PMID- 32817921
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 2452-199X (Electronic)
IS  - 2452-199X (Linking)
VI  - 6
IP  - 1
DP  - 2021 Jan
TI  - Regulation of the inflammatory cycle by a controllable release hydrogel for 
      eliminating postoperative inflammation after discectomy.
PG  - 146-157
LID - 10.1016/j.bioactmat.2020.07.008 [doi]
AB  - Surgery is the final choice for most patients with intervertebral disc 
      degeneration (IDD). Operation-caused trauma will cause inflammation in the 
      intervertebral disc. Serious inflammation will cause tissue defects and induce 
      tissue degeneration, IDD recurrence and the occurrence of other diseases. 
      Therefore, we proposed a scheme to treat recurrence after discectomy by 
      inhibiting inflammation with an aspirin (ASP)-loaded hydrogel to restore the 
      mechanical stability of the spine and relieve local inflammation. ASP-liposomes 
      (ASP-Lips) were incorporated into a photocrosslinkable gelatin-methacryloyl 
      (GelMA) via mixing. This material can effectively alleviate inflammation by 
      inhibiting the release of high mobility group box 1 (HMGB1) from the nucleus to 
      the cytoplasm. We further assessed the expression of inflammatory cytokines, such 
      as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), and 
      degeneration-related factors, such as type II collagen (COL-2), Aggrecan, matrix 
      metallopeptidases-3 (MMP-3), MMP-13, a disintegrin and metalloproteinase with 
      thrombospondin motifs-4 (ADAMTS-4) and ADAMTS-5 in rat nucleus pulpous cells. The 
      level of IDD was analyzed through H&E, safranin-O staining and 
      immunohistochemistry in rabbit samples. In vitro, we found that ASP-Lip@GelMA 
      treatment significantly decreased inflammatory cytokines, MMP-3 and -13, and 
      ADAMTS-4 and -5 and up-regulated COL-2 and Aggrecan via the inhibited release of 
      HMGB-1 from the nucleus. In vivo, ASP-Lip@GelMA can effectively inhibit 
      inflammation of local tissue after disc surgery and fill local tissue defects. 
      This composite hydrogel system is a promising way to treat the recurrence of IDD 
      after surgery without persistent complications.
CI  - (c) 2020 [The Author/The Authors].
FAU - Liu, Yu
AU  - Liu Y
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Du, Jiacheng
AU  - Du J
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Peng, Peng
AU  - Peng P
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Cheng, Ruoyu
AU  - Cheng R
AD  - Shanghai Institute of Traumatology and Orthopaedics, Shanghai Key Laboratory for 
      Prevention and Treatment of Bone and Joint Diseases, Ruijin Hospital, Shanghai 
      Jiao Tong University School of Medicine, 197 Ruijin Second Road, Shanghai, 
      200025, PR China.
FAU - Lin, Jiayi
AU  - Lin J
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Xu, Congxin
AU  - Xu C
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Yang, Huilin
AU  - Yang H
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Cui, Wenguo
AU  - Cui W
AD  - Shanghai Institute of Traumatology and Orthopaedics, Shanghai Key Laboratory for 
      Prevention and Treatment of Bone and Joint Diseases, Ruijin Hospital, Shanghai 
      Jiao Tong University School of Medicine, 197 Ruijin Second Road, Shanghai, 
      200025, PR China.
FAU - Mao, Haiqing
AU  - Mao H
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
FAU - Li, Yuling
AU  - Li Y
AD  - Shanghai Institute of Traumatology and Orthopaedics, Shanghai Key Laboratory for 
      Prevention and Treatment of Bone and Joint Diseases, Ruijin Hospital, Shanghai 
      Jiao Tong University School of Medicine, 197 Ruijin Second Road, Shanghai, 
      200025, PR China.
AD  - Department of Orthopaedics, Affiliated Hospital of North Sichuan Medical College, 
      No.63 Wenhua Road, Nanchong, Sichuan, 637000, PR China.
FAU - Geng, Dechun
AU  - Geng D
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, 215006, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200812
PL  - China
TA  - Bioact Mater
JT  - Bioactive materials
JID - 101685294
PMC - PMC7426539
OTO - NOTNLM
OT  - Aspirin
OT  - Composite hydrogel
OT  - Inflammation
OT  - Intervertebral disc degeneration
OT  - Recurrence
COIS- The authors declare no conflict of interest.
EDAT- 2020/08/21 06:00
MHDA- 2020/08/21 06:01
PMCR- 2020/08/12
CRDT- 2020/08/21 06:00
PHST- 2020/04/06 00:00 [received]
PHST- 2020/07/03 00:00 [revised]
PHST- 2020/07/17 00:00 [accepted]
PHST- 2020/08/21 06:00 [entrez]
PHST- 2020/08/21 06:00 [pubmed]
PHST- 2020/08/21 06:01 [medline]
PHST- 2020/08/12 00:00 [pmc-release]
AID - S2452-199X(20)30134-1 [pii]
AID - 10.1016/j.bioactmat.2020.07.008 [doi]
PST - epublish
SO  - Bioact Mater. 2020 Aug 12;6(1):146-157. doi: 10.1016/j.bioactmat.2020.07.008. 
      eCollection 2021 Jan.