PMID- 32827326
OWN - NLM
STAT- MEDLINE
DCOM- 20210709
LR  - 20210709
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 44
IP  - 12
DP  - 2020 Dec
TI  - Co-cultured bone marrow mesenchymal stem cells repair thioacetamide-induced 
      hepatocyte damage.
PG  - 2459-2472
LID - 10.1002/cbin.11453 [doi]
AB  - Adult stem cells, such as bone marrow mesenchymal stem cells (BMSCs), are 
      postdevelopmental cells found in many bone tissues. They are capable of 
      multipotent differentiation and have low immune-rejection characteristics. 
      Hepatocytes may become inflamed and produce a large number of free radicals when 
      affected by drugs, poisoning, or a viral infection. The excessive accumulation of 
      free radicals in the extracellular matrix (ECM) eventually leads to liver 
      fibrosis. This study aims to investigate the restorative effects of mouse bone 
      marrow mesenchymal stem cells (mBMSCs) on thioacetamide (TAA)-induced damage in 
      hepatocytes. An in vitro transwell co-culture system of HepG2 cells were 
      co-cultured with mBMSCs. The effects of damage done to TAA-treated HepG2 cells 
      were reflected in the overall cell survival, the expression of antioxidants 
      (SOD1, GPX1, and CAT), the ECM (COL1A1 and MMP9), antiapoptosis characteristics 
      (BCL2), and inflammation (TNF) genes. The majority of the damage done to HepG2 by 
      TAA was significantly reduced when cells were co-cultured with mBMSCs. The signal 
      transducer and activator of transcription 3 (STAT3) and its phosphorylated STAT3 
      (p-STAT3), as related to cell growth and survival, were detected in this study. 
      The results show that STAT3 was significantly decreased in the TAA-treated HepG2 
      cells, but the STAT3 and p-STAT3 of HepG2 cells were significantly activated when 
      the TAA-treated HepG2 co-cultured with mBMSCs. Strong expression of interleukin 
      (Il6) messenger RNA in co-cultured mBMSCs/HepG2 indicated mBMSCs secret the 
      cytokines IL-6, which promotes cell survival through downstream STAT3 activation 
      and aid in the recovery of HepG2 cells damaged by TAA.
CI  - (c) 2020 International Federation for Cell Biology.
FAU - Chen, Hung-Chiuan
AU  - Chen HC
AD  - Department of BioAgricultural Sciences, National Chiayi University, Chiayi City, 
      Taiwan.
FAU - Awale, Suresh
AU  - Awale S
AUID- ORCID: 0000-0002-5299-193X
AD  - Department of Translational Research, Institute of Natural Medicine, University 
      of Toyama, Toyama, Japan.
FAU - Wu, Chean-Ping
AU  - Wu CP
AD  - Department of Animal Science, National Chiayi University, Chiayi City, Taiwan.
FAU - Lee, Hu-Hui
AU  - Lee HH
AD  - Department of BioAgricultural Sciences, National Chiayi University, Chiayi City, 
      Taiwan.
FAU - Wu, Hsi-Tien
AU  - Wu HT
AUID- ORCID: 0000-0001-8527-9597
AD  - Department of BioAgricultural Sciences, National Chiayi University, Chiayi City, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20200831
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 075T165X8M (Thioacetamide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation
MH  - China
MH  - Coculture Techniques
MH  - Hep G2 Cells
MH  - Hepatocytes/drug effects/*metabolism
MH  - Humans
MH  - Liver Cirrhosis/pathology
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Phosphorylation
MH  - STAT3 Transcription Factor/metabolism
MH  - Signal Transduction/drug effects
MH  - Thioacetamide/adverse effects/pharmacology
OTO - NOTNLM
OT  - STAT3
OT  - bone marrow mesenchymal stem cells
OT  - hepatocyte HepG2
OT  - thioacetamide
OT  - transwell co-culture system
EDAT- 2020/08/23 06:00
MHDA- 2021/07/10 06:00
CRDT- 2020/08/23 06:00
PHST- 2020/03/28 00:00 [received]
PHST- 2020/07/18 00:00 [revised]
PHST- 2020/08/16 00:00 [accepted]
PHST- 2020/08/23 06:00 [pubmed]
PHST- 2021/07/10 06:00 [medline]
PHST- 2020/08/23 06:00 [entrez]
AID - 10.1002/cbin.11453 [doi]
PST - ppublish
SO  - Cell Biol Int. 2020 Dec;44(12):2459-2472. doi: 10.1002/cbin.11453. Epub 2020 Aug 
      31.