PMID- 32796585
OWN - NLM
STAT- MEDLINE
DCOM- 20210217
LR  - 20210217
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 16
DP  - 2020 Aug 11
TI  - Comparing Gene Expression in the Parabrachial and Amygdala of Diestrus and 
      Proestrus Female Rats after Orofacial Varicella Zoster Injection.
LID - 10.3390/ijms21165749 [doi]
LID - 5749
AB  - The orofacial pain pathway projects to the parabrachial and amygdala, and sex 
      steroids have been shown to affect neuronal activity in these regions. GABA 
      positive cells in the amygdala are influenced by sex steroid metabolites to 
      affect pain, and sex steroids have been shown to alter the expression of genes in 
      the parabrachial, changing neuronal excitability. Mechanisms by which sex 
      steroids affect amygdala and parabrachial signaling are unclear. The expression 
      of genes in the parabrachial and amygdala in diestrus (low estradiol) and 
      proestrus (high estradiol) female rats were evaluated in this study. First, 
      varicella zoster virus was injected into the whisker pad of female rats to induce 
      a pain response. Second, gene expression was quantitated using RNA-seq one week 
      after injection. Genes that had the greatest change in expression and known to 
      function in pain signaling were selected for the quantitation of protein content. 
      Protein expression of four genes in the parabrachial and seven genes in the 
      amygdala were quantitated by ELISA. In the parabrachial, neurexin 3 (Nrnx3) was 
      elevated at proestrus. Nrnx3 has a role in AMPA receptor and GABA signaling. 
      Neuronatin (Nnat) and protein phosphatase, Mg(2+)/Mn(2+) dependent 1E (Ppm1e) 
      were elevated in the parabrachial of diestrus animals both genes having a role in 
      pain signaling. Epoxide hydroxylase (Ephx2) was elevated in the parabrachial at 
      proestrus and the vitamin D receptor (Vdr) was elevated in the amygdala. Ephx2 
      antagonists and vitamin D have been used to treat neuropathic pain. In 
      conclusion, sex steroids regulate genes in the parabrachial and amygdala that 
      might result in the greater pain response observed during diestrus.
FAU - Hornung, Rebecca
AU  - Hornung R
AUID- ORCID: 0000-0002-7629-8144
AD  - Department of Biological Sciences, Texas A&M University College of Dentistry, 
      Dallas, TX 75246, USA.
FAU - Pritchard, Addison
AU  - Pritchard A
AD  - Department of Biological Sciences, Texas A&M University College of Dentistry, 
      Dallas, TX 75246, USA.
FAU - Kinchington, Paul R
AU  - Kinchington PR
AUID- ORCID: 0000-0002-1901-9970
AD  - Departments of Ophthalmology and Molecular Microbiology and Genetics, University 
      of Pittsburgh, 203 Lothrop Street, Pittsburgh, PA 15213, USA.
FAU - Kramer, Phillip R
AU  - Kramer PR
AUID- ORCID: 0000-0003-0117-542X
AD  - Department of Biological Sciences, Texas A&M University College of Dentistry, 
      Dallas, TX 75246, USA.
LA  - eng
GR  - NS064022/NS/NINDS NIH HHS/United States
GR  - R01 DE026749/DE/NIDCR NIH HHS/United States
GR  - EY08098/EY/NEI NIH HHS/United States
GR  - DE026749/DE/NIDCR NIH HHS/United States
GR  - P30 EY008098/EY/NEI NIH HHS/United States
GR  - R01 NS064022/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20200811
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Calcitriol)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - EC 3.3.2.10 (EPHX2 protein, rat)
SB  - IM
MH  - Amygdala/*metabolism
MH  - Animals
MH  - Diestrus/*genetics
MH  - Epoxide Hydrolases/metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Herpesvirus 3, Human/*physiology
MH  - *Injections
MH  - Nerve Tissue Proteins/metabolism
MH  - Neuralgia/genetics
MH  - Proestrus/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Receptors, Calcitriol/metabolism
PMC - PMC7461146
OTO - NOTNLM
OT  - herpes zoster
OT  - orofacial
OT  - pain
OT  - shingles
OT  - steroids
COIS- The authors declare no conflict of interest.
EDAT- 2020/08/17 06:00
MHDA- 2021/02/18 06:00
PMCR- 2020/08/01
CRDT- 2020/08/16 06:00
PHST- 2020/06/29 00:00 [received]
PHST- 2020/08/05 00:00 [revised]
PHST- 2020/08/09 00:00 [accepted]
PHST- 2020/08/16 06:00 [entrez]
PHST- 2020/08/17 06:00 [pubmed]
PHST- 2021/02/18 06:00 [medline]
PHST- 2020/08/01 00:00 [pmc-release]
AID - ijms21165749 [pii]
AID - ijms-21-05749 [pii]
AID - 10.3390/ijms21165749 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Aug 11;21(16):5749. doi: 10.3390/ijms21165749.