PMID- 32826238
OWN - NLM
STAT- MEDLINE
DCOM- 20210528
LR  - 20210528
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 50
IP  - 4
DP  - 2020 Jul
TI  - Correlation Research of Thymidine Synthase Expression in Circulating Tumor Cell 
      and Clinical Characteristics of Colon Cancer.
PG  - 439-446
AB  - OBJECTIVE: To explore the clinical significance of thymidine synthase (TYMS) 
      expression in circulating tumor cells (CTC). METHODS: Patients (n=43) were 
      recruited upon reference to the Department of Surgery at a local hospital. 
      Arterial-portal blood samples were harvested from all patients. Patient baseline 
      information was collected when individuals were recruited into the study and 
      include age, sex, and tumor stage. Complete response (CR) events and progressive 
      disease (PD) events were recorded after follow-up. The CTC positive rate and the 
      CTC number were assessed in blood samples. Epithelial-mesenchymal transition 
      (EMT) subgroups and related TYMS expressions were examined for their correlation 
      with colon cancer prognosis. The TYMS expression differed among various EMT 
      subgroups. RESULTS: In our study, the CTC number was not associated with the 
      prognosis index of colon cancer patients. These non-associated indices also 
      include TNM tumor stage, CEA factor, primary tumor position, pathological 
      pattern, age, and sex. However, the total CTC positive rate was correlated with 
      tumor stage. For patients with right colon cancer (10/35), mixed type EMT was in 
      the majority, while epithelial type EMT was the main subgroup in patients with 
      left colon cancer (25/35). The TYMS expression differed among various subgroups 
      of EMT. Left colon cancer (25/35) had the negative expression level of TYMS 
      (75%). CONCLUSIONS: CTC positive rate is a promising index for the diagnosis of 
      colon cancer. The lack of TYMS in CTC makes EMT cells prone to becoming 
      epithelial-like cells, and TYMS silence in CTC indicates that the tumor is in the 
      left colon.
CI  - (c) 2020 by the Association of Clinical Scientists, Inc.
FAU - Jiang, Yimei
AU  - Jiang Y
AD  - Departmant of General Surgery, Ruijin Hospital North, Shanghai Jiao Tong 
      University School of Medcine, Shanghai, China.
FAU - Liu, Kun
AU  - Liu K
AD  - Departmant of General Surgery, Ruijin Hospital North, Shanghai Jiao Tong 
      University School of Medcine, Shanghai, China.
FAU - Shi, Yiqin
AU  - Shi Y
AD  - Departmant of General Surgery, Ruijin Hospital North, Shanghai Jiao Tong 
      University School of Medcine, Shanghai, China.
FAU - Li, You
AU  - Li Y
AD  - Departmant of General Surgery, Ruijin Hospital North, Shanghai Jiao Tong 
      University School of Medcine, Shanghai, China.
FAU - Ji, Xiaopin
AU  - Ji X
AD  - Departmant of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medcine, Shanghai, China ren_zr@aliyun.com xp_ji18@aliyun.com.
FAU - Zhao, Ren
AU  - Zhao R
AD  - Departmant of General Surgery, Ruijin Hospital North, Shanghai Jiao Tong 
      University School of Medcine, Shanghai, China ren_zr@aliyun.com 
      xp_ji18@aliyun.com.
AD  - Departmant of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medcine, Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.1.1.45 (Thymidylate Synthase)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - China
MH  - Colonic Neoplasms/*metabolism
MH  - Epithelial Cells/metabolism
MH  - Epithelial-Mesenchymal Transition/physiology
MH  - Female
MH  - Gene Expression/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Cells, Circulating/metabolism/*pathology
MH  - Prognosis
MH  - Thymidine/metabolism
MH  - Thymidylate Synthase/*genetics/metabolism
MH  - Transcriptome/genetics
OTO - NOTNLM
OT  - Circulating tumor cell
OT  - Colon cancer
OT  - Prognosis
OT  - Thymidine synthase expression
EDAT- 2020/08/23 06:00
MHDA- 2021/05/29 06:00
CRDT- 2020/08/23 06:00
PHST- 2020/08/23 06:00 [entrez]
PHST- 2020/08/23 06:00 [pubmed]
PHST- 2021/05/29 06:00 [medline]
AID - 50/4/439 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2020 Jul;50(4):439-446.