PMID- 32768983 OWN - NLM STAT- MEDLINE DCOM- 20210617 LR - 20210617 IS - 1873-4367 (Electronic) IS - 0927-7765 (Linking) VI - 196 DP - 2020 Dec TI - Dopamine-functionalized hyaluronic acid microspheres for effective capture of CD44-overexpressing circulating tumor cells. PG - 111281 LID - S0927-7765(20)30637-8 [pii] LID - 10.1016/j.colsurfb.2020.111281 [doi] AB - As one of the biomarkers of liquid biopsy, circulating tumor cells (CTCs) provides important clinical information for cancer diagnosis. However, accurate separation and identification of CTCs remains a great deal of challenge. In present work, we developed novel dopamine-functionalized hyaluronic acid microspheres (HA-DA microspheres) to capture CD44-overexpressing CTCs. The dopamine was grafted onto the hyaluronic acid chain, which was polymerized and cross-linked by oxidation of the catechol groups. Afterwards, a facile microfluidic chip was designed and developed to fabricate the HA-DA microspheres with a diameter of about 45 mum. Our results showed that the CD44+ cells (i.e., HeLa, HepG2, A549, MCF-7 and DU-145 cells) could be selectively captured. Then a double-layer microfluidic filter (DLMF) was fabricated for dynamic isolation and detection of CTCs in blood samples. Many slit openings with 15 mum in height were designed to allow white blood cells to clear away, while the microspheres with CTCs were intercepted in the DLMF, which achieved effective separation of CTCs from blood cells. The approach exhibited high capture efficiency even at the cell density as low as 10 cells/mL. We believe the DLMF integrated with HA-DA microspheres could be a promising approach for isolation and detection of CD44-overexpressing CTCs, which is useful for prognosis and early metastasis of cancer patients. CI - Copyright (c) 2020 Elsevier B.V. All rights reserved. FAU - Li, Xiuping AU - Li X AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. FAU - Cui, Tianyu AU - Cui T AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. FAU - Zhang, Wenxian AU - Zhang W AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. FAU - Zhai, Ziran AU - Zhai Z AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. FAU - Wu, Feixuan AU - Wu F AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. FAU - Zhang, Yuwei AU - Zhang Y AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. FAU - Yang, Mengsu AU - Yang M AD - Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People's Republic of China. FAU - Zhong, Wenying AU - Zhong W AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China. Electronic address: wyzhong@cpu.edu.cn. FAU - Yue, Wanqing AU - Yue W AD - Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, People's Republic of China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, People's Republic of China. Electronic address: yuewq@cpu.edu.cn. LA - eng PT - Journal Article DEP - 20200729 PL - Netherlands TA - Colloids Surf B Biointerfaces JT - Colloids and surfaces. B, Biointerfaces JID - 9315133 RN - 0 (CD44 protein, human) RN - 0 (Hyaluronan Receptors) RN - 9004-61-9 (Hyaluronic Acid) RN - VTD58H1Z2X (Dopamine) SB - IM MH - Cell Count MH - Cell Line, Tumor MH - Cell Separation MH - Dopamine MH - Humans MH - Hyaluronan Receptors MH - Hyaluronic Acid MH - Microspheres MH - *Neoplastic Cells, Circulating OTO - NOTNLM OT - CD44 OT - Circulating tumor cells OT - Hyaluronic acid OT - Microspheres OT - Micro fl uidics EDAT- 2020/08/10 06:00 MHDA- 2021/06/22 06:00 CRDT- 2020/08/10 06:00 PHST- 2020/03/04 00:00 [received] PHST- 2020/06/19 00:00 [revised] PHST- 2020/07/25 00:00 [accepted] PHST- 2020/08/10 06:00 [pubmed] PHST- 2021/06/22 06:00 [medline] PHST- 2020/08/10 06:00 [entrez] AID - S0927-7765(20)30637-8 [pii] AID - 10.1016/j.colsurfb.2020.111281 [doi] PST - ppublish SO - Colloids Surf B Biointerfaces. 2020 Dec;196:111281. doi: 10.1016/j.colsurfb.2020.111281. Epub 2020 Jul 29.