PMID- 32808226
OWN - NLM
STAT- MEDLINE
DCOM- 20210318
LR  - 20210318
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2184
DP  - 2020
TI  - Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by 
      Assessing Their Oxygen Consumption and Extracellular Acidification.
PG  - 185-196
LID - 10.1007/978-1-0716-0802-9_13 [doi]
AB  - Dendritic cells (DCs) are the bridge between innate and T cell-dependent adaptive 
      immunity, and are promising therapeutic targets for cancer and immune-mediated 
      disorders. In the recent past, DCs have gained significant interest to manipulate 
      them for the treatment of cancer and immune-mediated disorders. This can be 
      achieved by differentiating them into either immunogenic or tolerogenic DCs 
      (TolDCs), by modulating their metabolic pathways, including glycolysis, oxidative 
      phosphorylation, and fatty acid metabolism, to orchestrate their desired 
      function. For immunogenic DCs, this maturation shifts the metabolic profile to a 
      glycolytic metabolic state and leads to the use of glucose as a carbon source, 
      whereas TolDCs prefer oxidative phosphorylation (OXPHOS) and fatty acid oxidation 
      for their energy resource.Understanding the metabolic regulation of DC subsets 
      and functions at large not only will improve our understanding of DC biology and 
      immune regulation, but can also open up opportunities for treating 
      immune-mediated ailments and cancers by tweaking endogenous T-cell responses 
      through DC-based immunotherapies. Here we describe a method to analyze this 
      dichotomous metabolic reprogramming of the DCs for generating reliable and 
      effective DC cell therapy products. We, hereby, report how to measure the OXPHOS 
      and glycolysis level of DCs. We focus on the metabolic reprogramming of TolDCs 
      using a pharmacological nuclear factor (erythroid-derived 2)-like-2 factor (Nrf2) 
      activator as an example to illustrate the metabolic profile of TolDCs.
FAU - Wei, Hsi-Ju
AU  - Wei HJ
AD  - Department of Biochemistry, School of Medicine, Case Western Reserve University, 
      Cleveland, OH, USA.
AD  - The Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, 
      OH, USA.
FAU - Letterio, John J
AU  - Letterio JJ
AD  - The Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, 
      OH, USA. John.Letterio@uhhospitals.org.
AD  - Division of Pediatric Hematology/Oncology, Department of Pediatrics, Case Western 
      Reserve University, Cleveland, OH, USA. John.Letterio@uhhospitals.org.
AD  - Angie Fowler Cancer Institute, Rainbow Babies and Children's Hospital, University 
      Hospitals, Cleveland, OH, USA. John.Letterio@uhhospitals.org.
AD  - Celloram Inc., Cleveland, OH, USA. John.Letterio@uhhospitals.org.
FAU - Pareek, Tej K
AU  - Pareek TK
AD  - The Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, 
      OH, USA. tkp5@case.edu.
AD  - Division of Pediatric Hematology/Oncology, Department of Pediatrics, Case Western 
      Reserve University, Cleveland, OH, USA. tkp5@case.edu.
AD  - Angie Fowler Cancer Institute, Rainbow Babies and Children's Hospital, University 
      Hospitals, Cleveland, OH, USA. tkp5@case.edu.
AD  - Celloram Inc., Cleveland, OH, USA. tkp5@case.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fatty Acids)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 7440-44-0 (Carbon)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Bone Marrow/*metabolism
MH  - Carbon/metabolism
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - Fatty Acids/metabolism
MH  - Glucose/metabolism
MH  - Glycolysis/physiology
MH  - Immune Tolerance/physiology
MH  - Metabolic Networks and Pathways/physiology
MH  - Mice
MH  - NF-E2-Related Factor 2/metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Phosphorylation
MH  - Oxygen Consumption/*physiology
MH  - Phenotype
MH  - T-Lymphocytes/metabolism
OTO - NOTNLM
OT  - Dendritic cells
OT  - Glycolysis
OT  - Immunometabolism
OT  - Oxidative phosphorylation
EDAT- 2020/08/19 06:00
MHDA- 2021/03/19 06:00
CRDT- 2020/08/19 06:00
PHST- 2020/08/19 06:00 [entrez]
PHST- 2020/08/19 06:00 [pubmed]
PHST- 2021/03/19 06:00 [medline]
AID - 10.1007/978-1-0716-0802-9_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2184:185-196. doi: 10.1007/978-1-0716-0802-9_13.