PMID- 32784741
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210308
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 16
DP  - 2020 Aug 7
TI  - Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of 
      Parkinson's Disease.
LID - 10.3390/molecules25163602 [doi]
LID - 3602
AB  - Parkinson's disease (PD) is a well-known age-related neurodegenerative disease. 
      Considering the vital importance of disease modeling based on reprogramming 
      technology, we adopted direct reprogramming to human-induced neuronal progenitor 
      cells (hiNPCs) for in vitro assessment of potential therapeutics. In this study, 
      we investigated the neuroprotective effects of cryptotanshinone (CTN), which has 
      been reported to have antioxidant properties, through PD patient-derived hiNPCs 
      (PD-iNPCs) model with induced oxidative stress and cell death by the proteasome 
      inhibitor MG132. A cytotoxicity assay showed that CTN possesses anti-apoptotic 
      properties in PD-hiNPCs. CTN treatment significantly reduced cellular apoptosis 
      through mitochondrial restoration, such as the reduction in mitochondrial 
      reactive oxygen species and increments of mitochondrial membrane potential. These 
      effects of CTN are mediated via the nuclear factor erythroid 2-related factor 2 
      (NRF2) pathway in PD-hiNPCs. Consequently, CTN could be a potential antioxidant 
      reagent for preventing disease-related pathological phenotypes of PD.
FAU - Lee, Joo-Eun
AU  - Lee JE
AUID- ORCID: 0000-0002-2495-1439
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
FAU - Sim, Hyuna
AU  - Sim H
AUID- ORCID: 0000-0002-3051-8076
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
AD  - Department of Functional Genomics, KRIBB School of Bioscience, University of 
      Science and Technology, Daejeon 34113, Korea.
FAU - Yoo, Hee Min
AU  - Yoo HM
AUID- ORCID: 0000-0002-5951-2137
AD  - Group for Biometrology, Korea Research Institute of Standards and Science 
      (KRISS), Daejeon 34113, Korea.
FAU - Lee, Minhyung
AU  - Lee M
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
FAU - Baek, Aruem
AU  - Baek A
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
FAU - Jeon, Young-Joo
AU  - Jeon YJ
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
FAU - Seo, Kang-Sik
AU  - Seo KS
AD  - Huen Co., Ltd., Gwanggyo Business Center 5F (#508), 156, Gwanggyo-ro, 
      Yeongtong-gu, Suwon 16506, Korea.
FAU - Son, Mi-Young
AU  - Son MY
AUID- ORCID: 0000-0001-7590-8812
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
AD  - Department of Functional Genomics, KRIBB School of Bioscience, University of 
      Science and Technology, Daejeon 34113, Korea.
FAU - Yoon, Joo Seog
AU  - Yoon JS
AD  - Huen Co., Ltd., Gwanggyo Business Center 5F (#508), 156, Gwanggyo-ro, 
      Yeongtong-gu, Suwon 16506, Korea.
FAU - Kim, Janghwan
AU  - Kim J
AUID- ORCID: 0000-0003-3227-958X
AD  - Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Daejeon 34141, Korea.
AD  - Department of Functional Genomics, KRIBB School of Bioscience, University of 
      Science and Technology, Daejeon 34113, Korea.
LA  - eng
GR  - 2015M3A9C7030128/National Research Foundation of Korea/
GR  - NRF-2018M3A9H3023077/National Research Foundation of Korea/
GR  - KGS1092012/Korea Research Institute of Bioscience and Biotechnology/
PT  - Journal Article
DEP - 20200807
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Leupeptins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Phenanthrenes)
RN  - 5E9SXT166N (cryptotanshinone)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - Case-Control Studies
MH  - Cell Line
MH  - Cellular Reprogramming/*drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Induced Pluripotent Stem Cells/cytology/drug effects/metabolism
MH  - Leupeptins/pharmacology
MH  - Mitochondria/metabolism
MH  - Neuroprotective Agents/*pharmacology/therapeutic use
MH  - Oxidative Stress/drug effects
MH  - Parkinson Disease/*drug therapy/metabolism/pathology
MH  - Phenanthrenes/*pharmacology
PMC - PMC7463464
OTO - NOTNLM
OT  - Parkinson's disease
OT  - antioxidant
OT  - cryptotanshinone
OT  - disease modeling
OT  - mitochondrial dysfunction
COIS- The authors declare no conflict of interest.
EDAT- 2020/08/14 06:00
MHDA- 2021/03/09 06:00
PMCR- 2020/08/07
CRDT- 2020/08/14 06:00
PHST- 2020/07/21 00:00 [received]
PHST- 2020/08/02 00:00 [revised]
PHST- 2020/08/05 00:00 [accepted]
PHST- 2020/08/14 06:00 [entrez]
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/08/07 00:00 [pmc-release]
AID - molecules25163602 [pii]
AID - molecules-25-03602 [pii]
AID - 10.3390/molecules25163602 [doi]
PST - epublish
SO  - Molecules. 2020 Aug 7;25(16):3602. doi: 10.3390/molecules25163602.