PMID- 3280948
OWN - NLM
STAT- MEDLINE
DCOM- 19880428
LR  - 20061115
IS  - 0391-5352 (Print)
IS  - 0391-5352 (Linking)
VI  - 11
IP  - 1
DP  - 1988 Jan
TI  - Kinetics of phagocytosis and killing of E. coli by murine macrophages in presence 
      of different serum preparations.
PG  - 13-20
AB  - In this study we evaluated the kinetics of phagocytosis and killing of E. coli by 
      thioglycollate-elicited murine peritoneal macrophages and the role of specific 
      antibodies and complement present in different serum preparations in modulating 
      these processes. In our system phagocytosis of E. coli by macrophage monolayer 
      was exponential for 180 min. The killing activity was high in the first 30-60 min 
      and then virtually ceased. The least phagocytosis and killing occurred in 
      presence of heat-inactivated fetal calf serum (HFCS). These activities were 
      2-fold increased in presence of normal mouse serum (NMS) or heat-inactivated 
      newborn calf serum (HNCS) and were highly stimulated in presence of immune mouse 
      serum (IMS). IMS without complement was less efficient in enhancing phagocytosis 
      and killing by macrophages. However when IMS or HNCS were deprived of specific 
      antibodies their activity was remarkably reduced. When macrophages containing 
      phagocytized bacteria were reincubated with different sera, multiplication of 
      intracellular E. coli occurred with HFCS, NMS or antibody-deprived IMS or HNCS. 
      In contrast, a significant decrease in the survival of intracellular bacteria was 
      seen in presence of IMS, HNCS or complement-deprived IMS. The results indicated 
      that specific bacterial antibodies play a major role in the phagocytic process 
      and in the activation of killing mechanisms. However optimal macrophage activity 
      resulted from the presence of both specific antibodies and complement.
FAU - Adinolfi, L E
AU  - Adinolfi LE
AD  - Cattedra di Terapia Medica Sistematica, I Facolta di Medicina e Chirurgia, 
      Universita di Napoli, Italy.
FAU - Dilillo, M
AU  - Dilillo M
FAU - Tripodi, M F
AU  - Tripodi MF
FAU - Utili, R
AU  - Utili R
FAU - Bonventre, P F
AU  - Bonventre PF
FAU - Ruggiero, G
AU  - Ruggiero G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Microbiologica
JT  - Microbiologica
JID - 7902903
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immune Sera)
RN  - 0 (Opsonin Proteins)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/*immunology
MH  - Complement System Proteins/*immunology
MH  - Escherichia coli/*immunology
MH  - Female
MH  - Immune Sera/immunology
MH  - Kinetics
MH  - Macrophages/*immunology
MH  - Mice
MH  - Opsonin Proteins/immunology
MH  - Peritoneal Cavity/cytology
MH  - *Phagocytosis
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PST - ppublish
SO  - Microbiologica. 1988 Jan;11(1):13-20.