PMID- 32781023
OWN - NLM
STAT- MEDLINE
DCOM- 20210713
LR  - 20210713
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 53
DP  - 2020 Oct
TI  - Isolation and phylogenetic analysis of Getah virus from a commercial modified 
      live vaccine against porcine reproductive and respiratory syndrome virus.
PG  - 101650
LID - S0890-8508(20)30400-X [pii]
LID - 10.1016/j.mcp.2020.101650 [doi]
AB  - In the present study, Getah virus (GETV) isolate, GETV-V1, was isolated from a 
      commercial PRRSV attenuated live vaccine (MLV), which has been widely used to 
      immunize pigs against porcine reproductive and respiratory syndrome virus 
      (PRRSV). Further analysis demonstrated that nine batches of the PRRSV MLV vaccine 
      (three batches per year from 2017 to 2019) from the same manufacturer were all 
      positive for GETV. Genomic analyses indicated that the GETV-V1 isolate shared the 
      highest sequence identity with the GETV strain, 16-I-674, which was isolated from 
      horses in Japan. The phylogenetic analysis based on the genomic sequences showed 
      that the GETV-V1 strain was clustered with the Japanese GETV strains. Taken 
      together, this is the first report of GETV contamination in live swine vaccines 
      in China. Our findings demonstrate that immunization with commercial live 
      vaccines might be a potential novel route of GETV transmission in swine. This 
      highlights the need for more extensive monitoring of commercial live vaccines.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhou, Feng
AU  - Zhou F
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
FAU - Wang, Aojie
AU  - Wang A
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
FAU - Chen, Lu
AU  - Chen L
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
FAU - Wang, Xingang
AU  - Wang X
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
FAU - Cui, Dandan
AU  - Cui D
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
FAU - Chang, Hongtao
AU  - Chang H
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China. Electronic address: 
      ndcht@163.com.
FAU - Wang, Chuanqing
AU  - Wang C
AD  - College of Animal Veterinary Medicine, Henan Agricultural University, 
      Agricultural Road No. 63, Zhengzhou, Henan, 450002, China. Electronic address: 
      wchuanq@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200808
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Alphavirus/*classification/genetics/isolation & purification
MH  - Animals
MH  - Cell Line
MH  - China
MH  - Drug Contamination
MH  - Horses
MH  - Japan
MH  - Phylogeny
MH  - Phylogeography
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Swine
MH  - Viral Vaccines/*analysis
OTO - NOTNLM
OT  - Getah virus
OT  - Isolation
OT  - Sequence analysis
OT  - Vaccine
EDAT- 2020/08/12 06:00
MHDA- 2021/07/14 06:00
CRDT- 2020/08/12 06:00
PHST- 2020/06/28 00:00 [received]
PHST- 2020/07/23 00:00 [revised]
PHST- 2020/08/07 00:00 [accepted]
PHST- 2020/08/12 06:00 [pubmed]
PHST- 2021/07/14 06:00 [medline]
PHST- 2020/08/12 06:00 [entrez]
AID - S0890-8508(20)30400-X [pii]
AID - 10.1016/j.mcp.2020.101650 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2020 Oct;53:101650. doi: 10.1016/j.mcp.2020.101650. Epub 2020 
      Aug 8.