PMID- 32784005
OWN - NLM
STAT- MEDLINE
DCOM- 20211029
LR  - 20211029
IS  - 1873-2747 (Electronic)
IS  - 0361-9230 (Linking)
VI  - 164
DP  - 2020 Nov
TI  - Maternal separation plus social isolation during adolescence reprogram brain 
      dopamine and endocannabinoid systems and facilitate alcohol intake in rats.
PG  - 21-28
LID - S0361-9230(20)30583-9 [pii]
LID - 10.1016/j.brainresbull.2020.08.002 [doi]
AB  - Adverse early life experiences, i.e. abusive parenting, during postnatal 
      development, induce long-lasting effects on the stress response systems and 
      behavior. Such changes persist throughout an individual's life, making him/her 
      vulnerable to suffer psychiatric disorders, including anxiety disorders and drug 
      addiction. Rat pup maternal separation (MS) is a widely used rodent early-life 
      stress model. MS induces changes in the dopamine and endocannabinoid systems in 
      the nucleus accumbens (NAcc) that facilitate alcohol consumption. In this study, 
      our endeavor was to determine if social isolation during adolescence (aSI) was as 
      efficient as MS to facilitate alcohol intake; and moreover, if their combination 
      (MS + aSI) induces even higher alcohol intake and exacerbates anxiety-like 
      behaviors. Also, we evaluated dopamine and endocannabinoid receptors in the NAcc 
      to describe potential changes caused by MS, aSI or both. Wistar rats were reared 
      under 4 different conditions: non-MS + social housing (SH), MS + SH, non-MS + aSI 
      and MS + aSI. Once these rats became adults they were submitted to a voluntary 
      alcohol intake protocol for 10 days. Similar groups of rats with no exposure to 
      alcohol whatsoever, were sacrificed to dissect out the NAcc to analyze the 
      expression of cannabinoid (CB1R and CB2R) and dopamine (D2R and D3R) receptors. 
      Results showed that MS, aSI and MS + aSI increase both CB1R, D2R and D3R 
      expression in the NAcc and also increase alcohol intake and anxiety. These 
      results suggest that early life adverse experiences induce a reprogramming of the 
      brain's dopamine and endocannabinoid systems which increases subject's 
      vulnerability to develop anxiety, alcohol abuse and dependence.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Amancio-Belmont, Octavio
AU  - Amancio-Belmont O
AD  - Laboratorio de Canabinoides, Departamento de Fisiologia, Facultad de Medicina, 
      Universidad Nacional Autonoma de Mexico, Mexico.
FAU - Becerril Melendez, Alline L
AU  - Becerril Melendez AL
AD  - Laboratorio de Canabinoides, Departamento de Fisiologia, Facultad de Medicina, 
      Universidad Nacional Autonoma de Mexico, Mexico.
FAU - Ruiz-Contreras, Alejandra E
AU  - Ruiz-Contreras AE
AD  - Laboratorio de Neurogenomica Cognitiva, Departamento de Psicofisiologia, Facultad 
      de Psicologia. Universidad Nacional Autonoma de Mexico, Mexico.
FAU - Mendez-Diaz, Monica
AU  - Mendez-Diaz M
AD  - Laboratorio de Canabinoides, Departamento de Fisiologia, Facultad de Medicina, 
      Universidad Nacional Autonoma de Mexico, Mexico.
FAU - Prospero-Garcia, Oscar
AU  - Prospero-Garcia O
AD  - Laboratorio de Canabinoides, Departamento de Fisiologia, Facultad de Medicina, 
      Universidad Nacional Autonoma de Mexico, Mexico. Electronic address: opg@unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200809
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Endocannabinoids)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Alcohol Drinking/*metabolism
MH  - Animals
MH  - Brain/*metabolism
MH  - Dopamine/*metabolism
MH  - Endocannabinoids/*metabolism
MH  - *Maternal Deprivation
MH  - Rats
MH  - Rats, Wistar
MH  - *Social Isolation
MH  - Stress, Psychological/metabolism
OTO - NOTNLM
OT  - Alcohol intake
OT  - Anxiety
OT  - Dopaminergic system
OT  - Endocannabinoid system
OT  - Maternal separation
OT  - Social isolation
EDAT- 2020/08/14 06:00
MHDA- 2021/10/30 06:00
CRDT- 2020/08/14 06:00
PHST- 2020/04/26 00:00 [received]
PHST- 2020/07/18 00:00 [revised]
PHST- 2020/08/02 00:00 [accepted]
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2021/10/30 06:00 [medline]
PHST- 2020/08/14 06:00 [entrez]
AID - S0361-9230(20)30583-9 [pii]
AID - 10.1016/j.brainresbull.2020.08.002 [doi]
PST - ppublish
SO  - Brain Res Bull. 2020 Nov;164:21-28. doi: 10.1016/j.brainresbull.2020.08.002. Epub 
      2020 Aug 9.