PMID- 32780718
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 2501-062X (Electronic)
IS  - 1220-4749 (Linking)
VI  - 58
IP  - 4
DP  - 2020 Dec 1
TI  - Role of urinary podocalyxin in early diagnosis of diabetic nephropathy.
PG  - 233-241
LID - 10.2478/rjim-2020-0023 [doi]
AB  - Introduction. Podocyte injury has been reported as an early feature of DN 
      therefore, the assessment of podocyte injury can be accomplished by estimation of 
      podocalyxin in urine. This study aimed to estimate the urinary podocalyxin levels 
      and to determine the sensitivity and specificity of this biomarker for early 
      detection of DN.Materials and methods. A total of 90 patients with type 2 
      diabetes mellitus (T2DM) were included in this cross-sectional study. Sixty of 
      them were without diagnosed DN, and 30 with diagnosed DN. A control group 
      consisted of 30 healthy subjects. All patients with T2DM were divided into three 
      subgroups according to urinary microalbumin/creatinine ratio (UM/CR): 
      normoalbuminuric, microalbuminuric and macroalbuminuric patients. Urine samples, 
      were used for measurement of podocalyxin by ELISA, creatinine and microalbumin. 
      Fasting venous blood samples was collected for biochemical analyses.Results. The 
      levels of urinary podocalyxin (u-PDX) were higher in patients with T2DM compared 
      to control subjects and a statistically significant difference among studied 
      subgroups regarding u-PDX was found (p < 0.05). Levels of u-PDX are increasing 
      gradually with the degree of DN (p < 0.029). u-PDX levels were positively 
      correlated with UM/CR (r = 0.227, p = 0.002). A cut-off level of 43.8 ng/ml u-PDX 
      showed 73.3% sensitivity and 93.3% specificity to detect DN in early stage. A 
      cut-off level of 30 mg/g UM/CR showed 41.5% sensitivity and 90% specificity in 
      predicting DN. u-PDX was elevated in 48,2% of normoalbuminuric 
      patients.Conclusion. Urinary podocalyxin be useful and more sensitive and 
      specific marker in early detection of DN than microalbuminuria.
CI  - (c) 2020 Irena Kostovska et al., published by Sciendo.
FAU - Kostovska, Irena
AU  - Kostovska I
AUID- ORCID: 0000-0003-0971-6710
AD  - Department of Medical Biochemistry, Faculty of Medicine, Str. 50 Divizija 6, 
      1000, Skopje, North Macedonia.
FAU - Trajkovska, Katerina Tosheska
AU  - Trajkovska KT
AD  - Department of Medical Biochemistry, Faculty of Medicine, Str. 50 Divizija 6, 
      1000, Skopje, North Macedonia.
FAU - Cekovska, Svetlana
AU  - Cekovska S
AD  - Department of Medical Biochemistry, Faculty of Medicine, Str. 50 Divizija 6, 
      1000, Skopje, North Macedonia.
FAU - Topuzovska, Sonja
AU  - Topuzovska S
AD  - Department of Medical Biochemistry, Faculty of Medicine, Str. 50 Divizija 6, 
      1000, Skopje, North Macedonia.
FAU - Kavrakova, Julijana Brezovska
AU  - Kavrakova JB
AD  - Department of Medical Biochemistry, Faculty of Medicine, Str. 50 Divizija 6, 
      1000, Skopje, North Macedonia.
FAU - Spasovski, Goce
AU  - Spasovski G
AD  - University Clinic of Nephrology, Faculty of Medicine, Str. Mother Teresa 17, 
      1000, Skopje, North Macedonia.
FAU - Kostovski, Ognen
AU  - Kostovski O
AD  - University Clinic of Abdominal Surgery, Faculty of Medicine, Str. Mother Teresa 
      17, 1000, Skopje, North Macedonia.
FAU - Labudovic, Danica
AU  - Labudovic D
AD  - Department of Medical Biochemistry, Faculty of Medicine, Str. 50 Divizija 6, 
      1000, Skopje, North Macedonia.
LA  - eng
PT  - Journal Article
DEP - 20201217
PL  - Germany
TA  - Rom J Intern Med
JT  - Romanian journal of internal medicine = Revue roumaine de medecine interne
JID - 9304507
RN  - 0 (Biomarkers)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (podocalyxin)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Albuminuria
MH  - Biomarkers/urine
MH  - Creatinine/urine
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus, Type 2/*complications/urine
MH  - Diabetic Nephropathies/*diagnosis/urine
MH  - Early Diagnosis
MH  - Humans
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Sialoglycoproteins/*urine
OTO - NOTNLM
OT  - ROC analysis
OT  - diabetic nephropathy
OT  - microalbumin
OT  - podocalyxin
OT  - podocytes
EDAT- 2020/08/12 06:00
MHDA- 2021/11/09 06:00
CRDT- 2020/08/12 06:00
PHST- 2020/05/25 00:00 [received]
PHST- 2020/08/12 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2020/08/12 06:00 [entrez]
AID - /j/rjim.ahead-of-print/rjim-2020-0023/rjim-2020-0023.xml [pii]
AID - 10.2478/rjim-2020-0023 [doi]
PST - epublish
SO  - Rom J Intern Med. 2020 Dec 17;58(4):233-241. doi: 10.2478/rjim-2020-0023. Print 
      2020 Dec 1.