PMID- 32813917
OWN - NLM
STAT- MEDLINE
DCOM- 20210728
LR  - 20210728
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 56
IP  - 2
DP  - 2021 Feb
TI  - Recovery of sperms bearing X chromosomes with different concentrations of 
      magnetic nanoparticles in ram.
PG  - 263-269
LID - 10.1111/rda.13807 [doi]
AB  - Pre-conceptual sex selection is still a highly debatable process whereby X and Y 
      chromosome bearing spermatozoa are isolated before oocyte fertilization. 
      Recently, magnetic nanoparticles (MNP) have been used to determine X and Y 
      chromosomes bearing spermatozoa as a result of searching for a cheap, highly 
      efficient method using non-toxic materials. This study aimed to recover the sperm 
      bearing X chromosomes in ram with different concentrations of MNP and then 
      evaluate the success of this method using polymerase chain reaction (PCR). Ram 
      sperms were divided into four groups, treated with 0 (control), 50, 100 and 
      200 mug/ml MNP, respectively. MNP was used to restore sperm cells bearing X 
      chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, 
      Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and 
      acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed 
      that the treatment of spermatozoa- bearing X chromosomes with 50 mug/ml MNP had 
      the highest effects on the recovery of X sperm rather than the other 
      concentrations of MNP. However, the concentrations of MNP did not have any toxic 
      effects on spermatozoa, sperm viability and, DNA integrity, but the high 
      concentration of MNP (200 mug/ml) significantly reduced DNA integrity. According 
      to MTT and AO results, the concentrations of MNP used in this study had no toxic 
      effects on spermatozoa and did not reduce the sperm viability and DNA integrity, 
      except that 200 mug/ml MNP significantly reduced DNA integrity.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Moradi, Maryam
AU  - Moradi M
AD  - Department of Animal Science, Faculty of Agricultural and Engineering Science, 
      Razi University, Kermanshah, Iran.
FAU - Hajarian, Hadi
AU  - Hajarian H
AUID- ORCID: 0000-0003-0738-429X
AD  - Department of Animal Science, Faculty of Agricultural and Engineering Science, 
      Razi University, Kermanshah, Iran.
FAU - Karamishabankareh, Hamed
AU  - Karamishabankareh H
AUID- ORCID: 0000-0002-4810-2415
AD  - Department of Animal Science, Faculty of Agricultural and Engineering Science, 
      Razi University, Kermanshah, Iran.
FAU - Soltani, Leila
AU  - Soltani L
AD  - Department of Animal Science, Faculty of Agricultural and Engineering Science, 
      Razi University, Kermanshah, Iran.
FAU - Soleymani, Bijan
AU  - Soleymani B
AUID- ORCID: 0000-0003-3059-413X
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Science, Kermanshah, Iran.
LA  - eng
PT  - Journal Article
DEP - 20201222
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Magnetite Nanoparticles)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - DNA Damage/drug effects
MH  - Magnetite Nanoparticles/*chemistry/toxicity
MH  - Male
MH  - Sex Preselection/methods/*veterinary
MH  - Sheep
MH  - Sperm Motility/drug effects
MH  - *Spermatozoa
MH  - *X Chromosome
OTO - NOTNLM
OT  - PCR method
OT  - magnetic nanoparticles
OT  - ram
OT  - semen
OT  - sperm recovery
EDAT- 2020/08/20 06:00
MHDA- 2021/07/29 06:00
CRDT- 2020/08/20 06:00
PHST- 2020/03/02 00:00 [received]
PHST- 2020/07/28 00:00 [revised]
PHST- 2020/08/15 00:00 [accepted]
PHST- 2020/08/20 06:00 [pubmed]
PHST- 2021/07/29 06:00 [medline]
PHST- 2020/08/20 06:00 [entrez]
AID - 10.1111/rda.13807 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2021 Feb;56(2):263-269. doi: 10.1111/rda.13807. Epub 2020 Dec 
      22.