PMID- 32782088
OWN - NLM
STAT- MEDLINE
DCOM- 20210826
LR  - 20210826
IS  - 1879-291X (Electronic)
IS  - 0301-5629 (Linking)
VI  - 46
IP  - 11
DP  - 2020 Nov
TI  - Characteristic Blood-Perfusion Reduction of Walker 256 Tumor Induced by 
      Diagnostic Ultrasound and Microbubbles.
PG  - 3069-3079
LID - S0301-5629(20)30305-7 [pii]
LID - 10.1016/j.ultrasmedbio.2020.07.009 [doi]
AB  - Tumor angiogenesis is characterized by a defective, leaky and fragile 
      microvascular construction, and microbubble-enhanced ultrasound (MEUS) with 
      high-pressure amplitude is capable of disrupting tumor microvasculature and 
      arresting blood perfusion. In this study, we tried to investigate whether the 
      blood perfusion of a malignant tumor can be characteristically interrupted by 
      combining microbubbles and diagnostic ultrasound (US). Twenty-nine Sprague-Dawley 
      (SD) rats with subcutaneous Walker 256 tumors and seven healthy SD rats were 
      included. Fifteen tumors were treated by MEUS, which combined constant 
      microbubble injection and 20 episodes of irradiation by diagnostic US (i.e., 
      acoustic radiation force impulse [ARFI] imaging). The other 14 tumors were 
      treated by ARFI or sham US only. Seven skeleton muscles from healthy SD rats were 
      also treated with MEUS, serving as the control. Contrast-enhanced ultrasound 
      (CEUS) was performed before and after all treatments. The blood perfusion of the 
      tumor MEUS group showed a significant drop immediately after treatment, followed 
      by a quick, incomplete perfusion recovery within 10-20 min. The visual perfusion 
      scoring result was consistent with the quantitative analysis by CEUS peak 
      intensity. However, there were no significant perfusion changes in the tumor 
      control groups or the muscle control group. Histologic examination found severe 
      microvascular disruption and hemorrhage in the MEUS-treated tumors but not in the 
      control groups. Therefore, the treatment combining diagnostic US and microbubbles 
      can specifically decrease or interrupt the blood perfusion of Walker 256 tumors, 
      which could be a potential new imaging method for diagnosing malignant tumors.
CI  - Copyright (c) 2020 World Federation for Ultrasound in Medicine & Biology. Published 
      by Elsevier Inc. All rights reserved.
FAU - Li, Ningshan
AU  - Li N
AD  - Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, 
      China.
FAU - Liao, Yiyi
AU  - Liao Y
AD  - Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, 
      China.
FAU - Tang, Jiawei
AU  - Tang J
AD  - Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, 
      China. Electronic address: jwtangmd@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200808
PL  - England
TA  - Ultrasound Med Biol
JT  - Ultrasound in medicine & biology
JID - 0410553
SB  - IM
MH  - Animals
MH  - Carcinoma 256, Walker/*blood supply
MH  - Male
MH  - *Microbubbles
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regional Blood Flow
MH  - Ultrasonography
OTO - NOTNLM
OT  - Blood perfusion
OT  - Diagnostic ultrasound
OT  - Microbubble
OT  - Tumor differential diagnosis
COIS- Conflict of interest disclosure The authors declare that they have no conflict of 
      interest to this work.
EDAT- 2020/08/13 06:00
MHDA- 2021/08/27 06:00
CRDT- 2020/08/13 06:00
PHST- 2020/01/14 00:00 [received]
PHST- 2020/07/03 00:00 [revised]
PHST- 2020/07/13 00:00 [accepted]
PHST- 2020/08/13 06:00 [pubmed]
PHST- 2021/08/27 06:00 [medline]
PHST- 2020/08/13 06:00 [entrez]
AID - S0301-5629(20)30305-7 [pii]
AID - 10.1016/j.ultrasmedbio.2020.07.009 [doi]
PST - ppublish
SO  - Ultrasound Med Biol. 2020 Nov;46(11):3069-3079. doi: 
      10.1016/j.ultrasmedbio.2020.07.009. Epub 2020 Aug 8.