PMID- 32810790
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 157
DP  - 2020 Nov
TI  - Shotgun proteome analysis of seminal plasma differentiate boars by reproductive 
      performance.
PG  - 130-139
LID - S0093-691X(20)30410-6 [pii]
LID - 10.1016/j.theriogenology.2020.07.013 [doi]
AB  - There is a need to identify subfertile boars before they enter into the breeding 
      herd. Seminal plasma proteins are essential for normal sperm function and 
      transport and play an important role in fertilization. The objective of this 
      study was to use liquid chromatography tandem mass spectrometry for shotgun 
      proteome analysis to investigate whether differences in boar fertility phenotype 
      can be differentiated by seminal plasma protein abundance. Following 50 
      breedings, boars were categorized into one of four phenotypes: high farrowing 
      rate and total born (HFHB; n = 9), high farrowing rate with low total born (HFLB; 
      n = 10), low farrowing rate and total born (LFLB; n = 9), and low farrowing rate 
      with high total born (LFHB; n = 4) that were distinct (p < 0.05) from each other 
      by these variables. There were 506 proteins measured in at least one sample 
      across all animals. There were 245 high confidence proteins and 56 were 
      differentially abundant between the high fertility phenotype (HFHB) and at least 
      one of the three subfertile groups. Findings support that seminal plasma protein 
      profiles are distinct between boars with different fertility phenotypes.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Mills, Kayla M
AU  - Mills KM
AD  - Department of Animal Science, Purdue University, 270 S Russell Street, West 
      Lafayette, IN, 47907, USA. Electronic address: mills34@purdue.edu.
FAU - Aryal, Uma K
AU  - Aryal UK
AD  - Purdue Proteomics Facility, Bindley Bioscience Center, Purdue University, 1275 
      3rd Street, West Lafayette, IN, 47906, USA; Department of Comparative 
      Pathobiology, Purdue University, 625 Harrison Street, West Lafayette, IN, 47907, 
      USA. Electronic address: uaryal@purdue.edu.
FAU - Sobreira, Tiago
AU  - Sobreira T
AD  - Purdue Proteomics Facility, Bindley Bioscience Center, Purdue University, 1275 
      3rd Street, West Lafayette, IN, 47906, USA. Electronic address: 
      sobreira@purdue.edu.
FAU - Minton, Amanda M
AU  - Minton AM
AD  - The Maschhoff's LLC, 7475 St. Rte. 127, Carlyle, IL, 62231, USA. Electronic 
      address: amanda.minton@pigsrus.net.
FAU - Casey, Theresa
AU  - Casey T
AD  - Department of Animal Science, Purdue University, 270 S Russell Street, West 
      Lafayette, IN, 47907, USA. Electronic address: theresa-casey@purdue.edu.
FAU - Stewart, Kara R
AU  - Stewart KR
AD  - Department of Animal Science, Purdue University, 270 S Russell Street, West 
      Lafayette, IN, 47907, USA. Electronic address: krstewart@purdue.edu.
LA  - eng
PT  - Journal Article
DEP - 20200726
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Proteome)
RN  - 0 (Seminal Plasma Proteins)
SB  - IM
MH  - Animals
MH  - Fertility
MH  - Male
MH  - *Proteome
MH  - *Semen
MH  - Seminal Plasma Proteins
MH  - Spermatozoa
MH  - Swine
OTO - NOTNLM
OT  - Fertility
OT  - Pig
OT  - Proteomics
OT  - Seminal plasma
EDAT- 2020/08/19 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/08/19 06:00
PHST- 2020/03/11 00:00 [received]
PHST- 2020/06/17 00:00 [revised]
PHST- 2020/07/09 00:00 [accepted]
PHST- 2020/08/19 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/08/19 06:00 [entrez]
AID - S0093-691X(20)30410-6 [pii]
AID - 10.1016/j.theriogenology.2020.07.013 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Nov;157:130-139. doi: 10.1016/j.theriogenology.2020.07.013. 
      Epub 2020 Jul 26.