma samples analyzed by gradient polyacrylamide gel 
      electrophoresis revealed formation of large- and intermediate-sized pIgA 
      complexes in circulation prior to glomerular deposition. Although mIgA failed to 
      interact with such complexes in the circulation, it did bind to the pIgA immune 
      deposits in the glomerulus. These results indicate that glomerular IgA immune 
      deposits evolve from the localization of preformed circulating pIgA complexes 
      that eventuates an in situ mIgA-mediated complex formation.
FAU - Chen, A
AU  - Chen A
AD  - Department of Pathology and Laboratory Medicine, University of Texas Health 
      Science Center at Houston.
FAU - Wong, S S
AU  - Wong SS
FAU - Rifai, A
AU  - Rifai A
LA  - eng
GR  - DK 32379/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Immunoglobulin A)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigen-Antibody Complex/*analysis
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Glomerulonephritis, IGA/immunology/*pathology
MH  - Immunoglobulin A/analysis
MH  - Kidney Glomerulus/*immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
PMC - PMC1880558
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
PMCR- 1988/07/01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PHST- 1988/07/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1988 Jan;130(1):216-22.