PMID- 32823052
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231103
IS  - 2589-0042 (Electronic)
IS  - 2589-0042 (Linking)
VI  - 23
IP  - 9
DP  - 2020 Sep 25
TI  - Adsorption Sequencing as a Rapid Method to Link Environmental Bacteriophages to 
      Hosts.
PG  - 101439
LID - 10.1016/j.isci.2020.101439 [doi]
LID - 101439
AB  - An important viromics challenge is associating bacteriophages to hosts. To 
      address this, we developed adsorption sequencing (AdsorpSeq), a readily 
      implementable method to measure phages that are preferentially adsorbed to 
      specific host cell envelopes. AdsorpSeq thus captures the key initial infection 
      cycle step. Phages are added to cell envelopes, adsorbed phages are isolated 
      through gel electrophoresis, after which adsorbed phage DNA is sequenced and 
      compared with the full virome. Here, we show that AdsorpSeq allows for separation 
      of phages based on receptor-adsorbing capabilities. Next, we applied AdsorpSeq to 
      identify phages in a wastewater virome that adsorb to cell envelopes of nine 
      bacteria, including important pathogens. We detected 26 adsorbed phages including 
      common and rare members of the virome, a minority being related to previously 
      characterized phages. We conclude that AdsorpSeq is an effective new tool for 
      rapid characterization of environmental phage adsorption, with a 
      proof-of-principle application to Gram-negative host cell envelopes.
CI  - (c) 2020 The Authors.
FAU - de Jonge, Patrick A
AU  - de Jonge PA
AD  - Theoretical Biology and Bioinformatics, Science4Life, Utrecht University, 3584 CH 
      Utrecht, the Netherlands.
AD  - Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of 
      Technology, 2629 HZ Delft, the Netherlands.
FAU - von Meijenfeldt, F A Bastiaan
AU  - von Meijenfeldt FAB
AD  - Theoretical Biology and Bioinformatics, Science4Life, Utrecht University, 3584 CH 
      Utrecht, the Netherlands.
FAU - Costa, Ana Rita
AU  - Costa AR
AD  - Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of 
      Technology, 2629 HZ Delft, the Netherlands.
FAU - Nobrega, Franklin L
AU  - Nobrega FL
AD  - Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of 
      Technology, 2629 HZ Delft, the Netherlands.
FAU - Brouns, Stan J J
AU  - Brouns SJJ
AD  - Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of 
      Technology, 2629 HZ Delft, the Netherlands.
FAU - Dutilh, Bas E
AU  - Dutilh BE
AD  - Theoretical Biology and Bioinformatics, Science4Life, Utrecht University, 3584 CH 
      Utrecht, the Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20200806
PL  - United States
TA  - iScience
JT  - iScience
JID - 101724038
PMC - PMC7452251
OTO - NOTNLM
OT  - Ecology
OT  - Environmental Science
OT  - Microbiology
OT  - Techniques in Genetics
COIS- The authors declare no competing interests.
EDAT- 2020/08/22 06:00
MHDA- 2020/08/22 06:01
PMCR- 2020/08/06
CRDT- 2020/08/22 06:00
PHST- 2020/05/11 00:00 [received]
PHST- 2020/07/23 00:00 [revised]
PHST- 2020/08/03 00:00 [accepted]
PHST- 2020/08/22 06:01 [medline]
PHST- 2020/08/22 06:00 [pubmed]
PHST- 2020/08/22 06:00 [entrez]
PHST- 2020/08/06 00:00 [pmc-release]
AID - S2589-0042(20)30631-3 [pii]
AID - 101439 [pii]
AID - 10.1016/j.isci.2020.101439 [doi]
PST - epublish
SO  - iScience. 2020 Aug 6;23(9):101439. doi: 10.1016/j.isci.2020.101439. eCollection 
      2020 Sep 25.