PMID- 32821104
OWN - NLM
STAT- MEDLINE
DCOM- 20200903
LR  - 20240329
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 15
DP  - 2020
TI  - 3D-HA Scaffold Functionalized by Extracellular Matrix of Stem Cells Promotes Bone 
      Repair.
PG  - 5825-5838
LID - 10.2147/IJN.S259678 [doi]
AB  - BACKGROUND AND PURPOSE: The extracellular matrix (ECM) derived from bone marrow 
      mesenchymal stem cells (BMSCs) has been used in regenerative medicine because of 
      its good biological activity; however, its poor mechanical properties limit its 
      application in bone regeneration. The purpose of this study is to construct a 
      three dimensional-printed hydroxyapatite (3D-HA)/BMSC-ECM composite scaffold that 
      not only has biological activity but also sufficient mechanical strength and 
      reasonably distributed spatial structure. METHODS: A BMSC-ECM was first extracted 
      and formed into micron-sized particles, and then the ECM particles were modified 
      onto the surface of 3D-HA scaffolds using an innovative linking method to 
      generate composite 3D-HA/BMSC-ECM scaffolds. The 3D-HA scaffolds were used as the 
      control group. The basic properties, biocompatibility and osteogenesis ability of 
      both scaffolds were tested in vitro. Finally, a critical skull defect rat model 
      was created and the osteogenesis effect of the scaffolds was evaluated in vivo. 
      RESULTS: The compressive modulus of the composite scaffolds reached 9.45+/-0.32 
      MPa, which was similar to that of the 3D-HA scaffolds (p>0.05). The pore size of 
      the two scaffolds was 305+/-47 um and 315+/-34 um (p>0.05), respectively. A CCK-8 
      assay indicated that the scaffolds did not have cytotoxicity. The composite 
      scaffolds had good cell adhesion ability, with a cell adhesion rate of up to 
      76.00+/-6.17% after culturing for 7 hours, while that of the 3D-HA scaffolds was 
      51.85+/-4.77% (p<0.01). In addition, the composite scaffold displayed higher 
      alkaline phosphatase (ALP) activity, osteogenesis-related mRNA expression, and 
      calcium nodule formation, thus confirming that the composite scaffolds had good 
      osteogenic activity. The composite scaffolds exhibited good bone repair in vivo 
      and were superior to the 3D-HA scaffolds. CONCLUSION: We conclude that BMSC-ECM 
      is a good osteogenic material and that the composite scaffolds have good 
      osteogenic ability, which provides a new method and concept for the repair of 
      bone defects.
CI  - (c) 2020 Chi et al.
FAU - Chi, Hui
AU  - Chi H
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - Chen, Guanghua
AU  - Chen G
AUID- ORCID: 0000-0003-1388-5584
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - He, Yixin
AU  - He Y
AD  - Department of Nephrology, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - Chen, Guanghao
AU  - Chen G
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - Tu, Hualei
AU  - Tu H
AD  - Department of Burn, The Fifth Hospital of Harbin Medical University, Harbin, 
      People's Republic of China.
FAU - Liu, Xiaoqi
AU  - Liu X
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - Yan, Jinglong
AU  - Yan J
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - Wang, Xiaoyan
AU  - Wang X
AUID- ORCID: 0000-0002-3299-9117
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20200806
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (RNA, Messenger)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - Bone Regeneration/drug effects
MH  - Bone and Bones/diagnostic imaging/drug effects/pathology
MH  - Cell Adhesion/drug effects
MH  - Cell Death/drug effects
MH  - Cell Differentiation/drug effects
MH  - Durapatite/*pharmacology
MH  - Extracellular Matrix/drug effects/*metabolism
MH  - Hydrodynamics
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/drug effects/ultrastructure
MH  - Osteogenesis/drug effects/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Tissue Scaffolds/*chemistry
MH  - Wound Healing/drug effects
PMC - PMC7418460
OTO - NOTNLM
OT  - 3D printed scaffold
OT  - bone tissue engineering
OT  - extracellular matrix
OT  - osteogenesis
OT  - stem cells
COIS- The authors report no conflicts of interest for this work.
EDAT- 2020/08/22 06:00
MHDA- 2020/09/04 06:00
PMCR- 2020/08/06
CRDT- 2020/08/22 06:00
PHST- 2020/04/29 00:00 [received]
PHST- 2020/07/13 00:00 [accepted]
PHST- 2020/08/22 06:00 [entrez]
PHST- 2020/08/22 06:00 [pubmed]
PHST- 2020/09/04 06:00 [medline]
PHST- 2020/08/06 00:00 [pmc-release]
AID - 259678 [pii]
AID - 10.2147/IJN.S259678 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2020 Aug 6;15:5825-5838. doi: 10.2147/IJN.S259678. 
      eCollection 2020.