PMID- 32820455
OWN - NLM
STAT- MEDLINE
DCOM- 20211123
LR  - 20240226
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 28
IP  - 1
DP  - 2021 Jan
TI  - Estrogen Regulates Local Cysteine Metabolism in Mouse Myometrium.
PG  - 79-90
LID - 10.1007/s43032-020-00284-6 [doi]
AB  - Sulfur amino acid metabolism influences reproductive physiology, and 
      transsulfuration in particular may be critical for normal cellular function. The 
      sex hormone estrogen (E(2)) modulates gene expression and redox balance in some 
      tissues by inducing the transsulfuration enzymes cystathionine beta-synthase (CBS) 
      and cystathionine gamma-lyase (CSE). The role of sex hormones in sulfur amino acid 
      metabolism by uterine smooth muscle is not known. Here, we show that CBS and CSE 
      proteins increase in the mouse myometrium during estrus and diestrus, 
      respectively, suggesting that E(2) reciprocally regulates myometrial CBS and CSE 
      expression. In ovariectomized mice, exogenous E(2) upregulates CBS and 
      downregulates CSE levels. E(2) promotes CBS mRNA and protein expression but 
      attenuates CSE protein expression without affecting CSE mRNA. This pattern of 
      E(2)-stimulated changes in transsulfuration enzyme expression is specific to the 
      uterine smooth muscle. E(2) does not change vaginal or cervical expression of CBS 
      or CSE significantly, and E(2) decreases expression of CSE in the liver without 
      affecting CBS. E(2) also downregulates myometrial cysteinesulfinic acid 
      decarboxylase (CSAD) and decreases myometrial biochemical synthesis of the 
      gaso-transmitter hydrogen sulfide (H(2)S). These findings suggest that myometrial 
      sulfur amino acid metabolism may regulate uterine redox homeostasis, with 
      implications for the source and metabolism of myometrial cysteine in high E(2) 
      states such as estrus and pregnancy.
FAU - Guerra, Damian D
AU  - Guerra DD
AD  - Department of Biology , University of Louisville , 2301 South 3rd Street, 
      Louisville, Kentucky, 40292, USA.
FAU - Bok, Rachael
AU  - Bok R
AD  - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, 
      University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, 
      CO, 80045, USA.
FAU - Breen, Kelsey
AU  - Breen K
AD  - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, 
      University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, 
      CO, 80045, USA.
FAU - Vyas, Vibhuti
AU  - Vyas V
AD  - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, 
      University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, 
      CO, 80045, USA.
FAU - Jiang, Hua
AU  - Jiang H
AD  - Section of Clinical Genetics and Metabolism, Department of Pediatrics, University 
      of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, 
      USA.
FAU - MacLean, Kenneth N
AU  - MacLean KN
AD  - Section of Clinical Genetics and Metabolism, Department of Pediatrics, University 
      of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, 
      USA.
FAU - Hurt, K Joseph
AU  - Hurt KJ
AUID- ORCID: 0000-0001-6906-350X
AD  - Division of Reproductive Sciences, Department of Obstetrics and Gynecology, 
      University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, 
      CO, 80045, USA. k.joseph.hurt@cuanschutz.edu.
AD  - Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, 
      University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Mail Stop 
      8613, Aurora, CO, 80045, USA. k.joseph.hurt@cuanschutz.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200820
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 1EQV5MLY3D (Taurine)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 4.2.1.22 (Cystathionine beta-Synthase)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cystathionine beta-Synthase/genetics/metabolism
MH  - Cystathionine gamma-Lyase/genetics/metabolism
MH  - Cysteine/*metabolism
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Humans
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myocytes, Smooth Muscle/*drug effects/metabolism
MH  - Myometrium/*drug effects/metabolism
MH  - Ovariectomy
MH  - Progesterone/pharmacology
MH  - Taurine/metabolism
MH  - Mice
OTO - NOTNLM
OT  - Biochemistry
OT  - Cystathionine
OT  - Gaso-transmitter
OT  - Nitric oxide
OT  - One-carbon metabolism
OT  - Taurine
OT  - Transsulfuration
OT  - Uterine smooth muscle
EDAT- 2020/08/21 06:00
MHDA- 2021/11/24 06:00
CRDT- 2020/08/22 06:00
PHST- 2020/05/18 00:00 [received]
PHST- 2020/08/02 00:00 [accepted]
PHST- 2020/08/21 06:00 [pubmed]
PHST- 2021/11/24 06:00 [medline]
PHST- 2020/08/22 06:00 [entrez]
AID - 10.1007/s43032-020-00284-6 [pii]
AID - 10.1007/s43032-020-00284-6 [doi]
PST - ppublish
SO  - Reprod Sci. 2021 Jan;28(1):79-90. doi: 10.1007/s43032-020-00284-6. Epub 2020 Aug 
      20.