PMID- 32766662
OWN - NLM
STAT- MEDLINE
DCOM- 20210426
LR  - 20210426
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 11
IP  - 8
DP  - 2020 Aug 19
TI  - Antimicrobial activity and mechanism of peptide CM4 against Pseudomonas 
      aeruginosa.
PG  - 7245-7254
LID - 10.1039/d0fo01031f [doi]
AB  - Antibacterial peptide CM4 (ABP-CM4) is a small cationic peptide with 
      broad-spectrum activities against bacteria, fungi and tumor cells and may 
      possibly be used as an antimicrobial agent. In this study, a C-terminal amidated 
      antibacterial peptide ABP-CM4 (ABP-CM4N) with the strongest antibacterial 
      activity was obtained through screening the antibacterial activities of ABP-CM4 
      with different modifications. The minimal inhibitory concentration of ABP-CM4N 
      was 8 muM against P. aeruginosa (ATCC 27853) which was lower than that of ABP-CM4 
      (16 muM). The strengthened antimicrobial activity of ABP-CM4N may be associated 
      with the increased membrane binding capacity, being two times that of ABP-CM4 (p 
      < 0.001). The antibacterial mechanism of ABP-CM4N to Pseudomonas aeruginosa was 
      examined by means of cell membrane integrity analysiss, the intracellular 
      ultrastructure change observation and E. coli genomic DNA binding assay. It was 
      found that ABP-CM4N had the same antimicrobial mechanism as ABP-CM4, and the aim 
      of the antimicrobial mechanism was mainly to destroy the cell membrane which 
      caused nucleic acid or protein leakage, and secondly to interact with E. coli 
      genomic DNA after penetrating the cell membrane. Furthermore, in vitro ABP-CM4N 
      showed a better bacteriostatic activity in meats, with the treated samples 
      showing two to three times less positive colonies than ABP-CM4.
FAU - Li, Jian-Feng
AU  - Li JF
AUID- ORCID: 0000-0003-4763-7968
AD  - Institute of Aging Research, School of Medicine, Hangzhou Normal University, 
      Hangzhou, Zhejiang Province 311121, China. lijianfeng@hznu.edu.cn.
FAU - Zhang, Jia-Xin
AU  - Zhang JX
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing 210046, China.
FAU - Li, Guo
AU  - Li G
AD  - Department of Biochemistry and Molecular Biology and Key Laboratory of Molecular 
      Biology, School of Basic Medicine and Life Sciences, Hainan Medical College, 
      Haikou, 571199, China.
FAU - Xu, Yan-Yan
AU  - Xu YY
AD  - Institute of Aging Research, School of Medicine, Hangzhou Normal University, 
      Hangzhou, Zhejiang Province 311121, China. lijianfeng@hznu.edu.cn.
FAU - Lu, Kai
AU  - Lu K
AD  - School of Medicine, Wenzhou Medical College, Wenzhou, Zhejiang Province 325035, 
      China.
FAU - Wang, Zhi-Guo
AU  - Wang ZG
AUID- ORCID: 0000-0001-8732-4451
AD  - Institute of Aging Research, School of Medicine, Hangzhou Normal University, 
      Hangzhou, Zhejiang Province 311121, China. lijianfeng@hznu.edu.cn.
FAU - Liu, Jun-Ping
AU  - Liu JP
AD  - Institute of Aging Research, School of Medicine, Hangzhou Normal University, 
      Hangzhou, Zhejiang Province 311121, China. lijianfeng@hznu.edu.cn.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Anti-Infective Agents/*pharmacology
MH  - Antimicrobial Cationic Peptides/*pharmacology
MH  - Cell Membrane/drug effects/metabolism
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Escherichia coli/drug effects/genetics
MH  - Food Contamination/prevention & control
MH  - Food Microbiology
MH  - Meat/microbiology
MH  - Microbial Sensitivity Tests
MH  - Protein Conformation
MH  - Pseudomonas aeruginosa/*drug effects/genetics/metabolism
EDAT- 2020/08/09 06:00
MHDA- 2021/04/27 06:00
CRDT- 2020/08/09 06:00
PHST- 2020/08/09 06:00 [pubmed]
PHST- 2021/04/27 06:00 [medline]
PHST- 2020/08/09 06:00 [entrez]
AID - 10.1039/d0fo01031f [doi]
PST - ppublish
SO  - Food Funct. 2020 Aug 19;11(8):7245-7254. doi: 10.1039/d0fo01031f.