PMID- 3277745
OWN - NLM
STAT- MEDLINE
DCOM- 19880328
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 34
IP  - 2
DP  - 1988 Feb
TI  - "Sandwich"-type immunoassay of carcinoembryonic antigen in patients receiving 
      murine monoclonal antibodies for diagnosis and therapy.
PG  - 261-4
AB  - Measurements of carcinoembryonic antigen (CEA) in blood increased dramatically in 
      some patients who were receiving injections of monoclonal antibody. CEA titers 
      were measured with a monoclonal antibody-based double-determinant enzyme 
      immunoassay in which untreated plasma specimens were diluted with an equal volume 
      of buffer containing mouse serum. Increasing CEA titers were accompanied by the 
      appearance and coincident increase in titers of human antibody against mouse Ig 
      (HAMA). Adsorption of these sera with solid-phase anti-human IgG or Protein A 
      restored antigen titers to pretreatment values; evidently the serum factor 
      eliciting false-positive CEA titers was most probably HAMA. Neither addition of 
      undiluted mouse serum to the assay mixture nor pretreatment by heating plasma 
      specimens to 70 degrees C effectively abolished HAMA interference. By contrast, 
      protein precipitation with polyethylene glycol (130 g/L) or heating plasma 
      samples to 90 degrees C eliminated false-positive titers caused by HAMA, but did 
      not reduce authentic CEA titers.
FAU - Primus, F J
AU  - Primus FJ
AD  - Center for Molecular Medicine and Immunology, University of Medicine and 
      Dentistry of New Jersey, Newark 07103.
FAU - Kelley, E A
AU  - Kelley EA
FAU - Hansen, H J
AU  - Hansen HJ
FAU - Goldenberg, D M
AU  - Goldenberg DM
LA  - eng
GR  - CA 39841/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Immunoglobulin G)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*analysis
MH  - Antibodies, Monoclonal/immunology/*therapeutic use
MH  - Carcinoembryonic Antigen/*analysis
MH  - Chemical Precipitation
MH  - Colonic Neoplasms/*immunology/therapy
MH  - False Positive Reactions
MH  - Hot Temperature
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Immunoglobulin G/immunology
MH  - Immunosorbent Techniques
MH  - Mice
MH  - Polyethylene Glycols
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1988 Feb;34(2):261-4.