PMID- 32807030
OWN - NLM
STAT- MEDLINE
DCOM- 20210111
LR  - 20210818
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 32
IP  - 6
DP  - 2020 Nov
TI  - Serum paraoxonase 1 activity in cats: analytical validation, reference intervals, 
      and correlation with serum amyloid A and alpha-1-acid glycoprotein.
PG  - 844-855
LID - 10.1177/1040638720949638 [doi]
AB  - Paraoxonase 1 (PON1) is an inflammation marker associated with lipid oxidation 
      and is used as a diagnostic marker in people. There is no information about the 
      suitable substrate and analytic performance in cats, or its biological behavior 
      compared with other inflammation markers. Our aims were to validate a 
      paraoxon-based method to measure PON1 activity in feline serum, to assess 
      stability of PON1 under different storage conditions and the impact of 
      interfering elements, to determine a reference interval (RI) for healthy cats, 
      and to correlate PON1 activity with 2 major acute-phase proteins. Intra- and 
      inter-assay precision, accuracy, and RI were assessed using fresh serum. The same 
      specimens were stored at room temperature, refrigerated, or frozen, and retested 
      at defined intervals. Hemolysis, lipemia, and icterus were simulated to study 
      interferences. PON1 results were compared to serum amyloid A (SAA) and 
      alpha-1-acid glycoprotein (AGP) results. Analytical validation yielded precise 
      and accurate results. PON1 activity is stable for up to 24 h at room temperature 
      and up to 48 h at 4 degrees C. Freezing at -20 degrees C results in an increase after 72 h, with 
      return to baseline values after 1 wk, that again increases after 6 mo. Only 
      hyperlipemia interfered with PON1 activity. The RI based on 71 healthy cats was 
      58-154 U/L. PON1 activity was negatively correlated with AGP, but not with SAA. 
      Serum PON1 activity can be measured accurately in cats, and it acts as a negative 
      acute-phase protein.
FAU - Rossi, Gabriele
AU  - Rossi G
AUID- ORCID: 0000-0003-4879-9504
AD  - College of Veterinary Medicine, School of Veterinary and Life Science, Murdoch 
      University, Perth, Australia.
FAU - Meazzi, Sara
AU  - Meazzi S
AUID- ORCID: 0000-0002-8157-7973
AD  - Department of Veterinary Medicine and Veterinary Teaching Hospital, University of 
      Milan, Lodi, Italy.
FAU - Giordano, Alessia
AU  - Giordano A
AUID- ORCID: 0000-0002-8611-8944
AD  - Department of Veterinary Medicine and Veterinary Teaching Hospital, University of 
      Milan, Lodi, Italy.
FAU - Paltrinieri, Saverio
AU  - Paltrinieri S
AUID- ORCID: 0000-0001-7117-7987
AD  - Department of Veterinary Medicine and Veterinary Teaching Hospital, University of 
      Milan, Lodi, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200817
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Biomarkers)
RN  - 0 (Orosomucoid)
RN  - 0 (Serum Amyloid A Protein)
RN  - EC 3.1.8.1 (Aryldialkylphosphatase)
SB  - IM
MH  - Animals
MH  - Aryldialkylphosphatase/*blood/metabolism
MH  - Biomarkers/blood
MH  - Cat Diseases/*blood
MH  - Cats
MH  - Female
MH  - Humans
MH  - Male
MH  - Orosomucoid/*metabolism
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Serum Amyloid A Protein/*metabolism
PMC - PMC7649547
OTO - NOTNLM
OT  - acute-phase protein
OT  - assay interference
OT  - feline
OT  - inflammation
OT  - serum paraoxonase-1 activity
OT  - storage
COIS- Declaration of conflicting interests: The authors declared no potential conflicts 
      of interest with respect to the research, authorship, and/or publication of this 
      article.
EDAT- 2020/08/19 06:00
MHDA- 2021/01/12 06:00
PMCR- 2021/08/17
CRDT- 2020/08/19 06:00
PHST- 2020/08/19 06:00 [pubmed]
PHST- 2021/01/12 06:00 [medline]
PHST- 2020/08/19 06:00 [entrez]
PHST- 2021/08/17 00:00 [pmc-release]
AID - 10.1177_1040638720949638 [pii]
AID - 10.1177/1040638720949638 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2020 Nov;32(6):844-855. doi: 10.1177/1040638720949638. Epub 
      2020 Aug 17.