PMID- 32798558
OWN - NLM
STAT- MEDLINE
DCOM- 20201103
LR  - 20201103
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 259
DP  - 2020 Oct 15
TI  - Dexmedetomidine alleviates non-ventilation associated lung injury via modulating 
      immunology phenotypes of macrophages.
PG  - 118249
LID - S0024-3205(20)31001-8 [pii]
LID - 10.1016/j.lfs.2020.118249 [doi]
AB  - AIMS: We aimed to evaluate the effect of Dexmedetomidine (Dex) on immunology 
      function of macrophages and inflammatory reactions in non-ventilated lung tissues 
      from both humans and rats. MAIN METHODS: Patients scheduled for lung lobectomy 
      were randomly assigned to traditional anesthesia group or Dex anesthesia group, 
      15 subjects in each group. CD68, CD86 and CD206 were used to mark activate and 
      polarized macrophages using immunofluorescence staining in human lung tissues. 
      Sprague-Dawley rats were used to set lung injury model and randomly divided into 
      Control group, one-lung ventilation group (CLI group) and CLI + Dex group. Lung 
      tissues and bronchoalveolar lavage fluid (BALF) from non-ventilated lungs were 
      collected. The acquired lung tissues were subjected to hematoxylin-eosin (H&E) 
      staining and the inflammatory cells in BALF were calculated. Levels of cytokines 
      and chemokines were detected by enzyme-linked immunosorbent assays (ELISA). KEY 
      FINDINGS: Results from humans showed that anesthesia with Dex decreased the 
      number of both CD68 positive cells and CD86 positive cells and down-regulated 
      level of pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and monocyte 
      chemotactic protein 1 (MCP-1) in human lung. Results from rats demonstrated that 
      treatment with Dex reversed the increased inflammatory cells in lung and the 
      increased levels of TNF-alpha, interleukin-1beta (IL-beta), MCP-1 and chemokine (C-X-C 
      motif) ligand 1 (CXCL1) resulted from non-ventilation; Dex increased the 
      anti-inflammatory cytokine interleukin-10 (IL-10) in BALF from non-ventilated 
      lung. SIGNIFICANCE: This study showed that Dex modulated the activation and 
      immunological function of macrophages in non-ventilated lung and revealed a 
      protective role in collapsed lung injury.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Shen, Qiying
AU  - Shen Q
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China. Electronic address: shenqiying-yy@163.com.
FAU - Xu, Guanghong
AU  - Xu G
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China.
FAU - Liu, Jing
AU  - Liu J
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China; Department of Anesthesiology, the Fourth 
      Affiliated Hospital of Anhui Medical University, Hefei 230000, China.
FAU - Wang, Lixia
AU  - Wang L
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China.
FAU - Zhou, Yannan
AU  - Zhou Y
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China.
FAU - Yu, Yongqi
AU  - Yu Y
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China.
FAU - Lv, Changming
AU  - Lv C
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China.
FAU - Liu, Xuesheng
AU  - Liu X
AD  - Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei 230032, China. Electronic address: liuxuesheng@ahmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200813
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 67VB76HONO (Dexmedetomidine)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid
MH  - Cytokines/metabolism
MH  - Dexmedetomidine/metabolism/*pharmacology
MH  - Female
MH  - Humans
MH  - Lung/cytology/metabolism
MH  - Lung Injury/*drug therapy/pathology
MH  - Macrophages/*drug effects/metabolism
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/pathology
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 4/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Dexmedetomidine
OT  - Inflammation
OT  - Lung injury
OT  - Macrophages
OT  - One-lung ventilation
COIS- Declaration of competing interest The authors have declared that they have no 
      competing interest.
EDAT- 2020/08/18 06:00
MHDA- 2020/11/04 06:00
CRDT- 2020/08/18 06:00
PHST- 2020/05/22 00:00 [received]
PHST- 2020/07/29 00:00 [revised]
PHST- 2020/08/07 00:00 [accepted]
PHST- 2020/08/18 06:00 [pubmed]
PHST- 2020/11/04 06:00 [medline]
PHST- 2020/08/18 06:00 [entrez]
AID - S0024-3205(20)31001-8 [pii]
AID - 10.1016/j.lfs.2020.118249 [doi]
PST - ppublish
SO  - Life Sci. 2020 Oct 15;259:118249. doi: 10.1016/j.lfs.2020.118249. Epub 2020 Aug 
      13.