PMID- 32820304
OWN - NLM
STAT- MEDLINE
DCOM- 20200924
LR  - 20220531
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 72
IP  - 4
DP  - 2020 Aug 25
TI  - [FOXO4 maintains senescence in human umbilical cord mesenchymal stem cells by 
      repressing apoptosis].
PG  - 426-432
AB  - The purpose of the present study was to investigate the effects of forkhead box 
      O4 (FOXO4) on the senescence of human umbilical cord-derived mesenchymal stem 
      cells (hUC-MSCs). The hUC-MSCs were induced to senescence by natural passage, and 
      FOXO4 expression was inhibited by lentiviral shRNA transfection. The hallmark of 
      cell senescence was analyzed by beta-galactosidase staining, and the cell viability 
      was assayed by CCK-8 method. Flow cytometry was used to investigate the apoptosis 
      of hUC-MSCs. The expression levels of Bcl-2, Bax, FOXO4, interleukin 6 (IL-6) and 
      cleaved Caspase-3 were detected by qPCR and Western blot. Immunofluorescence 
      staining was used to detect FOXO4 expression. The amount of IL-6 secreted by 
      hUC-MSCs was detected by ELISA. The results showed that, compared with the 
      passage 1, senescent hUC-MSCs showed up-regulated expression levels of Bax and 
      FOXO4, down-regulated expression levels of Bcl-2 and cleaved Caspase-3, and 
      increased IL-6 mRNA expression and secretion. FOXO4 inhibition in senescent 
      hUC-MSCs promoted cell apoptosis, reduced cell viability, and inhibited the mRNA 
      expression and secretion of IL-6. These results suggest that FOXO4 maintains 
      viability and function of senescent hUC-MSCs by repressing their apoptosis 
      response, thus accelerating senescence of the whole cell colony.
FAU - Wu, Ping-Ping
AU  - Wu PP
AD  - Department of Orthopaedics, Jiujiang University Affiliated Hospital, Jiujiang 
      332000, China.
FAU - Hu, Wen-Long
AU  - Hu WL
AD  - Department of Orthopaedics, Jiujiang University Affiliated Hospital, Jiujiang 
      332000, China. huwenlong1988@126.com.
FAU - Yin, Chang-Chang
AU  - Yin CC
AD  - Department of Biochemistry, College of Basic Medicine, Jiujiang University, 
      Jiujiang 332000, China.
FAU - Fei, Jiu-Wang
AU  - Fei JW
AD  - Department of Biochemistry, College of Basic Medicine, Jiujiang University, 
      Jiujiang 332000, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (FOXO4 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Apoptosis
MH  - Cell Cycle Proteins
MH  - Cell Survival
MH  - Cellular Senescence
MH  - Forkhead Transcription Factors
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Transcription Factors
MH  - Umbilical Cord
EDAT- 2020/08/21 06:00
MHDA- 2020/09/25 06:00
CRDT- 2020/08/22 06:00
PHST- 2020/08/22 06:00 [entrez]
PHST- 2020/08/21 06:00 [pubmed]
PHST- 2020/09/25 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2020 Aug 25;72(4):426-432.