PMID- 32771793
OWN - NLM
STAT- MEDLINE
DCOM- 20201020
LR  - 20201020
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 747
DP  - 2020 Dec 10
TI  - Composition and diversity of soil microbial communities in the alpine wetland and 
      alpine forest ecosystems on the Tibetan Plateau.
PG  - 141358
LID - S0048-9697(20)34887-7 [pii]
LID - 10.1016/j.scitotenv.2020.141358 [doi]
AB  - While the composition and diversity of soil microbial communities play a central 
      and essential role in biogeochemical cycling of nutrients, they are known to be 
      shaped by the physical and chemical properties of soils and various environmental 
      factors. This study investigated the composition and diversity of microbial 
      communities in 48 samples of seasonally frozen soils collected from 16 sites in 
      an alpine wetland region (Lhasa River basin) and an alpine forest region (Nyang 
      River basin) on the Tibetan Plateau using high-throughput sequencing that 
      targeted the V3-V4 region of 16S rRNA gene. The dominant soil microbial phyla 
      included Proteobacteria, Acidobacteria, and Actinobacteria in the alpine wetland 
      and alpine forest ecosystems, and no significant difference was observed for 
      their microbial composition. Linear discriminant analysis Effect Size (LEfSe) 
      analysis showed that significant enrichment of Hymenobacteraceae and Cytophagales 
      (belonging to Bacteroidetes) existed in the alpine wetland soils, while the 
      alpine forest soils were enriched with Alphaproteobacteria (belonging to 
      Proteobacteria), suggesting that these species could be potential biomarkers for 
      alpine wetland and alpine forest ecosystems. Results of redundancy analysis (RDA) 
      suggest that the microbial community diversity and abundance in the seasonally 
      frozen soils on the Tibetan Plateau were mainly related to the total potassium in 
      the alpine wetland ecosystem, and available potassium and soil moisture in the 
      alpine forest ecosystem, respectively. In addition, function prediction analysis 
      by Tax4Fun revealed the existence of potential functional pathways involved in 
      human diseases in all soil samples. These results provide insights on the 
      structure and function of soil microbial communities in the alpine wetland and 
      alpine forest ecosystems on the Tibetan Plateau, while the potential risk to 
      human health from the pathogenic microbes in the seasonally frozen soils deserves 
      attention.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Wang, Xiaojie
AU  - Wang X
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of 
      Chinese Academy of Sciences, Beijing 100049, China; MOE Key Laboratory for Earth 
      Surface Processes, College of Urban and Environmental Sciences, Peking 
      University, Beijing 100871, China.
FAU - Zhang, Zhichao
AU  - Zhang Z
AD  - MOE Key Laboratory for Earth Surface Processes, College of Urban and 
      Environmental Sciences, Peking University, Beijing 100871, China.
FAU - Yu, Zhiqiang
AU  - Yu Z
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
FAU - Shen, Guofeng
AU  - Shen G
AD  - MOE Key Laboratory for Earth Surface Processes, College of Urban and 
      Environmental Sciences, Peking University, Beijing 100871, China.
FAU - Cheng, Hefa
AU  - Cheng H
AD  - MOE Key Laboratory for Earth Surface Processes, College of Urban and 
      Environmental Sciences, Peking University, Beijing 100871, China. Electronic 
      address: hefac@umich.edu.
FAU - Tao, Shu
AU  - Tao S
AD  - MOE Key Laboratory for Earth Surface Processes, College of Urban and 
      Environmental Sciences, Peking University, Beijing 100871, China.
LA  - eng
PT  - Journal Article
DEP - 20200729
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
SB  - IM
MH  - Forests
MH  - *Microbiota
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil
MH  - Soil Microbiology
MH  - Tibet
MH  - Wetlands
OTO - NOTNLM
OT  - Functional pathway
OT  - Microbial community composition
OT  - Seasonally frozen soils
OT  - Soil microbial diversity
OT  - Spatial variability
OT  - Tibetan Plateau
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/08/11 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/08/11 06:00
PHST- 2020/05/08 00:00 [received]
PHST- 2020/07/27 00:00 [revised]
PHST- 2020/07/27 00:00 [accepted]
PHST- 2020/08/11 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/08/11 06:00 [entrez]
AID - S0048-9697(20)34887-7 [pii]
AID - 10.1016/j.scitotenv.2020.141358 [doi]
PST - ppublish
SO  - Sci Total Environ. 2020 Dec 10;747:141358. doi: 10.1016/j.scitotenv.2020.141358. 
      Epub 2020 Jul 29.