PMID- 32785473
OWN - NLM
STAT- MEDLINE
DCOM- 20200928
LR  - 20200928
IS  - 1807-3107 (Electronic)
IS  - 1806-8324 (Linking)
VI  - 34
DP  - 2020
TI  - Qualitative and quantitative molecular analysis of bacteria in root canals of 
      primary teeth with pulp necrosis.
PG  - e093
LID - S1806-83242020000100265 [pii]
LID - 10.1590/1807-3107bor-2020.vol34.0093 [doi]
AB  - Information about bacterial diversity, such as the number of each species in the 
      root canals of primary teeth, contributes to improving our effective management 
      of infections of endodontic origin in primary teeth. This study made a 
      qualitative and quantitative assessment of the bacteria in the root canals of 
      primary teeth with necrotic pulp, using the fluorescence in situ hybridization 
      (FISH) technique. Thirty-one primary teeth with pulp necrosis from 31 children 
      were evaluated using the FISH technique, to detect the presence and density of 
      Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Enterococcus 
      faecalis, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella 
      intermedia, Prevotella nigrescens, Streptococcus, Streptococcus mutans, 
      Streptococcus sobrinus, Tannerella forsythia and Treponema denticola. Descriptive 
      measures explained the data related to density, and Student's t-test assessed the 
      differences among the densities of each bacterium, according to signs and 
      symptoms. The bacterial density was paired and correlated. All bacteria tested 
      were detected and identified in all the samples. The average number of bacterial 
      individuals from each species ranged from 1.9 x 108 cells/mL (S. mutans) to 3.1 x 
      108 cells/mL (F. nucleatum) (p > 0.05). The sum of the mean counts of each 
      bacterium represented almost 80% of the entire microbial community. Patients with 
      pain had significantly more T. denticola, and those with edema showed a greater 
      density of Streptococcus and P. nigrescens (p < 0.05). This study revealed that 
      all 12 bacteria evaluated were found in all primary teeth with pulp necrosis. 
      There was no predominance among the species studied; all species had a similar 
      number of individuals.
FAU - Lemos, Samira Salomao
AU  - Lemos SS
AD  - Postgraduate Program in Dentistry, Universidade Federal de Juiz de Fora, Juiz de 
      Fora, MG, Brazil.
FAU - Cesar, Dioneia Evangelista
AU  - Cesar DE
AD  - Department of Ecology and Molecular Biology of Microorganisms, Biological 
      Sciences Institute, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, 
      Brazil.
FAU - ProcOpio, Stefania Werneck
AU  - ProcOpio SW
AD  - Postgraduate Program in Dentistry, School of Dentistry, Universidade Federal de 
      Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Machado, Fernanda Campos
AU  - Machado FC
AD  - Department of Social and Child Dentistry, School of Dentistry, Universidade 
      Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
FAU - Ribeiro, Luiz Claudio
AU  - Ribeiro LC
AD  - Department of Statistics, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, 
      Brazil.
FAU - Ribeiro, Rosangela Almeida
AU  - Ribeiro RA
AD  - Department of Social and Child Dentistry, School of Dentistry, Universidade 
      Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20200807
PL  - Brazil
TA  - Braz Oral Res
JT  - Brazilian oral research
JID - 101307187
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Child
MH  - DNA, Bacterial
MH  - *Dental Pulp Cavity
MH  - *Dental Pulp Necrosis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Porphyromonas gingivalis
MH  - Prevotella intermedia
MH  - *Tooth, Deciduous
EDAT- 2020/08/14 06:00
MHDA- 2020/09/29 06:00
CRDT- 2020/08/14 06:00
PHST- 2020/03/09 00:00 [received]
PHST- 2020/05/18 00:00 [accepted]
PHST- 2020/08/14 06:00 [entrez]
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2020/09/29 06:00 [medline]
AID - S1806-83242020000100265 [pii]
AID - 10.1590/1807-3107bor-2020.vol34.0093 [doi]
PST - ppublish
SO  - Braz Oral Res. 2020;34:e093. doi: 10.1590/1807-3107bor-2020.vol34.0093. Epub 2020 
      Aug 7.