PMID- 32762286
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 29
IP  - 20
DP  - 2020 Oct 15
TI  - Immediate Intracoronary Delivery of Human Umbilical Cord Mesenchymal Stem Cells 
      Reduces Myocardial Injury by Regulating the Inflammatory Process Through 
      Cell-Cell Contact with T Lymphocytes.
PG  - 1331-1345
LID - 10.1089/scd.2019.0264 [doi]
AB  - Inflammatory response regulation is a mechanism through which human umbilical 
      cord mesenchymal stem cells (HUCMSCs) improve myocardial ischemia reperfusion 
      injury (IRI); however, the timing of HUCMSC delivery to achieve maximum 
      effectiveness is controversial. To investigate the effects of HUCMSC delivery on 
      the acute inflammatory stage of IRI, we transplanted HUCMSCs or HUCMSCs with 
      cyclosporin A (CsA) through the coronary artery simultaneously during ischemia 
      reperfusion in pigs. Ferumoxytol-labeled HUCMSCs (HUCMSC), HUCMSCs with 
      cyclosporin A (HUCMSC+CsA), and PBS (control) groups were investigated to 
      evaluate the homing of transplanted cells and changes in infarct features, 
      cardiac activity, and inflammatory response at three time points 
      post-transplantation. Animals were sacrificed 2 weeks later for histological 
      analysis of the hearts. We detected Prussian blue-dyed granules distributed 
      around T lymphocyte clusters in the infarct area in the HUCMSC group. Infarct 
      size and collagen deposition in the infarct area were lower in the HUCMSC group 
      than in the control and HUCMSC+CsA groups. Cardiac function was mildly impaired 
      in both the control and HUCMSC groups, whereas added CsA had a more severe 
      impact. The levels of proinflammatory markers were lower in the HUCMSC group than 
      in the control group at 24-h follow-up, and the difference was more significant 
      after adding CsA. There were more CD3(+) T lymphocytes and Foxp3(+) Tregs in the 
      HUCMSC group infarct area than in the other two groups. Proliferation rate of T 
      lymphocytes was higher in the HUCMSC group than in the other two groups. Indirect 
      co-culture experiments in vitro showed that MSCs promoted the generation of 
      CD4(+)CD25(+) Foxp3(+)Tregs through a paracrine mechanism. These results indicate 
      that immediate intracoronary delivery of HUCMSCs after ischemia reperfusion can 
      reduce acute myocardial IRI and promote myocardial repair, mainly through T 
      lymphocyte interactions to regulate the intense inflammatory response during the 
      acute inflammatory stage.
FAU - Liu, Chen
AU  - Liu C
AD  - Department of Cardiology, Affiliated Hospital of Yangzhou University, Yangzhou 
      City, People's Republic of China.
FAU - Kang, Li-Na
AU  - Kang LN
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Chen, Fu
AU  - Chen F
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Mu, Dan
AU  - Mu D
AD  - Department of Radiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Shen, Song
AU  - Shen S
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Wang, Kun
AU  - Wang K
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Hu, Jia-Xin
AU  - Hu JX
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Xie, Jun
AU  - Xie J
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
FAU - Xu, Biao
AU  - Xu B
AD  - Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, 
      Nanjing City, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200827
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Biomarkers)
RN  - 9007-34-5 (Collagen)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Animals
MH  - Biomarkers/blood
MH  - *Cell Communication
MH  - Cell Proliferation
MH  - Collagen/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Ferrosoferric Oxide/metabolism
MH  - Heart Ventricles/pathology/physiopathology
MH  - Inflammation/blood/*pathology
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocardial Infarction/pathology/physiopathology/therapy
MH  - Myocardium/*pathology
MH  - Paracrine Communication
MH  - Perfusion
MH  - Swine
MH  - Swine, Miniature
MH  - Systole
MH  - T-Lymphocytes/*immunology
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - T lymphocytes
OT  - cyclosporin A
OT  - human umbilical cord mesenchymal stem cells
OT  - inflammation
OT  - myocardial ischemia reperfusion injury
EDAT- 2020/08/09 06:00
MHDA- 2021/09/04 06:00
CRDT- 2020/08/09 06:00
PHST- 2020/08/09 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2020/08/09 06:00 [entrez]
AID - 10.1089/scd.2019.0264 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2020 Oct 15;29(20):1331-1345. doi: 10.1089/scd.2019.0264. Epub 
      2020 Aug 27.