PMID- 32813865
OWN - NLM
STAT- MEDLINE
DCOM- 20201005
LR  - 20201005
IS  - 1678-2690 (Electronic)
IS  - 0001-3765 (Linking)
VI  - 92
IP  - 2
DP  - 2020
TI  - Type 2 diabetes mellitus alters cardiac mitochondrial content and function in a 
      non-obese mice model.
PG  - e20191340
LID - S0001-37652020000300801 [pii]
LID - 10.1590/0001-3765202020191340 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is associated with an increase of premature 
      appearance of several disorders such as cardiac complications. Thus, we test the 
      hypothesis that a combination of a high fat diet (HFD) and low doses of 
      streptozotocin (STZ) recapitulate a suitable mice model of T2DM to study the 
      cardiac mitochondrial disturbances induced by this disease. Animals were divided 
      in 2 groups: the T2DM group was given a HFD and injected with 2 low doses of STZ, 
      while the CNTRL group was given a standard chow and a buffer solution. The 
      combination of HFD and STZ recapitulate the T2DM metabolic profile showing higher 
      blood glucose levels in T2DM mice when compared to CNTRL, and also, insulin 
      resistance. The kidney structure/function was preserved. Regarding cardiac 
      mitochondrial function, in all phosphorylative states, the cardiac mitochondria 
      from T2DM mice presented reduced oxygen fluxes when compared to CNTRL mice. Also, 
      mitochondria from T2DM mice showed decreased citrate synthase activity and lower 
      protein content of mitochondrial complexes. Our results show that in this 
      non-obese T2DM model, which recapitulates the classical metabolic alterations, 
      mitochondrial function is impaired and provides a useful model to deepen study 
      the mechanisms underlying these alterations.
FAU - Yurre, Ainhoa R DE
AU  - Yurre AR
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Martins, Eduarda G L
AU  - Martins EGL
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Lopez-Alarcon, Micaela
AU  - Lopez-Alarcon M
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Cabral, Bruno
AU  - Cabral B
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Vera, Narendra
AU  - Vera N
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Lopes, Jarlene A
AU  - Lopes JA
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Galina, Antonio
AU  - Galina A
AD  - Instituto de Bioquimica Medica Leopoldo de Meis, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Takiya, Christina M
AU  - Takiya CM
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Lindoso, Rafael S
AU  - Lindoso RS
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Vieyra, Adalberto
AU  - Vieyra A
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - SAenz, Oscar C
AU  - SAenz OC
AD  - Faculdade de Farmacia, Universidade do Pais Vasco (UPV-EHU), Vitoria-Gasteiz, 
      Spain.
FAU - Medei, Emiliano
AU  - Medei E
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20200812
PL  - Brazil
TA  - An Acad Bras Cienc
JT  - Anais da Academia Brasileira de Ciencias
JID - 7503280
RN  - 0 (Blood Glucose)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - *Diabetes Mellitus, Type 2
MH  - Insulin Resistance
MH  - Mice
MH  - Mitochondria
MH  - Streptozocin
EDAT- 2020/08/20 06:00
MHDA- 2020/10/06 06:00
CRDT- 2020/08/20 06:00
PHST- 2019/04/30 00:00 [received]
PHST- 2020/03/06 00:00 [accepted]
PHST- 2020/08/20 06:00 [entrez]
PHST- 2020/08/20 06:00 [pubmed]
PHST- 2020/10/06 06:00 [medline]
AID - S0001-37652020000300801 [pii]
AID - 10.1590/0001-3765202020191340 [doi]
PST - ppublish
SO  - An Acad Bras Cienc. 2020;92(2):e20191340. doi: 10.1590/0001-3765202020191340. 
      Epub 2020 Aug 12.