PMID- 3279712
OWN - NLM
STAT- MEDLINE
DCOM- 19880421
LR  - 20081121
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 32
IP  - 2
DP  - 1988 Mar-Apr
TI  - The value of the immunoperoxidase slide assay in the diagnosis of malignant 
      pleural effusions in breast cancer.
PG  - 188-92
AB  - Whether immunocytochemical studies of malignant pleural effusions due to breast 
      cancer would increase the diagnostic yield as compared with conventional effusion 
      cytology was examined in 30 cases with biopsy-proven metastatic spread to the 
      pleura. Conventional cytology was performed on air-dried smears as well as on 
      cytocentrifuge preparations stained with the May-Grunwald-Giemsa stain. 
      Immunocytochemistry was performed with monoclonal antibodies against 
      carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA) and human 
      leukocyte antigen (HLA) and the peroxidase-antiperoxidase technique on glass 
      slides after Ficoll-Hypaque centrifugation. By conventional cytology, 13 cases 
      (43%) were positive for malignant cells, 6 cases (20%) were suspicious, and 11 
      cases (37%) were negative. In marked contrast, all 30 cases were 
      immunocytologically positive for malignancy. Tumor cells in all cases 
      demonstrated a positive reaction for EMA. Some mesothelial cells were also 
      positive for EMA, but their reaction pattern was clearly distinguishable from 
      that of the tumor cells. Twenty-one cases (70%) also showed CEA-positive tumor 
      cells; mesothelial cells never reacted with CEA. Some tumor cells showed a loss 
      of HLA expression. In conclusion, this immunocytologic method can be recommended 
      as a routine procedure for greatly increasing the diagnostic yield of cytology in 
      pleural effusions due to breast cancer.
FAU - Guzman, J
AU  - Guzman J
AD  - Department of Pathology, University of Freiburg, West Germany.
FAU - Costabel, U
AU  - Costabel U
FAU - Bross, K J
AU  - Bross KJ
FAU - Wiehle, U
AU  - Wiehle U
FAU - Grunert, F
AU  - Grunert F
FAU - Schaefer, H E
AU  - Schaefer HE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (HLA Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Mucin-1)
SB  - IM
MH  - Breast Neoplasms/*pathology
MH  - Carcinoembryonic Antigen/analysis
MH  - Cytodiagnosis
MH  - HLA Antigens/analysis
MH  - Histocytochemistry
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Membrane Glycoproteins/analysis
MH  - Mucin-1
MH  - Pleural Effusion/*pathology
EDAT- 1988/03/01 00:00
MHDA- 1988/03/01 00:01
CRDT- 1988/03/01 00:00
PHST- 1988/03/01 00:00 [pubmed]
PHST- 1988/03/01 00:01 [medline]
PHST- 1988/03/01 00:00 [entrez]
PST - ppublish
SO  - Acta Cytol. 1988 Mar-Apr;32(2):188-92.