PMID- 32798494
OWN - NLM
STAT- MEDLINE
DCOM- 20220209
LR  - 20220209
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 114
DP  - 2021 Jan
TI  - Pro- and anti-inflammatory responses of common carp Cyprinus carpio head kidney 
      leukocytes to E.coli LPS as modified by different dietary plant oils.
PG  - 103828
LID - S0145-305X(20)30383-9 [pii]
LID - 10.1016/j.dci.2020.103828 [doi]
AB  - Dietary lipids could modify fatty acid (FA) composition in fish tissues. Long 
      chain polyunsaturated fatty acids (LC-PUFAs) such as arachidonic acid (ARA), 
      eicosapentaneoic acid (EPA) and docosahexaenoic acid (DHA) are able to modulate 
      the immune status in fish through an inflammatory process but their availability 
      may be limited when fish are exclusively fed plant oils. This study was conducted 
      to evaluate how to maximise the utilisation of dietary plant oil for an efficient 
      inflammatory response in common carp head kidney leukocytes (HKLs) exposed to a 
      gram-negative bacterial endotoxin, Escherichia coli lipopolysaccharides (LPS). 
      HKLs were isolated from fish fed cod liver oil (CLO), linseed oil (LO), sesame 
      oil (SO) a blend of SO and LO (SLO, v:v 1:1), and these plant oil diets 
      supplemented with DHA (SO + DHA, SOD) or ARA (LO + ARA, LOA) for 6 weeks. Cells 
      were then exposed to LPS at a dose of 10 mug/mL for 4 and 24 h. Peroxidase 
      activity, total Ig, and NO levels were measured in the culture medium, while 
      cells were used for expression analyses of candidate genes in pattern recognition 
      (tlr-4), eicosanoid metabolism (pge2, 5-lox), pro-inflammatory (il-1, il-6, il-8, 
      tnf-alpha, nf-kb, inos, cxc), anti-inflammatory (il-10, nf-kbi, tgf-beta1) responses, 
      and cytoprotective (gpx-1, prdx-3) processes. Results showed that LPS induced 
      significantly inflammatory responses, evidenced by a high level of almost all the 
      targeted humoral immune parameters and/or gene expression. Expression of 
      inflammatory cytokines and other inflammatory mediators was upregulated after 4 
      h-LPS exposure and reverted to basal levels after 24 h. HKLs from fish fed SLO, 
      LOA, or SOD diet exhibited a more efficient regulation of acute inflammatory 
      processes than those fed CLO diet. The results indicate that the immune 
      competence of fish fed plant oil mixture was comparable to the one of fish fed 
      fish oil diet. Moreover, the supplementation of ARA or DHA induced similar 
      immunomodulation in common carp.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Nguyen, Thi Mai
AU  - Nguyen TM
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium; Faculty 
      of Fisheries, Vietnam National University of Agriculture, Hanoi, Viet Nam. 
      Electronic address: ntmai.ntts@vnua.edu.vn.
FAU - Mandiki, Syaghalirwa N M
AU  - Mandiki SNM
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium.
FAU - Salomon, Jean M A J
AU  - Salomon JMAJ
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium.
FAU - Baruti, Joel Bondekwe
AU  - Baruti JB
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium.
FAU - Thi, Nang Thu Tran
AU  - Thi NTT
AD  - Faculty of Fisheries, Vietnam National University of Agriculture, Hanoi, Viet 
      Nam.
FAU - Nguyen, Thu Hang
AU  - Nguyen TH
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium; 
      Pharmacology Department, Hanoi University of Pharmacy, Hanoi, Viet Nam.
FAU - Nhu, Truong Quynh
AU  - Nhu TQ
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium; College 
      of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet 
      Nam.
FAU - Kestemont, Patrick
AU  - Kestemont P
AD  - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of 
      Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium. 
      Electronic address: Patrick.kestemont@unamur.be.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200813
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Cytokines)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Fish Proteins)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Oils)
SB  - IM
MH  - Animals
MH  - Carps/*immunology
MH  - Cells, Cultured
MH  - Cytokines/genetics/metabolism
MH  - Cytoprotection/genetics
MH  - Diet
MH  - Escherichia coli/*physiology
MH  - Escherichia coli Infections/*immunology
MH  - Fatty Acids, Unsaturated/metabolism
MH  - Fish Proteins/genetics/metabolism
MH  - Head Kidney/*immunology
MH  - Immunomodulation
MH  - Inflammation/*immunology
MH  - Inflammation Mediators/metabolism
MH  - Leukocytes/*immunology
MH  - Lipid Metabolism
MH  - Lipopolysaccharides/immunology
MH  - Plant Oils/*metabolism
OTO - NOTNLM
OT  - Cytokine
OT  - Eicosanoid
OT  - Inflammation
OT  - LC-PUFAs
OT  - NO activity
OT  - Peroxidase
OT  - Total Ig
EDAT- 2020/08/18 06:00
MHDA- 2022/02/10 06:00
CRDT- 2020/08/18 06:00
PHST- 2020/05/03 00:00 [received]
PHST- 2020/08/06 00:00 [revised]
PHST- 2020/08/06 00:00 [accepted]
PHST- 2020/08/18 06:00 [pubmed]
PHST- 2022/02/10 06:00 [medline]
PHST- 2020/08/18 06:00 [entrez]
AID - S0145-305X(20)30383-9 [pii]
AID - 10.1016/j.dci.2020.103828 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2021 Jan;114:103828. doi: 10.1016/j.dci.2020.103828. Epub 2020 
      Aug 13.