PMID- 32816959
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1477-9145 (Electronic)
IS  - 0022-0949 (Print)
IS  - 0022-0949 (Linking)
VI  - 223
IP  - Pt 20
DP  - 2020 Oct 15
TI  - High temperature induces transcriptomic changes in Crassostrea gigas that hinder 
      progress of ostreid herpesvirus (OsHV-1) and promote survival.
LID - 10.1242/jeb.226233 [doi]
LID - jeb226233
AB  - Of all environmental factors, seawater temperature plays a decisive role in 
      triggering marine diseases. Like fever in vertebrates, high seawater temperature 
      could modulate the host response to pathogens in ectothermic animals. In France, 
      massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid 
      herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24 degrees C in the 
      field. In the present study we assess how high temperature influences the host 
      response to the pathogen by comparing transcriptomes (RNA sequencing) during the 
      course of experimental infection at 21 degrees C (reference) and 29 degrees C. We show that high 
      temperature induced host physiological processes that are unfavorable to the 
      viral infection. Temperature influenced the expression of transcripts related to 
      the immune process and increased the transcription of genes related to the 
      apoptotic process, synaptic signaling and protein processes at 29 degrees C. 
      Concomitantly, the expression of genes associated with catabolism, metabolite 
      transport, macromolecule synthesis and cell growth remained low from the first 
      stage of infection at 29 degrees C. Moreover, viral entry into the host might have been 
      limited at 29 degrees C by changes in extracellular matrix composition and protein 
      abundance. Overall, these results provide new insights into how environmental 
      factors modulate host-pathogen interactions.
CI  - (c) 2020. Published by The Company of Biologists Ltd.
FAU - Delisle, Lizenn
AU  - Delisle L
AD  - Ifremer, Universite de Brest, CNRS, IRD, LEMAR, F-29280 Plouzane, France.
AD  - Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson 7042, New 
      Zealand.
FAU - Pauletto, Marianna
AU  - Pauletto M
AD  - Department of Comparative Biomedicine and Food Science, University of Padova, 
      Viale dell'Universita 16, 35020 Legnaro, Padova, Italy.
FAU - Vidal-Dupiol, Jeremie
AU  - Vidal-Dupiol J
AD  - IHPE, Universite de Montpellier, CNRS, Ifremer, Universite de Perpignan, Via 
      Domitia, F-34095 Montpellier, France.
FAU - Petton, Bruno
AU  - Petton B
AD  - Ifremer, Universite de Brest, CNRS, IRD, LEMAR, F-29280 Plouzane, France.
FAU - Bargelloni, Luca
AU  - Bargelloni L
AD  - Department of Comparative Biomedicine and Food Science, University of Padova, 
      Viale dell'Universita 16, 35020 Legnaro, Padova, Italy.
FAU - Montagnani, Caroline
AU  - Montagnani C
AD  - IHPE, Universite de Montpellier, CNRS, Ifremer, Universite de Perpignan, Via 
      Domitia, F-34095 Montpellier, France.
FAU - Pernet, Fabrice
AU  - Pernet F
AD  - Ifremer, Universite de Brest, CNRS, IRD, LEMAR, F-29280 Plouzane, France.
FAU - Corporeau, Charlotte
AU  - Corporeau C
AD  - Ifremer, Universite de Brest, CNRS, IRD, LEMAR, F-29280 Plouzane, France.
FAU - Fleury, Elodie
AU  - Fleury E
AUID- ORCID: 0000-0002-8546-6680
AD  - Ifremer, Universite de Brest, CNRS, IRD, LEMAR, F-29280 Plouzane, France 
      elodie.fleury@ifremer.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201015
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
SB  - IM
MH  - Animals
MH  - *Crassostrea/genetics
MH  - France
MH  - *Herpesviridae/genetics
MH  - Temperature
MH  - Transcriptome
PMC - PMC7578350
OTO - NOTNLM
OT  - Anti-viral molecular pathway
OT  - Host-pathogen interaction
OT  - Marine disease
OT  - OsHV-1
OT  - Resistance
OT  - Temperature
COIS- Competing interestsThe authors declare no competing or financial interests.
EDAT- 2020/08/21 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/10/15
CRDT- 2020/08/21 06:00
PHST- 2020/04/06 00:00 [received]
PHST- 2020/08/07 00:00 [accepted]
PHST- 2020/08/21 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/08/21 06:00 [entrez]
PHST- 2020/10/15 00:00 [pmc-release]
AID - jeb.226233 [pii]
AID - JEB226233 [pii]
AID - 10.1242/jeb.226233 [doi]
PST - epublish
SO  - J Exp Biol. 2020 Oct 15;223(Pt 20):jeb226233. doi: 10.1242/jeb.226233.