PMID- 32779889
OWN - NLM
STAT- MEDLINE
DCOM- 20210512
LR  - 20210512
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 24
IP  - 18
DP  - 2020 Sep
TI  - Malaria in pregnancy regulates P-glycoprotein (P-gp/Abcb1a) and ABCA1 efflux 
      transporters in the Mouse Visceral Yolk Sac.
PG  - 10636-10647
LID - 10.1111/jcmm.15682 [doi]
AB  - Malaria in pregnancy (MiP) induces intrauterine growth restriction (IUGR) and 
      preterm labour (PTL). However, its effects on yolk sac morphology and function 
      are largely unexplored. We hypothesized that MiP modifies yolk sac morphology and 
      efflux transport potential by modulating ABC efflux transporters. C57BL/6 mice 
      injected with Plasmodium berghei ANKA (5 x 10(5) infected erythrocytes) at 
      gestational day (GD) 13.5 were subjected to yolk sac membrane harvesting at GD 
      18.5 for histology, qPCR and immunohistochemistry. MiP did not alter the 
      volumetric proportion of the yolk sac's histological components. However, it 
      increased levels of Abcb1a mRNA (encoding P-glycoprotein) and macrophage 
      migration inhibitory factor (Mif chemokine), while decreasing Abcg1 (P < 0.05); 
      without altering Abca1, Abcb1b, Abcg2, Snat1, Snat2, interleukin (Il)-1beta and C-C 
      Motif chemokine ligand 2 (Ccl2). Transcripts of Il-6, chemokine (C-X-C motif) 
      ligand 1 (Cxcl1), Glut1 and Snat4 were not detectible. ABCA1, ABCG1, breast 
      cancer resistance protein (BCRP) and P-gp were primarily immunolocalized to the 
      cell membranes and cytoplasm of endodermic epithelium but also in the mesothelium 
      and in the endothelium of mesodermic blood vessels. Intensity of P-gp labelling 
      was stronger in both endodermic epithelium and mesothelium, whereas ABCA1 
      labelling increased in the endothelium of the mesodermic blood vessels. The 
      presence of ABC transporters in the yolk sac wall suggests that this fetal 
      membrane acts as an important protective gestational barrier. Changes in ABCA1 
      and P-gp in MiP may alter the biodistribution of toxic substances, xenobiotics, 
      nutrients and immunological factors within the fetal compartment and participate 
      in the pathogenesis of malaria-induced IUGR and PTL.
CI  - (c) 2020 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Martinelli, Lilian M
AU  - Martinelli LM
AD  - Department of Morphology, Institute of Biological Sciences, Federal University of 
      Minas Gerais, Belo Horizonte, Brazil.
FAU - Fontes, Klaus N
AU  - Fontes KN
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Reginatto, Mila W
AU  - Reginatto MW
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Andrade, Cherley B V
AU  - Andrade CBV
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Monteiro, Victoria R S
AU  - Monteiro VRS
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Gomes, Hanailly R
AU  - Gomes HR
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Silva-Filho, Joao L
AU  - Silva-Filho JL
AD  - Laboratory of Immunology and Biochemistry of Parasitic Diseases, Institute of 
      Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de 
      Janeiro, Brazil.
FAU - Pinheiro, Ana A S
AU  - Pinheiro AAS
AD  - Laboratory of Immunology and Biochemistry of Parasitic Diseases, Institute of 
      Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de 
      Janeiro, Brazil.
FAU - Vago, Annamaria R
AU  - Vago AR
AD  - Department of Morphology, Institute of Biological Sciences, Federal University of 
      Minas Gerais, Belo Horizonte, Brazil.
FAU - Almeida, Fernanda R C L
AU  - Almeida FRCL
AD  - Department of Morphology, Institute of Biological Sciences, Federal University of 
      Minas Gerais, Belo Horizonte, Brazil.
FAU - Bloise, Flavia F
AU  - Bloise FF
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Matthews, Stephen G
AU  - Matthews SG
AD  - Department of Physiology, University of Toronto, Toronto, ON, Canada.
AD  - Department of Obstetrics and Gynecology, University of Toronto, Toronto, ON, 
      Canada.
AD  - Department of Medicine, University of Toronto, Toronto, ON, Canada.
AD  - Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, 
      Canada.
FAU - Ortiga-Carvalho, Tania M
AU  - Ortiga-Carvalho TM
AUID- ORCID: 0000-0002-0225-1132
AD  - Laboratory of Translational Endocrinology, Institute of Biophysics Carlos Chagas 
      Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Bloise, Enrrico
AU  - Bloise E
AUID- ORCID: 0000-0003-0275-8686
AD  - Department of Morphology, Institute of Biological Sciences, Federal University of 
      Minas Gerais, Belo Horizonte, Brazil.
LA  - eng
GR  - OPP1107597/GATES/Bill & Melinda Gates Foundation/United States
GR  - Foundation-148368/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200811
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (ABCA1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9EI49ZU76O (multidrug resistance protein 3)
SB  - IM
MH  - ATP Binding Cassette Transporter 1/*biosynthesis/genetics
MH  - ATP Binding Cassette Transporter, Subfamily B/*biosynthesis/genetics
MH  - Animals
MH  - Biological Transport
MH  - Cytokines/biosynthesis/genetics
MH  - Female
MH  - Fetal Growth Retardation/etiology
MH  - *Gene Expression Regulation
MH  - Inflammation
MH  - Malaria/complications/genetics/*metabolism
MH  - Membrane Transport Proteins/biosynthesis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Organ Size
MH  - Plasmodium berghei
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/genetics/*metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Yolk Sac/*metabolism/ultrastructure
PMC - PMC7521277
OTO - NOTNLM
OT  - Plasmodium berghei ANKA
OT  - ABCA1
OT  - ABCG1
OT  - Breast Cancer Resistance Protein (BCRP)
OT  - P-glycoprotein (P-gp)
OT  - malaria in pregnancy (MiP)
OT  - yolk sac
COIS- The authors confirm that there are no conflicts of interest.
EDAT- 2020/08/12 06:00
MHDA- 2021/05/13 06:00
PMCR- 2020/09/01
CRDT- 2020/08/12 06:00
PHST- 2019/12/28 00:00 [received]
PHST- 2020/06/09 00:00 [revised]
PHST- 2020/07/09 00:00 [accepted]
PHST- 2020/08/12 06:00 [pubmed]
PHST- 2021/05/13 06:00 [medline]
PHST- 2020/08/12 06:00 [entrez]
PHST- 2020/09/01 00:00 [pmc-release]
AID - JCMM15682 [pii]
AID - 10.1111/jcmm.15682 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2020 Sep;24(18):10636-10647. doi: 10.1111/jcmm.15682. Epub 2020 
      Aug 11.