PMID- 32786430
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20210216
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 92
IP  - 18
DP  - 2020 Sep 15
TI  - Direct Quantification of Single Red Blood Cell Hemoglobin Concentration with 
      Multiphoton Microscopy.
PG  - 12235-12241
LID - 10.1021/acs.analchem.0c01609 [doi]
AB  - Blood disorders, diseases, and infections often affect the shape, number, and 
      content of red blood cells (RBCs) dramatically. To combat these pathologies, many 
      therapies target RBCs and their contents directly. Mean corpuscular hemoglobin 
      concentration (MCHC) is an important pathological metric in both identification 
      and treatment. However, current methods for RBC analysis and MCHC quantification 
      rely on bulk measurements. Single RBC measurements could provide necessary 
      insight into the heterogeneity of RBC health and improve therapeutic efficacy. In 
      this study, we present a novel multimodal multiphoton approach for quantifying 
      hemoglobin concentration at single RBC resolution. We achieve this by collecting 
      two images simultaneously that allows us to excite water with stimulated Raman 
      scattering and hemoglobin with transient absorption. This multimodal imaging is 
      enabled by a newly designed orthogonal modulation theme for dual-channel lock-in 
      detection. By leveraging water as an internal standard, we quantify MCHC of 
      healthy RBCs and RBCs infected with Plasmodium yoelii, a commonly studied rodent 
      parasite model.
FAU - Francis, Andrew T
AU  - Francis AT
AUID- ORCID: 0000-0003-3192-5260
AD  - Department of Chemistry, University of Washington, Seattle, Washington 98195, 
      United States.
FAU - Shears, Melanie J
AU  - Shears MJ
AUID- ORCID: 0000-0002-8226-4354
AD  - Department of Laboratory Medicine and Center for Emerging and Re-emerging 
      Infectious Diseases, University of Washington, 750 Republican Street, Seattle, 
      Washington 98109, United States.
FAU - Murphy, Sean C
AU  - Murphy SC
AD  - Department of Laboratory Medicine and Center for Emerging and Re-emerging 
      Infectious Diseases, University of Washington, 750 Republican Street, Seattle, 
      Washington 98109, United States.
FAU - Fu, Dan
AU  - Fu D
AUID- ORCID: 0000-0001-9243-8306
AD  - Department of Chemistry, University of Washington, Seattle, Washington 98195, 
      United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200825
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Hemoglobins)
SB  - IM
MH  - Animals
MH  - Erythrocytes/*chemistry/parasitology
MH  - Hemoglobins/*analysis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Microscopy, Fluorescence, Multiphoton
MH  - *Single-Cell Analysis
EDAT- 2020/08/14 06:00
MHDA- 2021/02/17 06:00
CRDT- 2020/08/14 06:00
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/08/14 06:00 [entrez]
AID - 10.1021/acs.analchem.0c01609 [doi]
PST - ppublish
SO  - Anal Chem. 2020 Sep 15;92(18):12235-12241. doi: 10.1021/acs.analchem.0c01609. 
      Epub 2020 Aug 25.