PMID- 32782548 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200928 IS - 1792-1074 (Print) IS - 1792-1082 (Electronic) IS - 1792-1074 (Linking) VI - 20 IP - 3 DP - 2020 Sep TI - Small RNA profiles of HTLV-1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T-cell antigen receptor gamma-chain using massively parallel sequencing: A pilot study. PG - 2311-2321 LID - 10.3892/ol.2020.11803 [doi] AB - In the present pilot study, massively parallel sequencing (MPS) technology was used to investigate cellular small RNA (sRNA) levels in the peripheral blood mononuclear cells (PBMCs) of human T-lymphotropic virus type I (HTLV-I) infected asymptomatic carriers with monoclonal (ASM) and polyclonal (ASP) T cell receptor (TCR) gamma gene. Blood samples from 15 HTLV-I asymptomatic carriers (seven ASM and eight ASP) were tested for the clonal TCR-gamma gene and submitted for sRNA library construction together with blood samples of five healthy controls (HCs) using Illumina sequencing platform. The sRNA-sequencing reads were aligned, annotated and profiled using various bioinformatics tools. Based on these results, possible markers were validated in the study samples by performing reverse transcription-quantitative (RT-q)PCR analysis. A total of 76 known sRNAs and 52 putative novel sRNAs were identified. Among them, 44 known and 34 potential novel sRNAs were differentially expressed in the ASM and ASP libraries compared with HCs. In addition, 10 known sRNAs were exclusively dysregulated in the ASM group and one (transfer RNA 65) was significantly upregulated in the ASP group. Homo sapiens (hsa) microRNA (miRNA/mir)-23a-3p, -28-5p, hsa-let-7e-5p and hsa-mir-28-3p and -361-5p were the most abundantly upregulated mature miRNAs and hsa-mir-363-3p, -532-5p, -106a-5p, -25-3p and -30e-5p were significantly downregulated miRNAs (P<0.05) with a >2-fold difference between the ASM and ASP groups compared with HCs. Based on these results, hsa-mir-23a-3p and -363-3p were selected for additional validation. However, the quantification of these two miRNAs using RT-qPCR did not provide any significant differences. While the present study failed to identify predictive sRNA markers to distinguish between ASM and ASP, the MPS results revealed differential sRNA expression profiles in the PBMCs of HTLV-1 asymptomatic carriers (ASM and ASP) compared with HCs. CI - Copyright: (c) Valadao de Souza et al. FAU - Valadao de Souza, Daniela Raguer AU - Valadao de Souza DR AD - Laboratory of Dermatology and Immunodeficiency, Department of Dermatology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Pessoa, Rodrigo AU - Pessoa R AD - Laboratory of Dermatology and Immunodeficiency, Department of Dermatology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Nascimento, Andrezza AU - Nascimento A AD - Laboratory of Dermatology and Immunodeficiency, Department of Dermatology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Nukui, Youko AU - Nukui Y AD - Department of Hematology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Pereira, Juliana AU - Pereira J AD - Department of Hematology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Casseb, Jorge AU - Casseb J AD - Laboratory of Dermatology and Immunodeficiency, Department of Dermatology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Penalva de Oliveira, Augusto Cesar AU - Penalva de Oliveira AC AD - Department of Neurology, Emilio Ribas Institute of Infectious Diseases, Sao Paulo 01246-900, Brazil. FAU - da Silva Duarte, Alberto Jose AU - da Silva Duarte AJ AD - Laboratory of Dermatology and Immunodeficiency, Department of Dermatology, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. FAU - Clissa, Patricia Bianca AU - Clissa PB AD - Immunopathology Laboratory, Butantan Institute, Sao Paulo 05503-900, Brazil. FAU - Sanabani, Sabri Saeed AU - Sanabani SS AD - Laboratory of Medical Investigation Unit 03,Clinics Hospital, Faculty of Medicine, University of Sao Paulo, Sao Paulo 05403 000, Brazil. LA - eng PT - Journal Article DEP - 20200701 PL - Greece TA - Oncol Lett JT - Oncology letters JID - 101531236 PMC - PMC7400997 OTO - NOTNLM OT - T-cell antigen receptor OT - asymptomatic carriers OT - human T-lymphotropic virus type I OT - massively parallel sequencing OT - small RNA EDAT- 2020/08/13 06:00 MHDA- 2020/08/13 06:01 PMCR- 2020/07/01 CRDT- 2020/08/13 06:00 PHST- 2019/12/19 00:00 [received] PHST- 2020/05/21 00:00 [accepted] PHST- 2020/08/13 06:00 [entrez] PHST- 2020/08/13 06:00 [pubmed] PHST- 2020/08/13 06:01 [medline] PHST- 2020/07/01 00:00 [pmc-release] AID - OL-0-0-11803 [pii] AID - 10.3892/ol.2020.11803 [doi] PST - ppublish SO - Oncol Lett. 2020 Sep;20(3):2311-2321. doi: 10.3892/ol.2020.11803. Epub 2020 Jul 1.