PMID- 32825967
OWN - NLM
STAT- MEDLINE
DCOM- 20210701
LR  - 20210701
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 216
IP  - 9
DP  - 2020 Sep
TI  - Circ_0000218 plays a carcinogenic role in laryngeal cancer through regulating 
      microRNA-139-3p/Smad3 axis.
PG  - 153103
LID - S0344-0338(20)31958-0 [pii]
LID - 10.1016/j.prp.2020.153103 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) accounts for about 85%-90% 
      of all cases of laryngeal cancer. So far, the role and molecular mechanism of 
      circular RNA 0,000,218 (circ_0000218)/microRNA (miR)-139-3p in laryngeal cancer 
      are not clear. The present study aimed to investigate the role and regulatory 
      mechanism of circ_0000218/miR-139-3p in laryngeal cancerin vitro and in vivo. 
      METHODS: quantitative real time polymerase chain reaction (qRT-PCR) was used to 
      detect the expression of circ_0000218/miR-139-3p in LSCC cells. Dual luciferase 
      reporter assay and RNA immunoprecipitation (RIP) assay were used to confirm 
      binding sites between miR-139-3p and smad family member 3 (Smad3), and 
      circ_0000218 and miR-139-3p. Cell Counting Kit-8 (CCK-8) and cell apoptosis 
      analysis were used to detect cell viability and apoptosis. Xenograft experiment 
      was performed to show in vivo effect of circ_0000218/miR-139-3p on the growth of 
      LSCC. RESULTS: Circ_0000218 was highly expressed in LSCC cells. miR-139-3p, lower 
      expressed in LSCC cells, was negatively regulated by circ_0000218 in LSCC cells. 
      Besides, the findings suggested that circ_0000218 silencing inhibited the LSCC 
      cell viability and promoted apoptosis by negatively regulating miR-139-3p 
      expression. Furthermore, the data indicated that miR-139-3p inhibited the 
      viability of LSCC cells and promoted apoptosis, and these effects were reversed 
      by Smad3 over-expression. In addition, the in vivo effects of 
      circ_0000218/miR-139-3p on LSCC were consistent with the in vitro study. 
      CONCLUSIONS: circ_0000218 inhibition inhibited the growth of LSCC by targeting 
      miR-139-3p/Smad3 axis. Our present study provided a new target for laryngeal 
      cancer treatment.
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Bai, Yiyang
AU  - Bai Y
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Hou, Jia
AU  - Hou J
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Wang, Xiao
AU  - Wang X
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Geng, Luying
AU  - Geng L
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Jia, Xiaohui
AU  - Jia X
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Xiang, Luochengling
AU  - Xiang L
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Nan, Kejun
AU  - Nan K
AD  - Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710061, China; Oncology Hospital, Xi'an International 
      Medical Center Hospital, Xi'an, Shaanxi 710075, China. Electronic address: 
      nankj0516@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200710
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Carcinogens)
RN  - 0 (MIRN139 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (Smad3 Protein)
SB  - IM
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Carcinogenesis/genetics
MH  - Carcinogens/metabolism
MH  - Carcinoma, Squamous Cell/pathology
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - MicroRNAs/*genetics
MH  - RNA, Circular/*genetics
MH  - Smad3 Protein/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/pathology
OTO - NOTNLM
OT  - Laryngeal cancer
OT  - Smad3
OT  - circ_0000218
OT  - microRNA-139-3p
EDAT- 2020/08/23 06:00
MHDA- 2021/07/02 06:00
CRDT- 2020/08/23 06:00
PHST- 2020/05/18 00:00 [received]
PHST- 2020/06/30 00:00 [revised]
PHST- 2020/07/07 00:00 [accepted]
PHST- 2020/08/23 06:00 [entrez]
PHST- 2020/08/23 06:00 [pubmed]
PHST- 2021/07/02 06:00 [medline]
AID - S0344-0338(20)31958-0 [pii]
AID - 10.1016/j.prp.2020.153103 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2020 Sep;216(9):153103. doi: 10.1016/j.prp.2020.153103. Epub 
      2020 Jul 10.