PMID- 32764989
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 1178-6930 (Print)
IS  - 1178-6930 (Electronic)
IS  - 1178-6930 (Linking)
VI  - 13
DP  - 2020
TI  - Effect of Malus asiatica Nakai Leaf Flavonoids on the Prevention of Esophageal 
      Cancer in C57BL/6J Mice by Regulating the IL-17 Signaling Pathway.
PG  - 6987-6996
LID - 10.2147/OTT.S261033 [doi]
AB  - BACKGROUND: The aim of this study was to observe the preventive effect of 
      flavonoids extracted from Malus asiatica Nakai leaves (FMANL) on esophageal 
      cancer in mice, especially the ability of FMANL to regulate the interleukin 17 
      (IL-17) signaling pathway during this process. MATERIALS AND METHODS: The 
      C57BL/6J mice were treated with 4-nitroquinoline N-oxide (4NQO) to induce 
      esophageal cancer, and the visceral tissue index and the serum and esophageal 
      tissue indexes of mice were used to verify the effect of FMANL. RESULTS: The 
      experimental results showed that FMANL can effectively control the changes in 
      visceral tissue caused by esophageal cancer. FMANL could increase the cytokine 
      levels of interleukin 10 (IL-10), monocyte chemotactic protein 1 (MCP-1) and 
      decrease the cytokine levels of tumor necrosis factor alpha (TNF-alpha), interferon-gamma 
      (IFN-gamma), interleukin 6 (IL-6), and interleukin 12p70 (IL-12p70) in serum of mice 
      with esophageal cancer. FMANL could also reduce CD3(+), CD4(+), and CD8(+) and 
      enhance CD19(+) mouse peripheral blood lymphocytes. The results of qPCR and 
      Western blot analysis showed that FMANL could down-regulate the mRNA and protein 
      expression levels of IL-17, interleukin 23 (IL-23), interleukin 1 beta (IL-1beta), 
      chemokine (C-X-C) ligand 1 (CXCL1), chemokine (C-X-C) ligand 2 (CXCL2), S100 
      calcium-binding protein A8 (S100A8), S100 calcium-binding protein A9 (S100A9), 
      matrix metalloprotein 9 (MMP-9), and matrix metalloprotein 13 (MMP-1) in mice 
      with esophageal cancer. High-performance liquid chromatography (HPLC) detection 
      showed that FMANL contained 10 chemicals, including rutin, hyperoside, 
      isoquercitrin, dihydroquercetin, quercitrin, hesperidin, myricetin, baicalin, 
      neohesperidin dihydrochalcone, and quercetin. CONCLUSION: It could be concluded 
      that FMANL can effectively prevent experimentally induced esophageal cancer in 
      mice, and its effects might be obtained from 10 compounds present in FMANL.
CI  - (c) 2020 Jia et al.
FAU - Jia, Xiang-Bo
AU  - Jia XB
AD  - Department of Thoracic Surgery, Zhengzhou Key Laboratory of Surgical Treatment 
      for End-Stage Lung Diseases, Henan Provincial People's Hospital, People's 
      Hospital of Zhengzhou University, Zhengzhou 450003, Henan, People's Republic of 
      China.
FAU - Zhang, Quan
AU  - Zhang Q
AD  - Department of Thoracic Surgery, Zhengzhou Key Laboratory of Surgical Treatment 
      for End-Stage Lung Diseases, Henan Provincial People's Hospital, People's 
      Hospital of Zhengzhou University, Zhengzhou 450003, Henan, People's Republic of 
      China.
FAU - Xu, Lei
AU  - Xu L
AD  - Department of Thoracic Surgery, Zhengzhou Key Laboratory of Surgical Treatment 
      for End-Stage Lung Diseases, Henan Provincial People's Hospital, People's 
      Hospital of Zhengzhou University, Zhengzhou 450003, Henan, People's Republic of 
      China.
FAU - Yao, Wen-Jian
AU  - Yao WJ
AD  - Department of Thoracic Surgery, Zhengzhou Key Laboratory of Surgical Treatment 
      for End-Stage Lung Diseases, Henan Provincial People's Hospital, People's 
      Hospital of Zhengzhou University, Zhengzhou 450003, Henan, People's Republic of 
      China.
FAU - Wei, Li
AU  - Wei L
AD  - Department of Thoracic Surgery, Zhengzhou Key Laboratory of Surgical Treatment 
      for End-Stage Lung Diseases, Henan Provincial People's Hospital, People's 
      Hospital of Zhengzhou University, Zhengzhou 450003, Henan, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200717
PL  - New Zealand
TA  - Onco Targets Ther
JT  - OncoTargets and therapy
JID - 101514322
PMC - PMC7373410
OTO - NOTNLM
OT  - C57BL/6J mice
OT  - Malus asiatica Nakai leaves
OT  - esophageal cancer
OT  - flavonoid
OT  - mRNA IL-17 signaling pathway
COIS- The authors have no conflict of interest to declare.
EDAT- 2020/08/09 06:00
MHDA- 2020/08/09 06:01
PMCR- 2020/07/17
CRDT- 2020/08/09 06:00
PHST- 2020/05/03 00:00 [received]
PHST- 2020/06/26 00:00 [accepted]
PHST- 2020/08/09 06:00 [entrez]
PHST- 2020/08/09 06:00 [pubmed]
PHST- 2020/08/09 06:01 [medline]
PHST- 2020/07/17 00:00 [pmc-release]
AID - 261033 [pii]
AID - 10.2147/OTT.S261033 [doi]
PST - epublish
SO  - Onco Targets Ther. 2020 Jul 17;13:6987-6996. doi: 10.2147/OTT.S261033. 
      eCollection 2020.