PMID- 32803119
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231111
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 18
IP  - 7
DP  - 2020 Jul
TI  - Impact of coadministration of apigenin and bone marrow stromal cells on damaged 
      ovaries due to chemotherapy in rat: An experimental study.
PG  - 551-560
LID - 10.18502/ijrm.v13i7.7372 [doi]
AB  - BACKGROUND: Apigenin is a plant-derived flavonoid with antioxidative and 
      antiapoptotic effects. Bone marrow stromal cells (BMSCs) are a type of 
      mesenchymal stem cells (MSCs) that may recover damaged ovaries. It seems that 
      apigenin may promote the differentiation of MSCs. OBJECTIVE: The aim of this 
      study was to investigate the effect of coadministration of apigenin and BMSCs on 
      the function, structure, and apoptosis of the damaged ovaries after creating a 
      chemotherapy model with cyclophosphamide in rat. MATERIALS AND METHODS: For 
      chemotherapy induction and ovary destruction, cyclophosphamide was injected 
      intraperitoneally to 40 female Wistar rats (weighing 180-200 gr, 10 wk old) for 
      14 days. Then, the rats were randomly divided into four groups (n = 10/each): 
      control, apigenin, BMSCs and coadministration of apigenin and BMSCs. Injection of 
      apigenin was performed intraperitoneally and BMSC transplantation was performed 
      locally in the ovaries. The level of anti-mullerian hormone serum by ELISA kit, 
      the number of oocytes by superovulation, the number of ovarian follicles in 
      different stages by H&E staining, and the expression of ovarian Bcl-2 and Bax 
      proteins by western blot were assessed after four wk. RESULTS: The results of 
      serum anti-mullerian hormone level, number of oocytes and follicles, and 
      Bcl-2/Bax expression ratio showed that coadministration of apigenin and BMSCs 
      significantly recovered the ovarian function, structure, and apoptosis compared 
      to the control, BMSC, and apigenin groups (p  <  0.001). CONCLUSION: The results 
      suggest that the effect of coadministration of apigenin and BMSCs is maybe more 
      effective than the effect of their administrations individually on the recovery 
      of damaged ovaries following the chemotherapy with cyclophosphamide in rats.
CI  - Copyright (c) 2020 Talebi et al.
FAU - Talebi, Athar
AU  - Talebi A
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Hayati Roodbari, Nasim
AU  - Hayati Roodbari N
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Reza Sameni, Hamid
AU  - Reza Sameni H
AD  - Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, 
      Semnan, Iran.
FAU - Zarbakhsh, Sam
AU  - Zarbakhsh S
AD  - Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, 
      Semnan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200722
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC7385912
OTO - NOTNLM
OT  - Bone marrow stromal cells
OT  - Chemotherapy
OT  - Regeneration.
OT  - Apigenin
OT  - Ovary
COIS- There is no conflict of interest in this article.
EDAT- 2020/08/18 06:00
MHDA- 2020/08/18 06:01
PMCR- 2020/07/22
CRDT- 2020/08/18 06:00
PHST- 2019/07/10 00:00 [received]
PHST- 2019/09/04 00:00 [revised]
PHST- 2019/12/30 00:00 [accepted]
PHST- 2020/08/18 06:00 [entrez]
PHST- 2020/08/18 06:00 [pubmed]
PHST- 2020/08/18 06:01 [medline]
PHST- 2020/07/22 00:00 [pmc-release]
AID - 10.18502/ijrm.v13i7.7372 [doi]
PST - epublish
SO  - Int J Reprod Biomed. 2020 Jul 22;18(7):551-560. doi: 10.18502/ijrm.v13i7.7372. 
      eCollection 2020 Jul.