PMID- 32789524
OWN - NLM
STAT- MEDLINE
DCOM- 20201223
LR  - 20210209
IS  - 1938-291X (Electronic)
IS  - 0022-0493 (Linking)
VI  - 113
IP  - 4
DP  - 2020 Aug 13
TI  - Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, 
      Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits 
      in Seedling, Single Plant, and Diet-Toxicity Assays.
PG  - 1955-1962
LID - 10.1093/jee/toaa107 [doi]
AB  - The northern corn rootworm (NCR), Diabrotica barberi Smith & Lawrence, is an 
      economic pest of maize in the U.S. Corn Belt. The objective of this study was to 
      determine the baseline susceptibility of a laboratory NCR strain to Bt proteins 
      eCry3.1Ab, mCry3A, Cry3Bb1, and Cry34/35Ab1 using seedling, single plant, and 
      diet-toxicity assays. Plant assays were performed in greenhouse using corn 
      hybrids expressing one of the Bt proteins and each respective near-isoline. 
      Diet-toxicity assays, consisting of Bt proteins overlaid onto artificial diet 
      were also conducted. In both plant assays, significantly more larvae survived 
      Cry34/35Ab1-expressing corn compared with all other Bt-expressing corn, and 
      larvae that survived eCry3.1Ab-expressing corn had significantly smaller head 
      capsule widths compared with larvae that survived Cry34/35Ab1-expressing corn. In 
      seedling assays, larvae surviving eCry3.1Ab-expressing corn also had 
      significantly smaller head capsule widths compared with larvae that survived 
      mCry3A-expressing corn. Additionally, larvae that survived mCry3A-expressing corn 
      weighed significantly more than larvae surviving eCry3.1Ab- and 
      Cry34/35Ab1-expressing corn. In single plant assays, no significant differences 
      in larval dry weight was observed between any of the Bt-expressing corn. In diet 
      assays, LC50s ranged from 0.14 (eCry3.1Ab) to 10.6 microg/cm2 (Cry34/35Ab1), EC50s 
      ranged from 0.12 (Cry34/35Ab1) to 1.57 microg/cm2 (mCry3A), IC50s ranged from 0.08 
      (eCry3.1Ab) to 2.41 microg/cm2 (Cry34/35Ab1), and MIC50s ranged from 2.52 (eCry3.1Ab) 
      to 14.2 microg/cm2 (mCry3A). These results establish the toxicity of four Bt proteins 
      to a laboratory diapausing NCR strain established prior to the introduction of Bt 
      traits and are important for monitoring resistance evolution in NCR field 
      populations.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of 
      Entomological Society of America. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Pereira, Adriano E
AU  - Pereira AE
AD  - Division of Plant Sciences, University of Missouri, Columbia, MO.
FAU - Huynh, Man P
AU  - Huynh MP
AD  - Division of Plant Sciences, University of Missouri, Columbia, MO.
FAU - Sethi, Amit
AU  - Sethi A
AD  - Corteva Agriscience, Johnston, IA.
FAU - Miles, Ashley L
AU  - Miles AL
AD  - Corteva Agriscience, Johnston, IA.
FAU - Wade French, B
AU  - Wade French B
AD  - North Central Agricultural Research Laboratory, Brookings, SD.
FAU - Ellersieck, Mark R
AU  - Ellersieck MR
AD  - Department of Statistics, University of Missouri, Columbia, MO.
FAU - Coudron, Thomas A
AU  - Coudron TA
AD  - Biological Control Insect Research Laboratory, USDA-ARS, Columbia, MO.
FAU - Shelby, Kent S
AU  - Shelby KS
AD  - Biological Control Insect Research Laboratory, USDA-ARS, Columbia, MO.
FAU - Hibbard, Bruce E
AU  - Hibbard BE
AD  - Plant Genetics Research Unit, USDA/ARS, University of Missouri, Columbia, MO.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Econ Entomol
JT  - Journal of economic entomology
JID - 2985127R
RN  - 0 (Bacterial Proteins)
RN  - 0 (Endotoxins)
RN  - 0 (Hemolysin Proteins)
SB  - IM
EIN - J Econ Entomol. 2020 Oct 16;113(5):2568. PMID: 32856052
MH  - Animals
MH  - *Bacillus thuringiensis/genetics
MH  - Bacterial Proteins
MH  - *Coleoptera/genetics
MH  - Diet
MH  - Endotoxins
MH  - Hemolysin Proteins
MH  - Laboratories
MH  - Larva
MH  - Pest Control, Biological
MH  - Plants, Genetically Modified/genetics
MH  - Seedlings
MH  - Zea mays/genetics
OTO - NOTNLM
OT  - Diabrotica
OT  - Bt corn
OT  - baseline susceptibility
OT  - diet-toxicity assay
OT  - resistance management
EDAT- 2020/08/14 06:00
MHDA- 2020/12/29 06:00
CRDT- 2020/08/14 06:00
PHST- 2020/01/31 00:00 [received]
PHST- 2020/08/14 06:00 [entrez]
PHST- 2020/08/14 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
AID - 5858459 [pii]
AID - 10.1093/jee/toaa107 [doi]
PST - ppublish
SO  - J Econ Entomol. 2020 Aug 13;113(4):1955-1962. doi: 10.1093/jee/toaa107.