PMID- 32813896
OWN - NLM
STAT- MEDLINE
DCOM- 20210413
LR  - 20210413
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 130
IP  - 3
DP  - 2021 Mar
TI  - Effects of the prebiotic inulin-type fructans on post-antibiotic reconstitution 
      of the gut microbiome.
PG  - 634-649
LID - 10.1111/jam.14827 [doi]
AB  - AIMS: Interventions using prebiotic inulin-type fructans (ITFs) are widely 
      prescribed to modulate the gut microbiota composition and activity to promote 
      health. However, the impacts of ITFs on post-antibiotic reconstitution of the gut 
      microbiome remain incompletely understood. The aim of the present study was to 
      investigate the effects of ITFs supplementation on intestinal inflammation, the 
      composition of the intestinal microbiota and the colonic transcriptome after 
      antibiotic treatment. METHODS AND RESULTS: Male BALB/c mice were subjected to an 
      antibiotic cocktail (ABx) treatment for 7 days, and their microbiomes were then 
      reconstituted either spontaneously or with ITFs supplementation (5%) for 14 days. 
      Our data showed that ITFs supplementation delayed the recovery of 
      antibiotic-induced colitis compared with the spontaneous recovery. Neither ITFs 
      supplementation nor spontaneous recovery could restore the microbial community 
      composition at the genus level back to its initial composition. ITFs 
      supplementation increased the relative abundance of some beneficial bacteria and 
      butyrate levels, but resulted in selective blooms of some opportunistic pathogens 
      and elevated the pathways associated with diseases linked to gut microbiota 
      function. Both ITFs supplementation and spontaneous recovery could restore the 
      colonic transcriptome nearly to the initial profile to a certain extent; however, 
      ITFs supplementation delayed the restoration of the immunoglobulin genes compared 
      to spontaneous recovery. CONCLUSION: These data showed that post-antibiotic ITFs 
      consumption did not always lead to beneficial effects but might lead to potential 
      adverse effects in the context of dysbiosis. SIGNIFICANCE AND IMPACT OF THE 
      STUDY: These findings highlighted that caution is required when supplementing 
      ITFs to restore intestinal homeostasis in the context of dysbiosis resulting from 
      broad-spectrum antibiotics.
CI  - (c) 2020 The Society for Applied Microbiology.
FAU - Tao, C
AU  - Tao C
AUID- ORCID: 0000-0003-0398-9646
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Zeng, W
AU  - Zeng W
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Zhang, Q
AU  - Zhang Q
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Liu, G
AU  - Liu G
AUID- ORCID: 0000-0002-7100-9411
AD  - College of Light Industry and Food Science, Zhongkai University of Agriculture 
      and Engineering, Guangzhou, China.
FAU - Wu, F
AU  - Wu F
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Shen, H
AU  - Shen H
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Zhang, W
AU  - Zhang W
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Bo, H
AU  - Bo H
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
FAU - Shao, H
AU  - Shao H
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 
      China.
LA  - eng
GR  - 81703053/the National Natural Science Foundation of China/
GR  - 2018B020206001/Guangdong Province Science and Technology Innovation Strategy 
      Special Fund/
GR  - 2019KJ101/Guangdong Provincial Agricultural Science and Technology Innovation and 
      Extension Project in 2019/
GR  - 2017KZDXM049/Innovative and Strong School Project of Guangdong Higher Education 
      Institutions/
GR  - 2017KCXTD020/Innovative and Strong School Project of Guangdong Higher Education 
      Institutions/
PT  - Journal Article
DEP - 20200903
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fructans)
RN  - 0 (Prebiotics)
RN  - 9005-80-5 (Inulin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Bacteria/isolation & purification
MH  - Colitis/chemically induced/genetics/microbiology
MH  - Colon/metabolism
MH  - Fructans/*pharmacology
MH  - Gastrointestinal Microbiome/*drug effects
MH  - Homeostasis/drug effects
MH  - Inulin
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Prebiotics
MH  - Transcriptome/drug effects
OTO - NOTNLM
OT  - antibiotic cocktail (ABx)
OT  - gut microbiota
OT  - inulin-type fructans (ITFs)
OT  - prebiotic
OT  - reconstitution
EDAT- 2020/08/20 06:00
MHDA- 2021/04/14 06:00
CRDT- 2020/08/20 06:00
PHST- 2020/05/03 00:00 [received]
PHST- 2020/08/14 00:00 [revised]
PHST- 2020/08/17 00:00 [accepted]
PHST- 2020/08/20 06:00 [pubmed]
PHST- 2021/04/14 06:00 [medline]
PHST- 2020/08/20 06:00 [entrez]
AID - 10.1111/jam.14827 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2021 Mar;130(3):634-649. doi: 10.1111/jam.14827. Epub 2020 Sep 
      3.