PMID- 32825252
OWN - NLM
STAT- MEDLINE
DCOM- 20210413
LR  - 20210413
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Aug 20
TI  - Transcriptional Changes Involved in Atrophying Muscles during Prolonged Fasting 
      in Rats.
LID - 10.3390/ijms21175984 [doi]
LID - 5984
AB  - Food deprivation resulting in muscle atrophy may be detrimental to health. To 
      better understand how muscle mass is regulated during such a nutritional 
      challenge, the current study deciphered muscle responses during phase 2 (P2, 
      protein sparing) and phase 3 (P3, protein mobilization) of prolonged fasting in 
      rats. This was done using transcriptomics analysis and a series of biochemistry 
      measurements. The main findings highlight changes for plasma catabolic and 
      anabolic stimuli, as well as for muscle transcriptome, energy metabolism, and 
      oxidative stress. Changes were generally consistent with the intense use of 
      lipids as fuels during P2. They also reflected increased muscle protein 
      degradation and repressed synthesis, in a more marked manner during P3 than P2 
      compared to the fed state. Nevertheless, several unexpected changes appeared to 
      be in favor of muscle protein synthesis during fasting, notably at the level of 
      the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target 
      of rapamycin (mTOR) signaling pathway, transcription and translation processes, 
      and the response to oxidative stress. Such mechanisms might promote protein 
      sparing during P2 and prepare the restoration of the protein compartment during 
      P3 in anticipation of food intake for optimizing the effects of an upcoming 
      refeeding, thereby promoting body maintenance and survival. Future studies should 
      examine relevance of such targets for improving nitrogen balance during catabolic 
      diseases.
FAU - Ibrahim, Marianne
AU  - Ibrahim M
AUID- ORCID: 0000-0002-6122-7167
AD  - Institut Pluridisciplinaire Hubert Curien (IPHC), CNRS, Universite de Strasbourg, 
      67000 Strasbourg, France.
AD  - Laboratoire de Spectrometrie de Masse Bio-Organique, 25 rue Becquerel, F-67087 
      Strasbourg, France.
FAU - Wasselin, Thierry
AU  - Wasselin T
AD  - Institut Pluridisciplinaire Hubert Curien (IPHC), CNRS, Universite de Strasbourg, 
      67000 Strasbourg, France.
AD  - Laboratoire de Spectrometrie de Masse Bio-Organique, 25 rue Becquerel, F-67087 
      Strasbourg, France.
FAU - Challet, Etienne
AU  - Challet E
AUID- ORCID: 0000-0001-9416-9496
AD  - Institute of Cellular and Integrative Neurosciences, CNRS, Universite de 
      Strasbourg, F-67000 Strasbourg, France.
FAU - Van Dorsselaer, Alain
AU  - Van Dorsselaer A
AD  - Institut Pluridisciplinaire Hubert Curien (IPHC), CNRS, Universite de Strasbourg, 
      67000 Strasbourg, France.
AD  - Laboratoire de Spectrometrie de Masse Bio-Organique, 25 rue Becquerel, F-67087 
      Strasbourg, France.
FAU - Le Maho, Yvon
AU  - Le Maho Y
AD  - Institut Pluridisciplinaire Hubert Curien (IPHC), CNRS, Universite de Strasbourg, 
      67000 Strasbourg, France.
AD  - Departement Ecologie, Physiologie, Ethologie, 23 rue Becquerel, F-67087 
      Strasbourg, France.
AD  - Centre Scientifique de Monaco, 8 quai Antoine 1er, 98000 Monaco, Monaco.
FAU - Raclot, Thierry
AU  - Raclot T
AD  - Institut Pluridisciplinaire Hubert Curien (IPHC), CNRS, Universite de Strasbourg, 
      67000 Strasbourg, France.
AD  - Departement Ecologie, Physiologie, Ethologie, 23 rue Becquerel, F-67087 
      Strasbourg, France.
FAU - Bertile, Fabrice
AU  - Bertile F
AUID- ORCID: 0000-0001-5510-4868
AD  - Institut Pluridisciplinaire Hubert Curien (IPHC), CNRS, Universite de Strasbourg, 
      67000 Strasbourg, France.
AD  - Laboratoire de Spectrometrie de Masse Bio-Organique, 25 rue Becquerel, F-67087 
      Strasbourg, France.
LA  - eng
GR  - ANR-05-BLAN-0069/Agence Nationale de la Recherche/
GR  - ANR-10-INSB-08-03/Proteomics French Infrastructure (ProFI)/
PT  - Journal Article
DEP - 20200820
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Hormones)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Muscle Proteins)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - Animals
MH  - Energy Metabolism/genetics
MH  - Fasting/*physiology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Hormones/blood
MH  - Intercellular Signaling Peptides and Proteins/blood
MH  - Male
MH  - Muscle Proteins/*genetics/metabolism
MH  - Muscle, Skeletal/metabolism/physiopathology
MH  - Muscular Atrophy/*genetics/metabolism
MH  - Oxidative Stress/*genetics/physiology
MH  - Rats, Sprague-Dawley
MH  - Urea/blood
PMC - PMC7503389
OTO - NOTNLM
OT  - fasting
OT  - fuel metabolism
OT  - muscle
OT  - oxidative stress
OT  - protein breakdown
OT  - protein synthesis
OT  - rat
OT  - transcriptomics
COIS- The authors declare no conflict of interest.
EDAT- 2020/08/23 06:00
MHDA- 2021/04/14 06:00
PMCR- 2020/09/01
CRDT- 2020/08/23 06:00
PHST- 2020/07/03 00:00 [received]
PHST- 2020/08/18 00:00 [revised]
PHST- 2020/08/18 00:00 [accepted]
PHST- 2020/08/23 06:00 [entrez]
PHST- 2020/08/23 06:00 [pubmed]
PHST- 2021/04/14 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21175984 [pii]
AID - ijms-21-05984 [pii]
AID - 10.3390/ijms21175984 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Aug 20;21(17):5984. doi: 10.3390/ijms21175984.