PMID- 32821821
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20230829
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 6
IP  - 32
DP  - 2020 Aug
TI  - Life span extension by glucose restriction is abrogated by methionine 
      supplementation: Cross-talk between glucose and methionine and implication of 
      methionine as a key regulator of life span.
PG  - eaba1306
LID - 10.1126/sciadv.aba1306 [doi]
LID - eaba1306
AB  - Caloric restriction (CR) is known to extend life span across species; however, 
      the molecular mechanisms are not well understood. We investigate the mechanism by 
      which glucose restriction (GR) extends yeast replicative life span, by combining 
      ribosome profiling and RNA-seq with microfluidic-based single-cell analysis. We 
      discovered a cross-talk between glucose sensing and the regulation of 
      intracellular methionine: GR down-regulated the transcription and translation of 
      methionine biosynthetic enzymes and transporters, leading to a decreased 
      intracellular methionine concentration; external supplementation of methionine 
      cancels the life span extension by GR. Furthermore, genetic perturbations that 
      decrease methionine synthesis/uptake extend life span. These observations suggest 
      that intracellular methionine mediates the life span effects of various nutrient 
      and genetic perturbations, and that the glucose-methionine cross-talk is a 
      general mechanism for coordinating the nutrient status and the translation/growth 
      of a cell. Our work also implicates proteasome as a downstream effector of the 
      life span extension by GR.
CI  - Copyright (c) 2020 The Authors, some rights reserved; exclusive licensee American 
      Association for the Advancement of Science. No claim to original U.S. Government 
      Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 
      (CC BY-NC).
FAU - Zou, Ke
AU  - Zou K
AUID- ORCID: 0000-0003-4860-4439
AD  - Department of Biochemistry and Biophysics, University of California, San 
      Francisco, San Francisco, CA 94158, USA.
AD  - The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, 
      School of Physics, Peking University, Beijing, China.
AD  - Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, 
      Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 
      100871, China.
FAU - Rouskin, Silvia
AU  - Rouskin S
AUID- ORCID: 0000-0003-2042-6642
AD  - Department of Cellular and Molecular Pharmacology, University of California, San 
      Francisco, California Institute for Quantitative Biomedical Research and Howard 
      Hughes Medical Institute, San Francisco, CA 94158, USA.
FAU - Dervishi, Kevin
AU  - Dervishi K
AD  - Harvard Medical School, Boston, MA 02115, USA.
FAU - McCormick, Mark A
AU  - McCormick MA
AD  - Department of Biochemistry and Molecular Biology, School of Medicine, University 
      of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.
AD  - Autophagy Inflammation and Metabolism Center of Biomedical Research Excellence, 
      Albuquerque, NM 87131, USA.
FAU - Sasikumar, Arjun
AU  - Sasikumar A
AD  - Buck Institute for Research on Aging, Novato, CA 94945, USA.
FAU - Deng, Changhui
AU  - Deng C
AD  - Department of Biochemistry and Biophysics, University of California, San 
      Francisco, San Francisco, CA 94158, USA.
FAU - Chen, Zhibing
AU  - Chen Z
AUID- ORCID: 0000-0003-4860-4439
AD  - Department of Physical Therapy and Rehabilitation Science, University of 
      California, San Francisco, San Francisco, CA 94158, USA.
FAU - Kaeberlein, Matt
AU  - Kaeberlein M
AUID- ORCID: 0000-0002-1311-3421
AD  - Department of Pathology, University of Washington, Seattle, WA 98195, USA.
FAU - Brem, Rachel B
AU  - Brem RB
AUID- ORCID: 0000-0002-8587-9434
AD  - Buck Institute for Research on Aging, Novato, CA 94945, USA.
FAU - Polymenis, Michael
AU  - Polymenis M
AUID- ORCID: 0000-0003-1507-0936
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      TX 77843, USA.
FAU - Kennedy, Brian K
AU  - Kennedy BK
AUID- ORCID: 0000-0002-5754-1874
AD  - Buck Institute for Research on Aging, Novato, CA 94945, USA.
AD  - Departments of Biochemistry and Physiology, Yong Loo Lin School of Medicine, 
      National University of Singapore, Singapore.
AD  - Centre for Healthy Longevity, National University Health System, Singapore.
AD  - Singapore Institute for Clinical Sciences, ASTAR, Singapore.
FAU - Weissman, Jonathan S
AU  - Weissman JS
AUID- ORCID: 0000-0003-2445-670X
AD  - Department of Cellular and Molecular Pharmacology, University of California, San 
      Francisco, California Institute for Quantitative Biomedical Research and Howard 
      Hughes Medical Institute, San Francisco, CA 94158, USA.
FAU - Zheng, Jiashun
AU  - Zheng J
AUID- ORCID: 0000-0003-3266-4461
AD  - Department of Biochemistry and Biophysics, University of California, San 
      Francisco, San Francisco, CA 94158, USA.
FAU - Ouyang, Qi
AU  - Ouyang Q
AUID- ORCID: 0000-0003-1585-7368
AD  - The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, 
      School of Physics, Peking University, Beijing, China.
AD  - Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, 
      Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 
      100871, China.
FAU - Li, Hao
AU  - Li H
AUID- ORCID: 0000-0003-4889-953X
AD  - Department of Biochemistry and Biophysics, University of California, San 
      Francisco, San Francisco, CA 94158, USA.
LA  - eng
GR  - R01 GM123139/GM/NIGMS NIH HHS/United States
GR  - R21 AG060129/AG/NIA NIH HHS/United States
GR  - P01 AG001751/AG/NIA NIH HHS/United States
GR  - P20 GM121176/GM/NIGMS NIH HHS/United States
GR  - R01 AG043080/AG/NIA NIH HHS/United States
GR  - P30 AG013280/AG/NIA NIH HHS/United States
GR  - R01 AG058742/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20200805
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - AE28F7PNPL (Methionine)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Dietary Supplements
MH  - Glucose/pharmacology
MH  - *Longevity
MH  - *Methionine
MH  - Saccharomyces cerevisiae/genetics
PMC - PMC7406366
EDAT- 2020/08/22 06:00
MHDA- 2022/04/12 06:00
PMCR- 2020/08/05
CRDT- 2020/08/22 06:00
PHST- 2019/11/07 00:00 [received]
PHST- 2020/06/22 00:00 [accepted]
PHST- 2020/08/22 06:00 [entrez]
PHST- 2020/08/22 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2020/08/05 00:00 [pmc-release]
AID - aba1306 [pii]
AID - 10.1126/sciadv.aba1306 [doi]
PST - epublish
SO  - Sci Adv. 2020 Aug 5;6(32):eaba1306. doi: 10.1126/sciadv.aba1306. eCollection 2020 
      Aug.