PMID- 32786312
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20220531
IS  - 1948-7193 (Electronic)
IS  - 1948-7193 (Linking)
VI  - 11
IP  - 17
DP  - 2020 Sep 2
TI  - MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances 
      Their Efficacy in Neuroinflammation Injured Brain through Inhibiting 
      NLRP3/Caspase-1/IL-1beta Axis.
PG  - 2590-2601
LID - 10.1021/acschemneuro.0c00268 [doi]
AB  - The inflammatory microenvironment in a lesion is not conducive to the survival of 
      stem cells. Improving the inflammatory microenvironment may be an alternative 
      strategy to enhance the efficacy of stem cells. We evaluated the therapeutic 
      effect and molecular mechanism of mitsugumin53 (MG53) on lipopolysaccharide 
      (LPS)-induced damage in human umbilical cord mesenchymal stem cells (hUC-MSCs) 
      and in C57/BL6 mice. MG53 significantly promoted the proliferation and migration 
      of hUC-MSCs, protected hUC-MSCs against LPS-induced apoptosis and mitochondrial 
      dysfunction, and reversed LPS-induced inflammatory cytokine release. Furthermore, 
      MG53 combined with hUC-MSCs transplantation improved LPS-induced memory 
      impairment and activated neurogenesis by promoting the migration of hUC-MSCs and 
      enhancing betaIII-tubulin and doublecortin (DCX) expression. MG53 protein combined 
      with hUC-MSCs improved the M1/M2 phenotype polarization of microglia accompanied 
      by lower inducible nitric oxide synthase (iNOS) expression and higher arginase 1 
      (ARG1) expression. MG53 significantly suppressed the expression of tumor necrosis 
      factor alpha (TNF-alpha), Toll-like receptor 4 (TLR4), nucleotide oligomerization 
      domain-like receptor protein 3 (NLRP3), cleaved-caspase-1, and interleukin 
      (IL)-1beta to alleviate LPS-induced neuroinflammation on hUC-MSCs and C57/BL6 mice. 
      In conclusion, our results indicated that MG53 could protect hUC-MSCs against 
      LPS-induced inflammatory damage and facilitate their efficacy in LPS-treated 
      C57/BL6 mice partly by inhibiting the NLRP3/caspase-1/IL-1beta axis.
FAU - Ma, Shanshan
AU  - Ma S
AUID- ORCID: 0000-0002-0780-4568
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
AD  - Institute of Neuroscience, Zhengzhou University, Zhengzhou, 450052 Henan, China.
FAU - Wang, Yaping
AU  - Wang Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Zhou, Xinkui
AU  - Zhou X
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Li, Zhe
AU  - Li Z
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Zhang, Zhenkun
AU  - Zhang Z
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Wang, Yingying
AU  - Wang Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Huang, Tuanjie
AU  - Huang T
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Zhang, Yanting
AU  - Zhang Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
FAU - Shi, Jijing
AU  - Shi J
AD  - Central Lab of the First People's Hospital of Zhengzhou, Zhengzhou, 450001 Henan. 
      China.
FAU - Guan, Fangxia
AU  - Guan F
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
AD  - Institute of Neuroscience, Zhengzhou University, Zhengzhou, 450052 Henan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200817
PL  - United States
TA  - ACS Chem Neurosci
JT  - ACS chemical neuroscience
JID - 101525337
RN  - 0 (Dcx protein, mouse)
RN  - 0 (Doublecortin Protein)
RN  - 0 (MG53 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Brain
MH  - Caspase 1
MH  - Doublecortin Protein
MH  - Membrane Proteins
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mice
MH  - *NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Umbilical Cord
OTO - NOTNLM
OT  - LPS
OT  - MG53
OT  - NLRP3 inflammasome
OT  - hUC-MSCs
OT  - neuroinflammation
EDAT- 2020/08/14 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/08/14 06:00
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/08/14 06:00 [entrez]
AID - 10.1021/acschemneuro.0c00268 [doi]
PST - ppublish
SO  - ACS Chem Neurosci. 2020 Sep 2;11(17):2590-2601. doi: 
      10.1021/acschemneuro.0c00268. Epub 2020 Aug 17.