PMID- 32783540
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 1552-695X (Electronic)
IS  - 1534-7354 (Print)
IS  - 1534-7354 (Linking)
VI  - 19
DP  - 2020 Jan-Dec
TI  - Study of the Radiosensitizing and Radioprotective Efficacy of Bromelain (a 
      Pineapple Extract): In Vitro and In Vivo.
PG  - 1534735420950468
LID - 10.1177/1534735420950468 [doi]
LID - 1534735420950468
AB  - This study hypothesizes that, bromelain (BL) acts as radiosensitizer of tumor 
      cells and that it protects normal cells from radiation effects. In vitro and in 
      vivo studies have been carried out to prove that assumption. In vitro MTT cell 
      proliferation assay has shown that the irradiated Ehrlich ascites carcinoma (EAC) 
      cell line could be sensitized by BL pretreatment. In vivo: animals were randomly 
      divided into 5 groups, Group 1: control (PBS i.p for 10 days), Group 2: Ehrlich 
      solid tumor (EST) bearing mice, Group 3: EST + gamma-radiation (fractionated dose, 
      1 Gy x 5), Group 4: EST + BL (6 mg/kg, i.p), daily for 10 days, Group 5: EST + BL 
      for 10 days followed by gamma-irradiation (1 Gy x 5). The size and weight of tumors 
      in gamma-irradiated EST bearing mice treated with BL decreased significantly with 
      a significant amelioration in the histopathological examination. Besides, BL 
      mitigated the effect of gamma-irradiation on the liver relative gene expression of 
      poly ADP ribose polymerase-1 (PARP1), nuclear factor kappa activated B cells 
      (NF-kappaB), and peroxisome proliferator-activated receptor alpha (PPAR-alpha), and it 
      restored liver function via amelioration of paraoxonase1 (PON1) activity, 
      reactive oxygen species (ROS) content, lipid peroxidation (LPO) and serum 
      aspartate transaminase (AST), alanine transaminase (ALT), and albumin (ALB). It 
      is concluded that BL can be considered as a radio-sensitizer and radio-protector, 
      suggesting a possible role in reducing radiation exposure dose during 
      radiotherapy.
FAU - Mekkawy, Mai H
AU  - Mekkawy MH
AD  - Drug Radiation Research Department, National Centre for Radiation Research and 
      Technology, Egyptian, Atomic Energy Authority, Nasr City, Cairo, Egypt.
FAU - Fahmy, Hanan A
AU  - Fahmy HA
AUID- ORCID: 0000-0002-9593-9664
AD  - Drug Radiation Research Department, National Centre for Radiation Research and 
      Technology, Egyptian, Atomic Energy Authority, Nasr City, Cairo, Egypt.
FAU - Nada, Ahmed S
AU  - Nada AS
AD  - Drug Radiation Research Department, National Centre for Radiation Research and 
      Technology, Egyptian, Atomic Energy Authority, Nasr City, Cairo, Egypt.
FAU - Ali, Ola S
AU  - Ali OS
AD  - Biochemistry Department, Al-Azhar University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Integr Cancer Ther
JT  - Integrative cancer therapies
JID - 101128834
RN  - 0 (Plant Extracts)
RN  - 9001-00-7 (Bromelains)
SB  - IM
MH  - *Ananas
MH  - Animals
MH  - Bromelains/pharmacology
MH  - *Carcinoma, Ehrlich Tumor/drug therapy/radiotherapy
MH  - Gamma Rays
MH  - Lipid Peroxidation
MH  - Mice
MH  - Plant Extracts
PMC - PMC7425266
OTO - NOTNLM
OT  - bromelain
OT  - radio-protector
OT  - radio-sensitizer
OT  - tumor
OT  - gamma-radiation
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2020/08/14 06:00
MHDA- 2021/08/19 06:00
PMCR- 2020/08/12
CRDT- 2020/08/14 06:00
PHST- 2020/08/14 06:00 [entrez]
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
PHST- 2020/08/12 00:00 [pmc-release]
AID - 10.1177_1534735420950468 [pii]
AID - 10.1177/1534735420950468 [doi]
PST - ppublish
SO  - Integr Cancer Ther. 2020 Jan-Dec;19:1534735420950468. doi: 
      10.1177/1534735420950468.