PMID- 32767851
OWN - NLM
STAT- MEDLINE
DCOM- 20210119
LR  - 20210119
IS  - 1601-6343 (Electronic)
IS  - 1601-6335 (Linking)
VI  - 24
IP  - 1
DP  - 2021 Feb
TI  - Involvement of astrocytes activation in orofacial hyperalgesia induced by 
      experimental tooth movement.
PG  - 147-154
LID - 10.1111/ocr.12418 [doi]
AB  - OBJECTIVE: The study aimed to investigate the involvement of astrocytes in the 
      medullary dorsal horn (MDH) in the orofacial hyperalgesia induced by experimental 
      tooth movement (ETM) and related mechanism. MATERIALS AND METHODS: Experimental 
      tooth movement was produced with nickel-titanium alloy closed-coil spring fixed 
      between the left maxillary first molar and the left upper incisor. Fluorocitrate 
      was administrated through medullary subarachnoid at 3 days after ETM. Pressure 
      pain threshold (PPT) in masseter cutaneous area was measured. The expression of 
      glial fibrillary acidic protein (GFAP) and c-Fos in MDH was measured using 
      immunofluoroscence staining. The expression of interleukin-1beta (IL-1beta) and 
      phosphorylated N-methyl-D-aspartic acid (NMDA) receptor subunit NR1 (p-NR1) was 
      measured with Western blotting. RESULTS: Experimental tooth movement-induced 
      orofacial hyperalgesia from 1 to 9 days as the PPT was significantly reduced 
      (P < .05). Immunofluoroscence staining showed that the expression of c-Fos in MDH 
      was dramatically upregulated at 1 day and 3 days after ETM, while GFAP expression 
      with both immunofluoroscence staining and Western blotting was significantly 
      enhanced at 3 days and 7 days after ETM. Western blotting analysis indicated that 
      the expression of IL-1beta and p-NR1 in MDH was significantly enhanced at 3 days 
      after ETM. Furthermore, we found that fluorocitrate administration at 3 days 
      after ETM could markedly suppress the expression of c-Fos, GFAP, IL-1beta and p-NR1 
      and attenuate the reduction of PPT induced by ETM. CONCLUSION: Astrocyte 
      activation in MDH is involved in the mechanical hyperalgesia, and the subsequent 
      upregulated IL-1beta and overexpression of p-NR1 may participate in this process.
CI  - (c) 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Li, Cui-Xia
AU  - Li CX
AD  - State Key Laboratory of Military Stomatology & National Clinical Research Center 
      for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, 
      Department of Orthodontics, School of Stomatology, The Fourth Military Medical 
      University, Xi'an, China.
FAU - Liu, Jia
AU  - Liu J
AD  - State Key Laboratory of Military Stomatology & National Clinical Research Center 
      for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, 
      Department of Orthodontics, School of Stomatology, The Fourth Military Medical 
      University, Xi'an, China.
FAU - Zhou, Kai-Xiang
AU  - Zhou KX
AD  - Department of Anatomy, Histology and Embryology, K.K. Leung Brain Research 
      Centre, Fourth Military Medical University, Xi'an, China.
FAU - Zhao, Wen-Jun
AU  - Zhao WJ
AD  - Department of Anatomy, Histology and Embryology, K.K. Leung Brain Research 
      Centre, Fourth Military Medical University, Xi'an, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - State Key Laboratory of Military Stomatology & National Clinical Research Center 
      for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, 
      Department of Orthodontics, School of Stomatology, The Fourth Military Medical 
      University, Xi'an, China.
FAU - Jin, Zuo-Lin
AU  - Jin ZL
AUID- ORCID: 0000-0001-7871-110X
AD  - State Key Laboratory of Military Stomatology & National Clinical Research Center 
      for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, 
      Department of Orthodontics, School of Stomatology, The Fourth Military Medical 
      University, Xi'an, China.
FAU - Gu, Ze-Xu
AU  - Gu ZX
AD  - State Key Laboratory of Military Stomatology & National Clinical Research Center 
      for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, 
      Department of Orthodontics, School of Stomatology, The Fourth Military Medical 
      University, Xi'an, China.
LA  - eng
GR  - 81571000/National Nature Science Foundation of China/
GR  - 81970960/National Nature Science Foundation of China/
GR  - 81701002/National Nature Science Foundation of China/
GR  - ZZQP003/General Equipment Department of People's Liberation Army Youth Project/
PT  - Journal Article
DEP - 20200914
PL  - England
TA  - Orthod Craniofac Res
JT  - Orthodontics & craniofacial research
JID - 101144387
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - *Astrocytes
MH  - Glial Fibrillary Acidic Protein
MH  - *Hyperalgesia
MH  - Pain Threshold
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - astrocytes
OT  - experimental tooth movement
OT  - fluorocitrate
OT  - medullary dorsal horn
OT  - orofacial hyperalgesia
EDAT- 2020/08/09 06:00
MHDA- 2021/01/20 06:00
CRDT- 2020/08/09 06:00
PHST- 2020/05/03 00:00 [received]
PHST- 2020/07/28 00:00 [revised]
PHST- 2020/08/03 00:00 [accepted]
PHST- 2020/08/09 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
PHST- 2020/08/09 06:00 [entrez]
AID - 10.1111/ocr.12418 [doi]
PST - ppublish
SO  - Orthod Craniofac Res. 2021 Feb;24(1):147-154. doi: 10.1111/ocr.12418. Epub 2020 
      Sep 14.