PMID- 32778239
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 122
IP  - 6
DP  - 2020 Sep
TI  - Endoplasmic reticulum stress mediates osteocyte death under oxygen-glucose 
      deprivation in vitro.
PG  - 151577
LID - S0065-1281(20)30076-3 [pii]
LID - 10.1016/j.acthis.2020.151577 [doi]
AB  - As a vascularized organ, bone is known to be susceptible to ischemia. Ischemic 
      osteonecrosis or skeletal unloading lead to ischemia in bone microenvironment 
      that causes osteocytes to suffer hypoxia and nutrition deprivation. OBJECTIVE: To 
      explore the effects of Oxygen-glucose deprivation (OGD) on osteocytes and the 
      potential mechanism. METHODS: OGD model was established in cultured MLO-Y4 cell. 
      Cell damage, intracellular oxidative stress and cell apoptosis were detected at 
      different OGD times (0, 2, 4, 8, 12, 24 h), and the changes in endoplasmic 
      reticulum (ER) stress-related indicators were observed. Furthermore, cells were 
      treated with 4-phenylbutyrate sodium (4-PBA) to inhibit ER stress, and cell 
      damage and oxidative stress level were detected. RESULTS: The cell viability 
      under OGD exhibited a significantly reduced in a time-dependent manner, and the 
      level of intracellular reactive oxygen species (ROS) were increased, cell 
      apoptosis and ER stress was induced. Inhibition of ER stress can reduce cell 
      death and intracellular ROS levels. CONCLUSION: Our study demonstrated that ER 
      stress regulates OGD-induced apoptotic cell death in MLO-Y4 cells via 
      intracellular ROS.
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Wang, Yizhong
AU  - Wang Y
AD  - Department of Orthopaedics, Xiangyang No.1 People' Hospital, Hubei University of 
      Medicine, Xiangyang, 441000, China; Department of Orthopedic Surgery, Zhongnan 
      Hospital of Wuhan University, Wuhan, 430071, China. Electronic address: 
      yizhong0520@163.com.
FAU - Han, Bing
AU  - Han B
AD  - Department of Orthopaedics, Xiangyang No.1 People' Hospital, Hubei University of 
      Medicine, Xiangyang, 441000, China.
FAU - Ding, Junhui
AU  - Ding J
AD  - Department of Orthopaedics, Xiangyang No.1 People' Hospital, Hubei University of 
      Medicine, Xiangyang, 441000, China.
FAU - Qiu, Chen
AU  - Qiu C
AD  - Department of Orthopaedics, Xiangyang No.1 People' Hospital, Hubei University of 
      Medicine, Xiangyang, 441000, China.
FAU - Wang, Wenbo
AU  - Wang W
AD  - Postgraduate Support Base of Xiangyang First People' Hospital of Jinzhou Medical 
      University, Xiangyang, 441000, China.
LA  - eng
PT  - Journal Article
DEP - 20200624
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Reactive Oxygen Species)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Blotting, Western
MH  - Cell Death/physiology
MH  - Cell Survival/genetics/physiology
MH  - Endoplasmic Reticulum Stress/genetics/*physiology
MH  - Glucose/*metabolism
MH  - Mice
MH  - Osteocytes/*cytology/*metabolism
MH  - Oxidative Stress/genetics/physiology
MH  - Oxygen/*metabolism
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Axidative stress
OT  - Endoplasmic reticulum stress
OT  - Osteocyte
OT  - Osteonecrosis
COIS- Declaration of Competing Interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/08/12 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/08/12 06:00
PHST- 2020/04/01 00:00 [received]
PHST- 2020/06/06 00:00 [revised]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/08/12 06:00 [entrez]
PHST- 2020/08/12 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
AID - S0065-1281(20)30076-3 [pii]
AID - 10.1016/j.acthis.2020.151577 [doi]
PST - ppublish
SO  - Acta Histochem. 2020 Sep;122(6):151577. doi: 10.1016/j.acthis.2020.151577. Epub 
      2020 Jun 24.