PMID- 32803763
OWN - NLM
STAT- MEDLINE
DCOM- 20210830
LR  - 20211204
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 236
IP  - 1
DP  - 2021 Jan
TI  - Long noncoding RNA NEAT1 promotes progression of glioma as a ceRNA by sponging 
      miR-185-5p to stimulate DNMT1/mTOR signaling.
PG  - 121-130
LID - 10.1002/jcp.29644 [doi]
AB  - Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) is regarded 
      as an oncogene in multiple cancers. Previous studies have shown that NEAT1 is 
      involved in the proliferation and tumorigenesis of glioma cells, while miR-185-5p 
      functions as a tumor suppressor in glioma. However, the underlying molecular 
      mechanism of NEAT1 in glioma, especially in association with miR-185-5p, has not 
      been studied. In this study, we first demonstrated that NEAT1 expression was 
      upregulated, and miR-185-5p downregulated in glioma tissues and cells. More 
      important, NEAT1 expression was negatively correlated with miR-185-5p expression 
      in glioma tissues. In vitro and in vivo experiments verified that NEAT1 was a 
      competing endogenous RNA for miR-185-5p for promoting DNA methyltransferase 1 
      (DNMT1) expression and activated mammalian target of rapamycin (mTOR) signaling, 
      thus inhibiting apoptosis, and promoting glioma migration, proliferation, and 
      epithelial-mesenchymal transition process. Furthermore, NEAT1 knockdown 
      suppressed tumor growth and reduced the expression of proliferation antigen 
      Ki-67, DNMT1, and mTOR, but upregulated the expression of miR-185-5p in vivo. 
      Finally, with mTOR inhibitor rapamycin, we confirmed that NEAT1 promoted glioma 
      activity through mTOR signaling both in vitro and in vivo. In conclusion, these 
      results suggest that NEAT1 promotes glioma tumorigenesis via 
      miR-185-5p/DNMT1/mTOR signaling, which may provide a new target for the diagnosis 
      and therapy of glioma.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Yu, Heng
AU  - Yu H
AD  - Department of Clinical Laboratory, Chengdu Women's and Children's Central 
      Hospital, School of Medicine, University of Electronic Science and Technology of 
      China, Chengdu, China.
FAU - Xu, Anchun
AU  - Xu A
AD  - Department of Clinical Laboratory, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Wu, Bo
AU  - Wu B
AD  - Department of Clinical Laboratory, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, China.
FAU - Wang, Meng
AU  - Wang M
AUID- ORCID: 0000-0002-5909-6014
AD  - Department of Rehabilitation, Huai'an Second People's Hospital, The Affiliated 
      Huai'an Hospital of Xuzhou Medical University, Huai'an, China.
FAU - Chen, Zhongjun
AU  - Chen Z
AUID- ORCID: 0000-0003-4148-1683
AD  - Department of Neurosurgery, Huai'an Second People's Hospital, The Affiliated 
      Huai'an Hospital of Xuzhou Medical University, Huai'an, China.
LA  - eng
PT  - Journal Article
DEP - 20200816
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (MIRN185 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NEAT1 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cell Transformation, Neoplastic/genetics/pathology
MH  - DNA (Cytosine-5-)-Methyltransferase 1/*genetics
MH  - Disease Progression
MH  - Down-Regulation/genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Glioma/*genetics/pathology
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction/*genetics
MH  - TOR Serine-Threonine Kinases/*genetics
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - DNMT1
OT  - NEAT1
OT  - ceRNA
OT  - glioma
OT  - miR-185-5p
EDAT- 2020/08/18 06:00
MHDA- 2021/08/31 06:00
CRDT- 2020/08/18 06:00
PHST- 2019/07/03 00:00 [received]
PHST- 2020/02/04 00:00 [accepted]
PHST- 2020/08/18 06:00 [pubmed]
PHST- 2021/08/31 06:00 [medline]
PHST- 2020/08/18 06:00 [entrez]
AID - 10.1002/jcp.29644 [doi]
PST - ppublish
SO  - J Cell Physiol. 2021 Jan;236(1):121-130. doi: 10.1002/jcp.29644. Epub 2020 Aug 
      16.